LOCUS       sequence-175420-       11078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for reporter assay of one zebrafish super-enhancer region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11078)
  AUTHORS   Perez-Rico YA, Boeva V, Mallory AC, Bitetti A, Majello S, Barillot 
            E, Shkumatava A
  TITLE     Comparative analyses of super-enhancers reveal conserved elements in
            vertebrate genomes.
  JOURNAL   Genome Res. 2017 Feb;27(2):259-268. doi: 10.1101/gr.203679.115. Epub
            2016 Dec 13.
  PUBMED    27965291
REFERENCE   2  (bases 1 to 11078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.203679.115"; journalName: "Genome Res"; date: "2017-02";
            volume: "27"; issue: "2"; pages: "259-268"
COMMENT     Sequence Label: E1b-GFP-Tol2-Gateway dre SE-zic2a M
FEATURES             Location/Qualifiers
     source          1..11078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..125
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(150..155,156..173,174..180)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             234..893
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(300..319)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1235..1540
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1419..1438
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1581..1705)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          3570..4142
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      4216..4225
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4222..4224,4225..4227,4228..4941)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4222..4224,4225..4227,4228..4938)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4267..4288)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4528..4547)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4875..4896
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4986..5120
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5010..5029)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5064..5083
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(5557..5744,5745..5756)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5790..5806)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5790..5806)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5843..5863)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5843..5861)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5882..5898)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5882..5898)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5895..5917)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5906..5922
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5930..5936,5937..5954,5955..5960))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5975..5996
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6113..6130)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6284..6872)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6364..6383)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7043..7834,7835..7903))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7666..7685
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7904..8008)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8034..8489)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8171..8192)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8383..8402
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8616..8638
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8630..8647
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8631..8647
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8657..8676
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8657..8675
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(10526..10537,10538..10700)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
       61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      121 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      181 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
      241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
      781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
      901 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      961 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1021 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1081 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1141 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1201 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     1261 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1321 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1381 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1441 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1501 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1561 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1681 gttcagcttt cttgtacaaa gtggtgatat caagatctgc tcttcaagcc ctgatctagt
     1741 ggaccacaaa gaacctcaaa aaaccaaaac taagaacccc aatggagaac tcaaaaattt
     1801 aaatgatttt ttcttcaatc aaaaatttat gtttttaatt actgatacca ttcaacatgg
     1861 atatttgttc acataaagtc tatatttcca ttgtaattgc agggaggaaa aggctctgca
     1921 ctgttaattc tagttatggt gagctataga ttatggaaat tattgtaagg aagaattaat
     1981 tatttaacaa aactagaaac aacaaaacca cagcaggggg caatgcagca caccagagag
