LOCUS       sequence-175412-       12254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gateway-based Bimolecular Fluorescence Complementation (BiFC) binary
            vector containing N terminal fragment of YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12254)
  AUTHORS   Belda-Palazon B, Ruiz L, Marti E, Tarraga S, Tiburcio AF, Culianez 
            F, Farras R, Carrasco P, Ferrando A
  TITLE     Aminopropyltransferases involved in polyamine biosynthesis localize 
            preferentially in the nucleus of plant cells.
  JOURNAL   PLoS One. 2012;7(10):e46907. doi: 10.1371/journal.pone.0046907. Epub
            2012 Oct 8.
  PUBMED    23056524
REFERENCE   2  (bases 1 to 12254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(10):e46907. doi"; pages: " 
            10.1371/journal.pone.0046907. Epub 2012 Oct 8"
COMMENT     Sequence Label: YFN43
FEATURES             Location/Qualifiers
     source          1..12254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             join(373..375,376..378,379..837)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(418..439)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(679..698)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    904..1028
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1053..1058,1059..1076,1077..1083)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1137..1817
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(1203..1222)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2137..2442
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2321..2340
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2483..2607)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2671..2923
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2940..2956)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2940..2956)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2953..2975)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2964..2980
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2988..2994,2995..3012,3013..3018))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3033..3054
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3245..3921
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             3988..5013
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    5054..5228
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5306..5330
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5755..6549
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6636..7224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7125..7144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7378..7395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7410..7550
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7476..7495)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7636..7658
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7894..8088
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8154..9227)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9656..10285)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11586..11610
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     11798..11820
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11812..11829
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11813..11829
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacctcga ctctagagga
      361 tccccgggta ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      421 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      481 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      541 ccctggccca ccctcgtgac caccttcggc tacggcctgc agtgcttcgc ccgctacccc
      601 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      661 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      721 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      781 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccggt
      841 ggaggtggat ctggtggagg tggatctggt ggaggtggat ctcttggcgc gccaagctat
      901 caaacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata
      961 ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag
     1021 tcactatggc ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata
     1081 atgtgtggat tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg
     1141 agaaaaaaat cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt
     1201 ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta
     1261 cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca
     1321 ttcttgcccg cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc
     1381 tggtgatatg ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt
     1441 tttcatcgct ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc
     1501 aagatgtggc gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata
     1561 tgtttttcgt ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca
     1621 atatggacaa cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca
     1681 aggtgctgat gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg
     1741 gcagaatgct taatgaatta caacagtact gcgatgagtg gcaggcgggg cgtaatctag
     1801 aggatccggc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
     1861 tataagaata tatactgata tgtatacccg aagtatgtca aaaagaggta tgctatgaag
     1921 cagcgtatta cagtgacagt tgacagcgac agctatcagt tgctcaaggc atatatgatg
     1981 tcaatatctc cggtctggta agcacaacca tgcagaatga agcccgtcgt ctgcgtgccg
     2041 aacgctggaa agcggaaaat caggaaggga tggctgaggt cgcccggttt attgaaatga
     2101 acggctcttt tgctgacgag aacaggggct ggtgaaatgc agtttaaggt ttacacctat
     2161 aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat tgacacgccc
     2221 gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa agtcccccgt
     2281 gaactttacc cggtggtgca tatcggggat gaaagctggc gcatgatgac caccgatatg
     2341 gccagtgtgc cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat
     2401 gacatcaaaa acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata
     2461 cacagccagt ctgcaggtcg accatagtga ctggatatgt tgtgttttac agtattatgt
     2521 agtctgtttt ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc
     2581 tcgttcagct ttcttgtaca aagtggttcg ataattctta attaactagt tctagagcgg
     2641 ccgccaccgc ggtggagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
     2701 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
     2761 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
     2821 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
     2881 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaatt cgtaatcatg
     2941 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     3001 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     3061 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     3121 ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc aacatggtgg
     3181 agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa gaccaaaggg
     3241 ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc cattgcccag
     3301 ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac aaatgccatc
     3361 attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt cccaaagatg
     3421 gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc
     3481 aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa gaatatcaaa
     3541 gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt aatatcggga
     3601 aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac agtagaaaag
     3661 gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt tcaagatgcc
     3721 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
     3781 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac tgacgtaagg
     3841 gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg aagttcattt
     3901 catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc tctctcgagc
     3961 tttcgcagat ccggggggca atgagatatg aaaaagcctg aactcaccgc gacgtctgtc
     4021 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     4081 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     4141 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     4201 ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac ctattgcatc
     4261 tcccgccgtt cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     4321 ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag ccagacgagc
     4381 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     4441 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     4501 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     4561 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     4621 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     4681 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     4741 aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg cattggtctt
