LOCUS       sequence-175416-       10045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for reporter assay of one zebrafish super-enhancer region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10045)
  AUTHORS   Perez-Rico YA, Boeva V, Mallory AC, Bitetti A, Majello S, Barillot 
            E, Shkumatava A
  TITLE     Comparative analyses of super-enhancers reveal conserved elements in
            vertebrate genomes.
  JOURNAL   Genome Res. 2017 Feb;27(2):259-268. doi: 10.1101/gr.203679.115. Epub
            2016 Dec 13.
  PUBMED    27965291
REFERENCE   2  (bases 1 to 10045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.203679.115"; journalName: "Genome Res"; date: "2017-02";
            volume: "27"; issue: "2"; pages: "259-268"
COMMENT     Sequence Label: E1b-GFP-Tol2-Gateway dre SE-zic2a Q
FEATURES             Location/Qualifiers
     source          1..10045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..125
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(150..155,156..173,174..180)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             234..893
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(300..319)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1235..1540
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1419..1438
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1581..1705)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          2537..3109
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      3183..3192
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3189..3191,3192..3194,3195..3908)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3189..3191,3192..3194,3195..3905)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3234..3255)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3495..3514)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3842..3863
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3953..4087
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3977..3996)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4031..4050
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(4524..4711,4712..4723)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(4757..4773)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4757..4773)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4810..4830)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4810..4828)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4849..4865)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4849..4865)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4862..4884)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4873..4889
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4897..4903,4904..4921,4922..4927))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4942..4963
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5080..5097)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5251..5839)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5331..5350)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6010..6801,6802..6870))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6633..6652
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6871..6975)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7001..7456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7138..7159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7350..7369
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7583..7605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7597..7614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7598..7614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7624..7643
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7624..7642
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(9493..9504,9505..9667)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
       61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      121 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      181 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
      241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
      781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
      901 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      961 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1021 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1081 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1141 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1201 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     1261 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1321 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1381 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1441 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1501 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1561 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1681 gttcagcttt cttgtacaaa gtggtgatat caagatctgg tacaactgca ttgcaaaggg
     1741 tcagtggttg tgtgtgtgtg tgtgtgtgcg tgcgtgtgtg cgtgcgtgcg tgtgtgtgta
     1801 tttgttttgt ggagtaagag caaagtcccc catttgcagc ataagtgcca ggtaaatgca
     1861 tttgatatta tggcccttgc ctttgggggg gtcatcagcc cccttgccct attgtgccct
     1921 gtttaaaagg gtttgtcatg aatgtgagag agcctctttt cccctactgc tctcccctgg
     1981 aaatggagat gtaatgacat ttgcagctaa aaggagctca ttcacagttt gtacagttta
     2041 agaattcaaa tgaagtgttt tcgcgtgctg tattgcctca aggaacaggc gagagaggaa
     2101 agcagatttc tgaggattag ttctccaggt ttcctttatt atgttaacag tctatagtaa
     2161 atggtgatgg agggaaaact gaggtaaagt aaaggttgtg tgttggggag gggggcacgt
     2221 atcctcctca gcagggcctc aatggcatga taacagtgat actggacaca gtttacacac
     2281 acaaaaaatg ccagagttta cagcggtgag gttgttcaca gccattgaca gggaaacacc
     2341 tgttttcagg ggaaaaacag ctcctgtcaa aaccctctag ctcctgtctg gtttggtttt
     2401 agtaaataga ggcatgtgaa gggaaagtac gtggccagat ctctcgactc tagagggtat
     2461 ataatggatc ccatcgcgtc tcagcctcac tttgagctcc tccacacgaa tttcgaccga
     2521 tcctgagaac ttcagggtga gtttggggac ccttgattgt tctttctttt tcgctattgt
     2581 aaaattcatg ttatatggag ggggcaaagt tttcagggtg ttgtttagaa tgggaagatg
     2641 tcccttgtat caccatggac cctcatgata attttgtttc tttcactttc tactctgttg
     2701 acaaccattg tctcctctta ttttcttttc attttctgta actttttcgt taaactttag
     2761 cttgcatttg taacgaattt ttaaattcac ttttgtttat ttgtcagatt gtaagtactt
     2821 tctctaatca cttttttttc aaggcaatca gggtatatta tattgtactt cagcacagtt
     2881 ttagagaaca attgttataa ttaaatgata aggtagaata tttctgcata taaattctgg
     2941 ctggcgtgga aatattctta ttggtagaaa caactacacc ctggtcatca tcctgccttt
     3001 ctctttatgg ttacaatgat atacactgtt tgagatgagg ataaaatact ctgagtccaa
     3061 accgggcccc tctgctaacc atgttcatgc cttcttctct ttcctacagc tcctgggcaa
     3121 cgtgctggtt gttgtgctgt ctcatcattt tggcaaagaa ttcctcgacg gatccaccgg
     3181 tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     3241 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     3301 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     3361 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     3421 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     3481 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     3541 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     3601 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     3661 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     3721 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     3781 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     3841 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     3901 acaagtaaag cggccgccac cgcggtggag ctcgaattaa ttcatcgatg atgatccaga
