LOCUS       sequence-175414-       10841 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for reporter assay of one zebrafish super-enhancer region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10841)
  AUTHORS   Perez-Rico YA, Boeva V, Mallory AC, Bitetti A, Majello S, Barillot 
            E, Shkumatava A
  TITLE     Comparative analyses of super-enhancers reveal conserved elements in
            vertebrate genomes.
  JOURNAL   Genome Res. 2017 Feb;27(2):259-268. doi: 10.1101/gr.203679.115. Epub
            2016 Dec 13.
  PUBMED    27965291
REFERENCE   2  (bases 1 to 10841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.203679.115"; journalName: "Genome Res"; date: "2017-02";
            volume: "27"; issue: "2"; pages: "259-268"
COMMENT     Sequence Label: E1b-GFP-Tol2-Gateway dre SE-irf2bpl B
FEATURES             Location/Qualifiers
     source          1..10841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..125
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(150..155,156..173,174..180)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             234..893
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(300..319)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1235..1540
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1419..1438
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1581..1705)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          3333..3905
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      3979..3988
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3985..3987,3988..3990,3991..4704)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3985..3987,3988..3990,3991..4701)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4030..4051)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4291..4310)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4638..4659
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4749..4883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4773..4792)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4827..4846
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(5320..5507,5508..5519)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5553..5569)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5553..5569)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5606..5626)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5606..5624)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5645..5661)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5645..5661)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5658..5680)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5669..5685
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5693..5699,5700..5717,5718..5723))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5738..5759
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5876..5893)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6047..6635)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6127..6146)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6806..7597,7598..7666))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7429..7448
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7667..7771)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7797..8252)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7934..7955)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8146..8165
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8379..8401
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8393..8410
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8394..8410
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8420..8439
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8420..8438
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(10289..10300,10301..10463)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
       61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      121 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      181 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
      241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
      781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
      901 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      961 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1021 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1081 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1141 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1201 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     1261 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1321 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1381 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1441 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1501 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1561 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1681 gttcagcttt cttgtacaaa gtggtgatat caagatctcc gaaccgaaca tgaagtttgg
     1741 cataaacaaa acatcattat attacacccg cagtatttta ggctgttacc aaaaacacac
     1801 ttgaacacac ctgagtaatt tctaagaaag atcatacata acaaatgcag gaaaaaaaca
     1861 tagtctaatt gattagcata acaagctaat aaaaattatg tttgatattg taattattta
     1921 gaaaaatgca actatttatt gaacgttgct gctaaaatat ggttgaacac actttaggca