     2041 aagggccgtt cttatgttaa gtgagattat tctgccttgc tgaaatatgg tatgaaaata
     2101 ccatatgaaa ttggctaacg cctccaaagc ctccatgcat actgactgca cagcaataag
     2161 attttattta gtcttcacaa ataaaaaaac taagaagcta agtgacaaca aaataaaaaa
     2221 atgctttctg aattctgagt gcattaatgt gtctcgcaga tttaatttgt aggcttaata
     2281 acgtaattaa cataattaat atttaatcca cacttcattg attttaaaat atgcatttca
     2341 aaaggatacc tcgcccaaaa ataattaaaa ttctgtaatt atttagcaac cttcatgcag
     2401 tttcaaacct tcatggaaaa cgacaatatt ttcaagaaga ttagtcatga aggtaaaata
     2461 taacattcta caatgtacca tttatataac aatatgttcc gttggggata aaatctaaac
     2521 ttttaaacta tttaataaca gctaaataat agctcaattt cttttattta atgaaacatc
     2581 cattcaattt attggacaca caagggtgta tcagctggat ttaacaatag aactcaggta
     2641 agccagattt ttagagctgc atttccatgt ctcaaaaaaa aaggctgtta attagggtca
     2701 tttatgttaa ttgtgtgctg gatcctaatg tcataccaat catacatgct cttccatcac
     2761 agctgtgttc tccacaaaag gctgaataac agacactgta aaacgtcctc tgcaaatatt
     2821 tataaagtct cacatcacag ctcagaattc aggcagagac atattttcct aatgcgtcca
     2881 aatatttcca cagtgcgcaa catttagtct attgatctct ggctcggtgg atttaaaaaa
     2941 tgtacgaatt gttggccaca tttcaaaagc atattctcag tcacatactg cttgtctttc
     3001 ccccccactt tgcttagact gcaaactcta gtcctaatga aggctttgcc acttgtaatt
     3061 gtttgattta tttcttgtca cattttaatt atgtactaaa ttagcaaaag ttgtactgca
     3121 tttgtatatt ttttcccttg attacgtcac tataatccac tggcagatgg atctcattat
     3181 catggctcaa acaaaatact gattagatta tcaaaaagtg acaattgttc tttaatagtg
     3241 ccagaaatat tttgtctaaa tcatacagat acaccagttg acacgtttat gtcagaatat
     3301 cagttttaat tgtgcaggaa attgtttaat caaatggggt tttcctctca ttagatttaa
     3361 accatcgtat tgaaaaatgc tgttgaatat ctgcaacatt tcatattctg actcatattg
     3421 ccttttgttt gaaaaagaaa aacatcaggt caatccttca catgccaaca gatctctcga
     3481 ctctagaggg tatataatgg atcccatcgc gtctcagcct cactttgagc tcctccacac
     3541 gaatttcgac cgatcctgag aacttcaggg tgagtttggg gacccttgat tgttctttct
     3601 ttttcgctat tgtaaaattc atgttatatg gagggggcaa agttttcagg gtgttgttta
     3661 gaatgggaag atgtcccttg tatcaccatg gaccctcatg ataattttgt ttctttcact
     3721 ttctactctg ttgacaacca ttgtctcctc ttattttctt ttcattttct gtaacttttt
     3781 cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag
     3841 attgtaagta ctttctctaa tcactttttt ttcaaggcaa tcagggtata ttatattgta
     3901 cttcagcaca gttttagaga acaattgtta taattaaatg ataaggtaga atatttctgc
     3961 atataaattc tggctggcgt ggaaatattc ttattggtag aaacaactac accctggtca
     4021 tcatcctgcc tttctcttta tggttacaat gatatacact gtttgagatg aggataaaat
     4081 actctgagtc caaaccgggc ccctctgcta accatgttca tgccttcttc tctttcctac
     4141 agctcctggg caacgtgctg gttgttgtgc tgtctcatca ttttggcaaa gaattcctcg
     4201 acggatccac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     4261 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     4321 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     4381 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     4441 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     4501 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     4561 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     4621 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     4681 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     4741 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     4801 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     4861 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     4921 atggacgagc tgtacaagta aagcggccgc caccgcggtg gagctcgaat taattcatcg
     4981 atgatgatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     5041 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     5101 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     5161 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     5221 atgatcctct agatcagatc cgaagatacg gccacgggtg ctcttgatcc tgtggctgat
     5281 tttggactgt gctgctcgca gctgctgatg aatcacatac ttcctccatt ttcttccact
     5341 gattgactgt tataatttcc ctaatttcca ggtcaaggtg ctgtgcattg tggtaataga
     5401 tgtgacatga cgtcacttcc aaaggaccaa tgaacatgtc tgaccaattt catataatgt
     5461 gaaaacgatt ttcataggca gaataaataa catttaaatt aaactgggca tcagcgcaat
     5521 tcaattggtt tggtaatagc aagggaaaat agaatgaagt gatctccaaa aaataagtac
     5581 tttttgactg taaataaaat tgtaaggagt aaaaagtact tttttttcta aaaaaatgta
     5641 attaagtaaa agtaaaagta ttgattttta attgtactca agtaaagtaa aaatccccaa
     5701 aaataatact taagtacagt aatcaagtaa aattactcaa gtactttaca cctctggttc
     5761 ttgaccccct accttcagca agcccagcag atccactagt tctagagcgg ccgccaccgc
     5821 ggtggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