     3961 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     4021 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     4081 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     4141 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga
     4201 tcagatccga agatacggcc acgggtgctc ttgatcctgt ggctgatttt ggactgtgct
     4261 gctcgcagct gctgatgaat cacatacttc ctccattttc ttccactgat tgactgttat
     4321 aatttcccta atttccaggt caaggtgctg tgcattgtgg taatagatgt gacatgacgt
     4381 cacttccaaa ggaccaatga acatgtctga ccaatttcat ataatgtgaa aacgattttc
     4441 ataggcagaa taaataacat ttaaattaaa ctgggcatca gcgcaattca attggtttgg
     4501 taatagcaag ggaaaataga atgaagtgat ctccaaaaaa taagtacttt ttgactgtaa
     4561 ataaaattgt aaggagtaaa aagtactttt ttttctaaaa aaatgtaatt aagtaaaagt
     4621 aaaagtattg atttttaatt gtactcaagt aaagtaaaaa tccccaaaaa taatacttaa
     4681 gtacagtaat caagtaaaat tactcaagta ctttacacct ctggttcttg accccctacc
     4741 ttcagcaagc ccagcagatc cactagttct agagcggccg ccaccgcggt ggagctccag
     4801 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     4861 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     4921 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     4981 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     5041 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     5101 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5161 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5221 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5281 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5341 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5401 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     5461 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     5521 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5581 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     5641 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     5701 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5761 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5821 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5881 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5941 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     6001 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6061 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6121 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6181 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6241 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6301 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6361 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6421 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     6481 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     6541 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     6601 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     6661 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     6721 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     6781 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     6841 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     6901 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     6961 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     7021 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     7081 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     7141 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     7201 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     7261 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     7321 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     7381 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     7441 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
     7501 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     7561 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
     7621 gcgtaatacg actcactata gggcgaattg ggtaccaaat agtaggaatt acccacctgt
     7681 acaagtgctg aaaacttgga tgaataagcc cgtttgccat tttctactgc tattttaaat
     7741 cttttctgta tttgtctgca tttgtcttta cccttgaata aatgttttag ttgttttttt
     7801 tcaattatga ctgtgtttaa cagacattat tacaataata tgtaatagta gtacacacta
     7861 ttattatgta atagtacttg ttgactgtat ttgagaactg gaccgagtga gtgttacgtc
     7921 acccattcaa aatgacttac ttctggctcc aacaaaatga agttaattca gttgccattt
     7981 ttcactgtat ggacatcgcc gtgttggagc tagacgttgc caataagcaa gaaagagccg
     8041 agatgcgtcg agtctgagtc accgttccta tggcaacccc tctaaccaat cagaagtaag
     8101 cttgttggaa gtccacagcc taccacttga aagcgggctg cacaaaatct gtcaaacgtt
     8161 ttgaacgttg gatgtgagag cacatacttt tattaaggca tctggttggt cagtttataa
     8221 tatcaacaac ttgggctaca gaaaagaaaa gttattacag aaattatgat taacaagtac
     8281 atgttaaata aagattttaa tatgaatgcc accactggag cattcatgcc atttggagct
     8341 tcttcctgtt tggatcacta gaaggaggag gtcactcatt acagttctca tatacagtcg
     8401 ttggttggtt ggttggttgg tagattgatt gattgattga ttgattgatt gattgattga
     8461 ttgattgatt gattgattga ttgattgatt gattgattgg tagtcaaaat aagaaataat
     8521 ttccacagat tcattacaga aatgattaaa tgcatacata aaaaactggg gggggggggg
     8581 atacaacaac acacttaagt cacatttgcc tacgtaaaga aaagtaaaga aaatcaatag
     8641 ctatatttta catctccttt ttttgctgtc ttttaattag ccttgttttg ctgttatctt
     8701 gattgaaact gtaacttctt cacctgcttc ttttctttgt aggttttgcc agcccagaaa
     8761 caggcatggc tgtgcaaggc cagagcacca tgcacaatgc gctgcatgtc ttcatgaacg
     8821 gctcaatgtc ctcagtccag ggctcagcca acgaccccat cttcctcctt caccatgctt
     8881 tcattgacag gtaacaaaca cgtcatgaca ttagactgca cagtttttga caaagttcat
     8941 acaatctgtt gtttatagct gctacaatta gtgaagtttg tgaatgtact tggatgagca
     9001 gcgaaagatc aattgagatc aattgttaga gtttggttgc cctgcagagc aaagaacaaa
     9061 aaataatctg gtggctttac tgcgtgaggt tattattggt ggaatagaaa cacaaaacat
     9121 aattgcattt atttgtttaa ttttttatct tatcttaact ttcatcttgc atatttgttt
     9181 cttacatcat ttctagcatc tttgagcgct ggctaagaac tcatcagcct ccccggtcca
     9241 tctacccacg taccaatgca ccaattggcc acaatgacgg ctactacatg gtgccattcc
     9301 ttcctcttta taggaatgga gactacctcc tgtccaacaa tgctcttgga tacgagtacg
     9361 cctacctgtt ggacccaggt cattgcacaa caccagaaat gccctctgat ctgcaaaaga
     9421 cgtgaatatc tgttcagaca cccatatcca ctctgttcca cacaggtcag aggtttgtcc
     9481 aggagttctt gacagaggtg taaaaagtac tcaaaaattt tactcaagtg aaagtacaag
     9541 tacttaggga aaattttact caattaaaag taaaagtatc tggctagaat cttacttgag
     9601 taaaagtaaa aaagtactcc attaaaattg tacttgagta ttaaggaagt aaaagtaaaa
     9661 gcaagaaaga aaactagaga ttcttgttta agcttttaat ctcaaaaaac attaaatgaa
     9721 atgcatacaa ggttttatcc tgctttagaa ctgtttgtat ttaattatca aactataaga
     9781 cagacaatct aatgccagta cacgctactc aaagttgtaa aacctcagat ttaacttcag
     9841 tagaagctga ttctcaaaat tgttagtgtc aagcctagct cttttggggc tgaaaagcaa
     9901 tcctgcagtg ctgaaaagcc tctcacaggc agccgatgcg ggaagaggtg tattagtctt
     9961 gatagagagg ctgcaaatag caggaaacgt gagcagagac tccctggtgt ctgaaacaca
    10021 ggccagatgg gccctcgagg atatc
//
