LOCUS       sequence-175413-       12050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  gateway-based Bimolecular Fluorescence Complementation (BiFC) binary
            vector containing C terminal fragment of YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12050)
  AUTHORS   Belda-Palazon B, Ruiz L, Marti E, Tarraga S, Tiburcio AF, Culianez 
            F, Farras R, Carrasco P, Ferrando A
  TITLE     Aminopropyltransferases involved in polyamine biosynthesis localize 
            preferentially in the nucleus of plant cells.
  JOURNAL   PLoS One. 2012;7(10):e46907. doi: 10.1371/journal.pone.0046907. Epub
            2012 Oct 8.
  PUBMED    23056524
REFERENCE   2  (bases 1 to 12050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(10):e46907. doi"; pages: " 
            10.1371/journal.pone.0046907. Epub 2012 Oct 8"
COMMENT     Sequence Label: YFC43
FEATURES             Location/Qualifiers
     source          1..12050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..345
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             379..630
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     567..588
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    700..824
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(849..854,855..872,873..879)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             933..1613
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     primer_bind     complement(999..1018)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1933..2238
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2117..2136
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2279..2403)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      2467..2719
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2736..2752)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2736..2752)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2749..2771)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2760..2776
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2784..2790,2791..2808,2809..2814))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2829..2850
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3041..3717
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             3784..4809
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    4850..5024
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5102..5126
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5551..6345
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      6432..7020
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6921..6940
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7174..7191
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7206..7346
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7272..7291)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7432..7454
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7690..7884
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7950..9023)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9452..10081)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    11382..11406
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     11594..11616
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11608..11625
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11609..11625
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag aggacctcga ctctagagga
      361 tccccgggta ccatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
      421 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      481 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc cgccctgagc
      541 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      601 atcactctcg gcatggacga gctgtacaag gccggtggag gtggatctgg tggaggtgga
      661 tctggtggag gtggatctct tggcgcgcca agctatcaaa caagtttgta caaaaaagct
      721 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
      781 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca
      841 ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc
      901 cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca
      961 ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc
     1021 aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga
     1081 aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc
     1141 atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc
     1201 cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc
     1261 acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa
     1321 acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct
     1381 gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg
     1441 ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc
     1501 aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac
     1561 agtactgcga tgagtggcag gcggggcgta atctagagga tccggcttac taaaagccag
     1621 ataacagtat gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta
     1681 tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac
     1741 agcgacagct atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca
     1801 caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg
     1861 aagggatggc tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca
     1921 ggggctggtg aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg
     1981 tttgtggatg tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg
     2041 gccagtgcac gtctgctgtc agataaagtc ccccgtgaac tttacccggt ggtgcatatc
     2101 ggggatgaaa gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc
     2161 ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg
     2221 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca
     2281 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
     2341 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt
     2401 ggttcgataa ttcttaatta actagttcta gagcggccgc caccgcggtg gagctcgaat
     2461 ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc tgttgccggt
     2521 cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat aattaacatg
     2581 taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca attatacatt
     2641 taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc gcgcgcggtg
     2701 tcatctatgt tactagatcg ggaattcgta atcatgtcat agctgtttcc tgtgtgaaat
     2761 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     2821 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     2881 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     2941 ttgcgtattg gctagagcag cttgccaaca tggtggagca cgacactctc gtctactcca
     3001 agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg
     3061 taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga
     3121 cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg
     3181 ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
     3241 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gaacatggtg
     3301 gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg
     3361 gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca
     3421 gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat
     3481 cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat
     3541 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
     3601 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct
     3661 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaaat
     3721 caccagtctc tctctacaaa tctatctctc tcgagctttc gcagatccgg ggggcaatga
     3781 gatatgaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
     3841 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
     3901 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
     3961 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
     4021 attggggagt ttagcgagag cctgacctat tgcatctccc gccgttcaca gggtgtcacg
     4081 ttgcaagacc tgcctgaaac cgaactgccc gctgttctac aaccggtcgc ggaggctatg
     4141 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
     4201 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
     4261 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
     4321 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
     4381 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
     4441 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
     4501 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atccggagct tgcaggatcg
     4561 ccacgactcc gggcgtatat gctccgcatt ggtcttgacc aactctatca gagcttggtt
     4621 gacggcaatt tcgatgatgc agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc
     4681 ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat
     4741 ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc ccagcactcg tccgagggca
     4801 aagaaataga gtagatgccg accgggatct gtcgatcgac aagctcgagt ttctccataa
     4861 taatgtgtga gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg
     4921 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
     4981 atttctaatt cctaaaacca aaatccagta ctaaaatcca gatcccccga attaattcgg
     5041 cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt
     5101 tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg
     5161 gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag
     5221 ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta
     5281 agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg
     5341 acagagcgtt gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt
     5401 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
     5461 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
     5521 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
     5581 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
     5641 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
     5701 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
     5761 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
     5821 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
     5881 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
     5941 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
     6001 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
     6061 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
     6121 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     6181 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     6241 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     6301 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agaatgcatg
     6361 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6421 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6481 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6541 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     6601 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6661 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     6721 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     6781 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     6841 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     6901 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     6961 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7021 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7081 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7141 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7201 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     7261 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     7321 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     7381 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     7441 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt
     7501 gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc
     7561 cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg
     7621 cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg
     7681 ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt
     7741 taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc
     7801 ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg
     7861 ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg
     7921 gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc
     7981 aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg
     8041 tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac
     8101 tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg
     8161 cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag
     8221 taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc
     8281 caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg
     8341 tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc
     8401 ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg
     8461 tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga
     8521 cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt
     8581 agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc
     8641 cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt
     8701 cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg
     8761 cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc
     8821 ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg
     8881 gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc
     8941 tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg
     9001 ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc
     9061 agggccggca ggcaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg
     9121 ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg
     9181 ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat
     9241 tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg
     9301 tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg
     9361 gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg
     9421 cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg
     9481 ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc
     9541 cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca
     9601 gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc
     9661 gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca
     9721 gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca
     9781 cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga
     9841 tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga
     9901 tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct
     9961 ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat
    10021 gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca
    10081 tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg
    10141 acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc
    10201 agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc
    10261 atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat
    10321 ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt
    10381 catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat
    10441 gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc
    10501 tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg
    10561 gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta
    10621 gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg
    10681 tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg
    10741 ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc
    10801 tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc
    10861 ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg
    10921 cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc
    10981 agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc
    11041 aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc
    11101 tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc
    11161 tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt
    11221 cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg
    11281 gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt
    11341 ttaaatatcc gattattcta ataaacgctc ttttctctta ggtttacccg ccaatatatc
    11401 ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga caatctgatc caagctcaag
    11461 ctgctctagc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
    11521 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
    11581 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc
    11641 ctgcaggtca acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca
    11701 gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc
    11761 ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt
    11821 ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc
    11881 gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt
    11941 ccaaccacgt cttcaaagca agtggattga tgtgataaca tggtggagca cgacacactt
    12001 gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
//
