LOCUS       sequence-175415-       11069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for reporter assay of one zebrafish super-enhancer region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11069)
  AUTHORS   Perez-Rico YA, Boeva V, Mallory AC, Bitetti A, Majello S, Barillot 
            E, Shkumatava A
  TITLE     Comparative analyses of super-enhancers reveal conserved elements in
            vertebrate genomes.
  JOURNAL   Genome Res. 2017 Feb;27(2):259-268. doi: 10.1101/gr.203679.115. Epub
            2016 Dec 13.
  PUBMED    27965291
REFERENCE   2  (bases 1 to 11069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.203679.115"; journalName: "Genome Res"; date: "2017-02";
            volume: "27"; issue: "2"; pages: "259-268"
COMMENT     Sequence Label: E1b-GFP-Tol2-Gateway dre SE-zic2a R
FEATURES             Location/Qualifiers
     source          1..11069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..125
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(150..155,156..173,174..180)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             234..893
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(300..319)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1235..1540
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1419..1438
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1581..1705)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          3561..4133
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      4207..4216
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4213..4215,4216..4218,4219..4932)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4213..4215,4216..4218,4219..4929)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4258..4279)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4519..4538)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4866..4887
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4977..5111
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5001..5020)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5055..5074
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(5548..5735,5736..5747)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5781..5797)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5781..5797)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5834..5854)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5834..5852)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5873..5889)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5873..5889)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5886..5908)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5897..5913
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5921..5927,5928..5945,5946..5951))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5966..5987
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6104..6121)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6275..6863)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6355..6374)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7034..7825,7826..7894))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7657..7676
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7895..7999)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8025..8480)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8162..8183)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8374..8393
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8607..8629
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8621..8638
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8622..8638
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8648..8667
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8648..8666
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(10517..10528,10529..10691)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
       61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      121 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      181 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
      241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
      781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
      901 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      961 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1021 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1081 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1141 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1201 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     1261 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1321 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1381 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1441 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1501 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1561 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1681 gttcagcttt cttgtacaaa gtggtgatat caagatctga tttccaaagt atcaagtgac
     1741 acaatttgct ataatagcaa taaattacat tttaaaaatt atttaaaatt ataataatct
     1801 tttgttatta ttattgaaat attactattc attatataca gttcaagtca gaattattag
     1861 ccccccctgt atatttttcc cccaattttt gtttaatgga ataaagattt ttttcaacac
     1921 atttctaaac ataataattt taataactca tttctaatag ctaatttatt taagacatta
     1981 gtatttagct taaagggcaa tttaatggca taactatagg ttaattcagt taattaggca