     1981 agcgatgtac tcttaacagc aggggaaaaa cactagaaaa tgtacagcct cacatggcgt
     2041 gctgctttaa taaacaacag agcactcaaa ggtgaataaa atacagctct gagtacagtg
     2101 gatggtcacc agattgaaga atgagagact gtttttgtgt gtgtgtgtgt gagagagaga
     2161 gagggggaag ggccccgatg gaagatggat gacagaacaa gccgctgact gaactcgctg
     2221 cactgtgaaa gcctaagaaa cccgagaccg ctctctgtta gcagagctcc agtctaaaat
     2281 ggctgccagt cgtgtaaaca acccagagaa tgaaaggggg ctacatttct ccagtgagct
     2341 ggccaagagc cgcacagctc gtgggttaca aaattaaccc tttccttcca accaggcagc
     2401 acgtcggata aaaacaaagg aaggaatgta gcactggtgg tttgtagggc tgcatgatat
     2461 caggggggag gaaatatgac gttgcgatat tttgcttgtc tgcgatatac agctattgag
     2521 attttaatat aatttcacag gatgacttta ttggaaagta ttcattttag tttgactggg
     2581 gttattttgc aggtatgtgc aaagattttg aatataattt cacaagacaa ctttattgat
     2641 taataaatca acatttttaa ttgattgggg gttattttgt aggggagttt ctgcgtaaaa
     2701 tatgataaac aacaaggaca gatgaatata caaagatata aatgaaataa acagtgaatt
     2761 acgactttga atataatttc aaaagattac tttgagaaaa aaatcttcat tttagattga
     2821 ttgtggttat tttgtagagg aatatctgca taaaatataa taaactacaa ggaaagatga
     2881 atgtataaac gcataaatga aataaacagt gttttatgat tcattaattc attttctttt
     2941 tggcttagtc cctttattaa tctggggacg ccacagcaga atgaaccgtc aacttatcca
     3001 gcatatgttt tacgcagtgg atgcccttcc agccgcaacc ctccactggg aaactcattc
     3061 acacacacac acacacacac acacactcac tcatacacta cggtcaattt tagcttacac
     3121 aattgacctg taccacatgt ctttggactt gtgggagaaa ccggagcacc cagaggaaac
     3181 cctcgcgaac acagggagaa catgcaaact ccaactgaac gccaactgac ccagatctct
     3241 cgactctaga gggtatataa tggatcccat cgcgtctcag cctcactttg agctcctcca
     3301 cacgaatttc gaccgatcct gagaacttca gggtgagttt ggggaccctt gattgttctt
     3361 tctttttcgc tattgtaaaa ttcatgttat atggaggggg caaagttttc agggtgttgt
     3421 ttagaatggg aagatgtccc ttgtatcacc atggaccctc atgataattt tgtttctttc
     3481 actttctact ctgttgacaa ccattgtctc ctcttatttt cttttcattt tctgtaactt
     3541 tttcgttaaa ctttagcttg catttgtaac gaatttttaa attcactttt gtttatttgt
     3601 cagattgtaa gtactttctc taatcacttt tttttcaagg caatcagggt atattatatt
     3661 gtacttcagc acagttttag agaacaattg ttataattaa atgataaggt agaatatttc
     3721 tgcatataaa ttctggctgg cgtggaaata ttcttattgg tagaaacaac tacaccctgg
     3781 tcatcatcct gcctttctct ttatggttac aatgatatac actgtttgag atgaggataa
     3841 aatactctga gtccaaaccg ggcccctctg ctaaccatgt tcatgccttc ttctctttcc
     3901 tacagctcct gggcaacgtg ctggttgttg tgctgtctca tcattttggc aaagaattcc
     3961 tcgacggatc caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
     4021 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     4081 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     4141 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     4201 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     4261 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     4321 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     4381 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     4441 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     4501 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     4561 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     4621 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     4681 ggcatggacg agctgtacaa gtaaagcggc cgccaccgcg gtggagctcg aattaattca
     4741 tcgatgatga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     4801 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     4861 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     4921 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
     4981 attatgatcc tctagatcag atccgaagat acggccacgg gtgctcttga tcctgtggct
     5041 gattttggac tgtgctgctc gcagctgctg atgaatcaca tacttcctcc attttcttcc
     5101 actgattgac tgttataatt tccctaattt ccaggtcaag gtgctgtgca ttgtggtaat
     5161 agatgtgaca tgacgtcact tccaaaggac caatgaacat gtctgaccaa tttcatataa
     5221 tgtgaaaacg attttcatag gcagaataaa taacatttaa attaaactgg gcatcagcgc
     5281 aattcaattg gtttggtaat agcaagggaa aatagaatga agtgatctcc aaaaaataag
     5341 tactttttga ctgtaaataa aattgtaagg agtaaaaagt actttttttt ctaaaaaaat
     5401 gtaattaagt aaaagtaaaa gtattgattt ttaattgtac tcaagtaaag taaaaatccc
     5461 caaaaataat acttaagtac agtaatcaag taaaattact caagtacttt acacctctgg
     5521 ttcttgaccc cctaccttca gcaagcccag cagatccact agttctagag cggccgccac
     5581 cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat
     5641 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     5701 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     5761 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     5821 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     5881 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     5941 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     6001 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     6061 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     6121 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     6181 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     6241 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     6301 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     6361 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     