     5881 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     5941 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6001 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6061 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6121 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6181 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6241 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6301 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6361 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6421 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6481 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6541 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6601 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6661 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6721 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6781 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     6841 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     6901 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     6961 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7021 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7081 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7141 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7201 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7261 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7321 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7381 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7441 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7501 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7561 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7621 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7681 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7741 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7801 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     7861 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     7921 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     7981 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
     8041 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     8101 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     8161 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     8221 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     8281 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     8341 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     8401 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     8461 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
     8521 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
     8581 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
     8641 ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttgggtacca aatagtagga
     8701 attacccacc tgtacaagtg ctgaaaactt ggatgaataa gcccgtttgc cattttctac
     8761 tgctatttta aatcttttct gtatttgtct gcatttgtct ttacccttga ataaatgttt
     8821 tagttgtttt ttttcaatta tgactgtgtt taacagacat tattacaata atatgtaata
     8881 gtagtacaca ctattattat gtaatagtac ttgttgactg tatttgagaa ctggaccgag
     8941 tgagtgttac gtcacccatt caaaatgact tacttctggc tccaacaaaa tgaagttaat
     9001 tcagttgcca tttttcactg tatggacatc gccgtgttgg agctagacgt tgccaataag
     9061 caagaaagag ccgagatgcg tcgagtctga gtcaccgttc ctatggcaac ccctctaacc
     9121 aatcagaagt aagcttgttg gaagtccaca gcctaccact tgaaagcggg ctgcacaaaa
     9181 tctgtcaaac gttttgaacg ttggatgtga gagcacatac ttttattaag gcatctggtt
     9241 ggtcagttta taatatcaac aacttgggct acagaaaaga aaagttatta cagaaattat
     9301 gattaacaag tacatgttaa ataaagattt taatatgaat gccaccactg gagcattcat
     9361 gccatttgga gcttcttcct gtttggatca ctagaaggag gaggtcactc attacagttc
     9421 tcatatacag tcgttggttg gttggttggt tggtagattg attgattgat tgattgattg
     9481 attgattgat tgattgattg attgattgat tgattgattg attgattgat tggtagtcaa
     9541 aataagaaat aatttccaca gattcattac agaaatgatt aaatgcatac ataaaaaact
     9601 nggggggggg gggatacaac aacacactta agtcacattt gcctacgtaa agaaaagtaa
     9661 agaaaatcaa tagctatatt ttacatctcc tttttttgct gtcttttaat tagccttgtt
     9721 ttgctgttat cttgattgaa actgtaactt cttcacctgc ttcttttctt tgtaggtttt
     9781 gccagcccag aaacaggcat ggctgtgcaa ggccagagca ccatgcacaa tgcgctgcat
     9841 gtcttcatga acggctcaat gtcctcagtc cagggctcag ccaacgaccc catcttcctc
     9901 cttcaccatg ctttcattga caggtaacaa acacgtcatg acattagact gcacagtttt
     9961 tgacaaagtt catacaatct gttgtttata gctgctacaa ttagtgaagt ttgtgaatgt
    10021 acttggatga gcagcgaaag atcaattgag atcaattgtt agagtttggt tgccctgcag
    10081 agcaaagaac aaaaaataat ctggtggctt tactgcgtga ggttattatt ggtggaatag
    10141 aaacacaaaa cataattgca tttatttgtt taatttttta tcttatctta actttcatct
    10201 tgcatatttg tttcttacat catttctagc atctttgagc gctggctaag aactcatcag
    10261 cctccccggt ccatctaccc acgtaccaat gcaccaattg gccacaatga cggctactac
    10321 atggtgccat tccttcctct ttataggaat ggagactacc tcctgtccaa caatgctctt
    10381 ggatacgagt acgcctacct gttggaccca ggtcattgca caacaccaga aatgccctct
    10441 gatctgcaaa agacgtgaat atctgttcag acacccatat ccactctgtt ccacacaggt
    10501 cagaggtttg tccaggagtt cttgacagag gtgtaaaaag tactcaaaaa ttttactcaa
    10561 gtgaaagtac aagtacttag ggaaaatttt actcaattaa aagtaaaagt atctggctag
    10621 aatcttactt gagtaaaagt aaaaaagtac tccattaaaa ttgtacttga gtattaagga
    10681 agtaaaagta aaagcaagaa agaaaactag agattcttgt ttaagctttt aatctcaaaa
    10741 aacattaaat gaaatgcata caaggtttta tcctgcttta gaactgtttg tatttaatta
    10801 tcaaactata agacagacaa tctaatgcca gtacacgcta ctcaaagttg taaaacctca
    10861 gatttaactt cagtagaagc tgattctcaa aattgttagt gtcaagccta gctcttttgg
    10921 ggctgaaaag caatcctgca gtgctgaaaa gcctctcaca ggcagccgat gcgggaagag
    10981 gtgtattagt cttgatagag aggctgcaaa tagcaggaaa cgtgagcaga gactccctgg
    11041 tgtctgaaac acaggccaga tgggccctcg aggatatc
//