     2041 agtttgggta tttaggcaaa tctttgtata atgatatgtt ctgttgataa tcaaaaacaa
     2101 tattgcttga gggggctaat aacatagacc ttaaaatgct ttctaaaaaa aactgctttt
     2161 attctagccg aaataaaaca aataagactt tctccagaag aaaaaatatt attggaatac
     2221 tgtcaaaaat tccttgctct attaatcata gtttgggaaa tattcgaaaa agaaaagaag
     2281 aaaatactta aactgtatat aacatttttt ataaaaacaa aaatgtatac aatttgtaat
     2341 agtttttata ccaatgatag cccatttaaa aaaaaaataa aagtccatct aaatctataa
     2401 aacagataat atgtaaagtt ttagcattaa aaatcaagtg taaaaaaaac tgtaaaattg
     2461 tccatgaatc ataagtgctt gctattcagt tttgatcaaa tacatggagc taaggtatgc
     2521 atgttatcaa accagttgcc cttgacgttg cccagttcac tccaaagagg tacactccac
     2581 acaataattt aacccttcaa atattaaatc acacattgat ttatgttgaa taagggtgca
     2641 tgttaataca ttgaccgatg aacacattgt atggatctcc tgatgaatcc tcctttctcc
     2701 ttctcccccg tccattgatt gtccacgcca caagccccaa gaggaagctt atttggggcc
     2761 tgtctgcagc gtgagggtgt gtaagggagc acagggtggt cctgtgggcc agagagggca
     2821 gctaactgga gtaaacagga ctcagcaggc ttagtgtttg tgtttactct gctggagagg
     2881 aggccattag agcaaacagc cagcgtttct tcacatgttg ctgaaaatca ccccaacctt
     2941 cattcactcc tgtttggcac tatactaaca cccagccagt gtgcccagat ggccccgctt
     3001 tccagatctc tcactgtttc tgccctctct tcccctcctt ccctgtgccg tggcgtcatt
     3061 aaaagtgtct gtgagcggga ggtgtcaaaa aaagggtgcc gaaagagaga gagcagaaaa
     3121 caatttagtg gcatttaaag ataaagtcct ccccctattt acatggccta tctaatccaa
     3181 tctaggctgt gtctgcattt ttaatttgca cattcaagcg agcttcagag aggaacctag
     3241 gcttaattag tttagttatc tcccctcatg aatggataaa gttcccacac tgctggtgtt
     3301 tcagatgaag actaattgga ttctgaggcc aaaaacaaaa ggagaaataa aagaaacaag
     3361 ggttagataa cagagggaag attgaattga taaagcgcaa ggagagtgaa ctgtagtcac
     3421 agctaaggag ggagaaaagt ccagattcct gtttgaggtc agatctctcg actctagagg
     3481 gtatataatg gatcccatcg cgtctcagcc tcactttgag ctcctccaca cgaatttcga
     3541 ccgatcctga gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta
     3601 ttgtaaaatt catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa
     3661 gatgtccctt gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct
     3721 gttgacaacc attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact
     3781 ttagcttgca tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt
     3841 actttctcta atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac
     3901 agttttagag aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt
     3961 ctggctggcg tggaaatatt cttattggta gaaacaacta caccctggtc atcatcctgc
     4021 ctttctcttt atggttacaa tgatatacac tgtttgagat gaggataaaa tactctgagt
     4081 ccaaaccggg cccctctgct aaccatgttc atgccttctt ctctttccta cagctcctgg
     4141 gcaacgtgct ggttgttgtg ctgtctcatc attttggcaa agaattcctc gacggatcca
     4201 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     4261 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     4321 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     4381 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     4441 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     4501 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     4561 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     4621 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     4681 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     4741 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4801 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     4861 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4921 ctgtacaagt aaagcggccg ccaccgcggt ggagctcgaa ttaattcatc gatgatgatc
     4981 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa
     5041 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca
     5101 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt
     5161 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc
     5221 tagatcagat ccgaagatac ggccacgggt gctcttgatc ctgtggctga ttttggactg
     5281 tgctgctcgc agctgctgat gaatcacata cttcctccat tttcttccac tgattgactg
     5341 ttataatttc cctaatttcc aggtcaaggt gctgtgcatt gtggtaatag atgtgacatg
     5401 acgtcacttc caaaggacca atgaacatgt ctgaccaatt tcatataatg tgaaaacgat
     5461 tttcataggc agaataaata acatttaaat taaactgggc atcagcgcaa ttcaattggt
     5521 ttggtaatag caagggaaaa tagaatgaag tgatctccaa aaaataagta ctttttgact
     5581 gtaaataaaa ttgtaaggag taaaaagtac ttttttttct aaaaaaatgt aattaagtaa
     5641 aagtaaaagt attgattttt aattgtactc aagtaaagta aaaatcccca aaaataatac
     5701 ttaagtacag taatcaagta aaattactca agtactttac acctctggtt cttgaccccc
     5761 taccttcagc aagcccagca gatccactag ttctagagcg gccgccaccg cggtggagct
     5821 ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc
     5881 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     5941 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6001 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6061 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6121 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6181 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6241 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6301 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6361 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6421 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6481 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6541 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6601 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6661 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6721 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6781 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6841 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6901 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     6961 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7021 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7081 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7141 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7201 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7261 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7321 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7381 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7441 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7501 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7561 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7621 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7681 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7741 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7801 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7861 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     7921 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     7981 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa
     8041 tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc
     8101 cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt
     8161 tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa
     8221 aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg
     8281 gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg
     8341 acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc
     8401 tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa
     8461 tgcgccgcta cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg
     8521 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
     8581 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga
     8641 gcgcgcgtaa tacgactcac tatagggcga attgggtacc aaatagtagg aattacccac
     8701 ctgtacaagt gctgaaaact tggatgaata agcccgtttg ccattttcta ctgctatttt
     8761 aaatcttttc tgtatttgtc tgcatttgtc tttacccttg aataaatgtt ttagttgttt
     8821 tttttcaatt atgactgtgt ttaacagaca ttattacaat aatatgtaat agtagtacac
     8881 actattatta tgtaatagta cttgttgact gtatttgaga actggaccga gtgagtgtta
     8941 cgtcacccat tcaaaatgac ttacttctgg ctccaacaaa atgaagttaa ttcagttgcc
     9001 atttttcact gtatggacat cgccgtgttg gagctagacg ttgccaataa gcaagaaaga
     9061 gccgagatgc gtcgagtctg agtcaccgtt cctatggcaa cccctctaac caatcagaag
     9121 taagcttgtt ggaagtccac agcctaccac ttgaaagcgg gctgcacaaa atctgtcaaa
     9181 cgttttgaac gttggatgtg agagcacata cttttattaa ggcatctggt tggtcagttt
     9241 ataatatcaa caacttgggc tacagaaaag aaaagttatt acagaaatta tgattaacaa
     9301 gtacatgtta aataaagatt ttaatatgaa tgccaccact ggagcattca tgccatttgg
     9361 agcttcttcc tgtttggatc actagaagga ggaggtcact cattacagtt ctcatataca
     9421 gtcgttggtt ggttggttgg ttggtagatt gattgattga ttgattgatt gattgattga
     9481 ttgattgatt gattgattga ttgattgatt gattgattga ttggtagtca aaataagaaa
     9541 taatttccac agattcatta cagaaatgat taaatgcata cataaaaaac tggggggggg
     9601 ggggatacaa caacacactt aagtcacatt tgcctacgta aagaaaagta aagaaaatca
     9661 atagctatat tttacatctc ctttttttgc tgtcttttaa ttagccttgt tttgctgtta
     9721 tcttgattga aactgtaact tcttcacctg cttcttttct ttgtaggttt tgccagccca
     9781 gaaacaggca tggctgtgca aggccagagc accatgcaca atgcgctgca tgtcttcatg
     9841 aacggctcaa tgtcctcagt ccagggctca gccaacgacc ccatcttcct ccttcaccat
     9901 gctttcattg acaggtaaca aacacgtcat gacattagac tgcacagttt ttgacaaagt
     9961 tcatacaatc tgttgtttat agctgctaca attagtgaag tttgtgaatg tacttggatg
    10021 agcagcgaaa gatcaattga gatcaattgt tagagtttgg ttgccctgca gagcaaagaa
    10081 caaaaaataa tctggtggct ttactgcgtg aggttattat tggtggaata gaaacacaaa
    10141 acataattgc atttatttgt ttaatttttt atcttatctt aactttcatc ttgcatattt
    10201 gtttcttaca tcatttctag catctttgag cgctggctaa gaactcatca gcctccccgg
    10261 tccatctacc cacgtaccaa tgcaccaatt ggccacaatg acggctacta catggtgcca
    10321 ttccttcctc tttataggaa tggagactac ctcctgtcca acaatgctct tggatacgag
    10381 tacgcctacc tgttggaccc aggtcattgc acaacaccag aaatgccctc tgatctgcaa
    10441 aagacgtgaa tatctgttca gacacccata tccactctgt tccacacagg tcagaggttt
    10501 gtccaggagt tcttgacaga ggtgtaaaaa gtactcaaaa attttactca agtgaaagta
    10561 caagtactta gggaaaattt tactcaatta aaagtaaaag tatctggcta gaatcttact
    10621 tgagtaaaag taaaaaagta ctccattaaa attgtacttg agtattaagg aagtaaaagt
    10681 aaaagcaaga aagaaaacta gagattcttg tttaagcttt taatctcaaa aaacattaaa
    10741 tgaaatgcat acaaggtttt atcctgcttt agaactgttt gtatttaatt atcaaactat
    10801 aagacagaca atctaatgcc agtacacgct actcaaagtt gtaaaacctc agatttaact
    10861 tcagtagaag ctgattctca aaattgttag tgtcaagcct agctcttttg gggctgaaaa
    10921 gcaatcctgc agtgctgaaa agcctctcac aggcagccga tgcgggaaga ggtgtattag
    10981 tcttgataga gaggctgcaa atagcaggaa acgtgagcag agactccctg gtgtctgaaa
    11041 cacaggccag atgggccctc gaggatatc
//