6421 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     6481 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     6541 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     6601 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     6661 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     6721 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     6781 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     6841 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     6901 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     6961 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     7021 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     7081 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     7141 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     7201 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     7261 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     7321 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     7381 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     7441 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     7501 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     7561 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     7621 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     7681 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     7741 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctaaat
     7801 tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt
     7861 taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg
     7921 gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt
     7981 caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc
     8041 aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg
     8101 atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa
     8161 aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc
     8221 cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc tgcgcaactg
     8281 ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg
     8341 tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac
     8401 gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta ccaaatagta
     8461 ggaattaccc acctgtacaa gtgctgaaaa cttggatgaa taagcccgtt tgccattttc
     8521 tactgctatt ttaaatcttt tctgtatttg tctgcatttg tctttaccct tgaataaatg
     8581 ttttagttgt tttttttcaa ttatgactgt gtttaacaga cattattaca ataatatgta
     8641 atagtagtac acactattat tatgtaatag tacttgttga ctgtatttga gaactggacc
     8701 gagtgagtgt tacgtcaccc attcaaaatg acttacttct ggctccaaca aaatgaagtt
     8761 aattcagttg ccatttttca ctgtatggac atcgccgtgt tggagctaga cgttgccaat
     8821 aagcaagaaa gagccgagat gcgtcgagtc tgagtcaccg ttcctatggc aacccctcta
     8881 accaatcaga agtaagcttg ttggaagtcc acagcctacc acttgaaagc gggctgcaca
     8941 aaatctgtca aacgttttga acgttggatg tgagagcaca tacttttatt aaggcatctg
     9001 gttggtcagt ttataatatc aacaacttgg gctacagaaa agaaaagtta ttacagaaat
     9061 tatgattaac aagtacatgt taaataaaga ttttaatatg aatgccacca ctggagcatt
     9121 catgccattt ggagcttctt cctgtttgga tcactagaag gaggaggtca ctcattacag
     9181 ttctcatata cagtcgttgg ttggttggtt ggttggtaga ttgattgatt gattgattga
     9241 ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt gattggtagt
     9301 caaaataaga aataatttcc acagattcat tacagaaatg attaaatgca tacataaaaa
     9361 actggggggg ggggggatac aacaacacac ttaagtcaca tttgcctacg taaagaaaag
     9421 taaagaaaat caatagctat attttacatc tccttttttt gctgtctttt aattagcctt
     9481 gttttgctgt tatcttgatt gaaactgtaa cttcttcacc tgcttctttt ctttgtaggt
     9541 tttgccagcc cagaaacagg catggctgtg caaggccaga gcaccatgca caatgcgctg
     9601 catgtcttca tgaacggctc aatgtcctca gtccagggct cagccaacga ccccatcttc
     9661 ctccttcacc atgctttcat tgacaggtaa caaacacgtc atgacattag actgcacagt
     9721 ttttgacaaa gttcatacaa tctgttgttt atagctgcta caattagtga agtttgtgaa
     9781 tgtacttgga tgagcagcga aagatcaatt gagatcaatt gttagagttt ggttgccctg
     9841 cagagcaaag aacaaaaaat aatctggtgg ctttactgcg tgaggttatt attggtggaa
     9901 tagaaacaca aaacataatt gcatttattt gtttaatttt ttatcttatc ttaactttca
     9961 tcttgcatat ttgtttctta catcatttct agcatctttg agcgctggct aagaactcat
    10021 cagcctcccc ggtccatcta cccacgtacc aatgcaccaa ttggccacaa tgacggctac
    10081 tacatggtgc cattccttcc tctttatagg aatggagact acctcctgtc caacaatgct
    10141 cttggatacg agtacgccta cctgttggac ccaggtcatt gcacaacacc agaaatgccc
    10201 tctgatctgc aaaagacgtg aatatctgtt cagacaccca tatccactct gttccacaca
    10261 ggtcagaggt ttgtccagga gttcttgaca gaggtgtaaa aagtactcaa aaattttact
    10321 caagtgaaag tacaagtact tagggaaaat tttactcaat taaaagtaaa agtatctggc
    10381 tagaatctta cttgagtaaa agtaaaaaag tactccatta aaattgtact tgagtattaa
    10441 ggaagtaaaa gtaaaagcaa gaaagaaaac tagagattct tgtttaagct tttaatctca
    10501 aaaaacatta aatgaaatgc atacaaggtt ttatcctgct ttagaactgt ttgtatttaa
    10561 ttatcaaact ataagacaga caatctaatg ccagtacacg ctactcaaag ttgtaaaacc
    10621 tcagatttaa cttcagtaga agctgattct caaaattgtt agtgtcaagc ctagctcttt
    10681 tggggctgaa aagcaatcct gcagtgctga aaagcctctc acaggcagcc gatgcgggaa
    10741 gaggtgtatt agtcttgata gagaggctgc aaatagcagg aaacgtgagc agagactccc
    10801 tggtgtctga aacacaggcc agatgggccc tcgaggatat c
//