     4801 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     4861 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     4921 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     4981 cgccccagca ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg atctgtcgat
     5041 cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag ggaattaggg
     5101 ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     5161 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa
     5221 tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt
     5281 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc
     5341 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag
     5401 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
     5461 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
     5521 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc gcggtttcaa
     5581 aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt
     5641 tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata
     5701 attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct
     5761 aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat
     5821 acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat
     5881 ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac
     5941 atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat
     6001 gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat
     6061 gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt
     6121 cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa
     6181 ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac
     6241 actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag
     6301 gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa
     6361 gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc
     6421 ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt
     6481 gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa
     6541 ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc gttccactga
     6601 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6661 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6721 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6781 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6841 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6901 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6961 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7021 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7081 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7141 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7201 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7261 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7321 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     7381 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
     7441 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag
     7501 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc
     7561 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     7621 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     7681 gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt
     7741 ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg
     7801 ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt
     7861 tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga
     7921 gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca
     7981 gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc
     8041 cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct
     8101 tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa
     8161 ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc
     8221 tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc
     8281 acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg
     8341 aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg
     8401 ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg
     8461 ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc
     8521 ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc
     8581 accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac
     8641 cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac
     8701 ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc
     8761 cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc
     8821 cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg
     8881 tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc
     8941 atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt
     9001 tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg
     9061 gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc
     9121 tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc
     9181 agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc gattcctcgg
     9241 gcttgggggt tccagtgcca ttgcagggcc ggcaggcaac ccagccgctt acgcctggcc
     9301 aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat
     9361 tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt
     9421 tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt
     9481 accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc
     9541 atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga
     9601 cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa
     9661 tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg
     9721 gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg
     9781 tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc
     9841 cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg
     9901 tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag
     9961 ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt
    10021 ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt
    10081 cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg
    10141 ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg
    10201 cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct
    10261 ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg
    10321 catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt
    10381 tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc
    10441 atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc
    10501 gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc
    10561 gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc
    10621 gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg
    10681 gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca
    10741 agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc
    10801 gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg
    10861 gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg
    10921 gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc
    10981 aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg
    11041 gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg
    11101 tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac
    11161 agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg
    11221 gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt
    11281 ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac aagaaaacgc
    11341 caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga catgtcgttt
    11401 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg
    11461 atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga
    11521 acggataaac cttttcacgc ccttttaaat atccgattat tctaataaac gctcttttct
    11581 cttaggttta cccgccaata tatcctgtca aacactgata gtttaaactg aaggcgggaa
    11641 acgacaatct gatccaagct caagctgctc tagcattcgc cattcaggct gcgcaactgt
    11701 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
    11761 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
    11821 acggccagtg ccaagcttgc atgcctgcag gtcaacatgg tggagcacga cacacttgtc
    11881 tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga gacttttcaa
    11941 caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg tcactttatt
    12001 gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag
    12061 gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg
    12121 agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat
    12181 aacatggtgg agcacgacac acttgtctac tccaaaaata tcaaagatac agtctcagaa
    12241 gaccaaaggg caat
//
