LOCUS       sequence-175421-       10934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for reporter assay of one zebrafish super-enhancer region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10934)
  AUTHORS   Perez-Rico YA, Boeva V, Mallory AC, Bitetti A, Majello S, Barillot 
            E, Shkumatava A
  TITLE     Comparative analyses of super-enhancers reveal conserved elements in
            vertebrate genomes.
  JOURNAL   Genome Res. 2017 Feb;27(2):259-268. doi: 10.1101/gr.203679.115. Epub
            2016 Dec 13.
  PUBMED    27965291
REFERENCE   2  (bases 1 to 10934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.203679.115"; journalName: "Genome Res"; date: "2017-02";
            volume: "27"; issue: "2"; pages: "259-268"
COMMENT     Sequence Label: E1b-GFP-Tol2-Gateway dre SE-zic2a P
FEATURES             Location/Qualifiers
     source          1..10934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..125
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(150..155,156..173,174..180)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             234..893
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(300..319)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1235..1540
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1419..1438
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1581..1705)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          3426..3998
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      4072..4081
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4078..4080,4081..4083,4084..4797)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4078..4080,4081..4083,4084..4794)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4123..4144)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4384..4403)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4731..4752
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4842..4976
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4866..4885)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4920..4939
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(5413..5600,5601..5612)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5646..5662)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5646..5662)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5699..5719)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5699..5717)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5738..5754)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5738..5754)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5751..5773)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5762..5778
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5786..5792,5793..5810,5811..5816))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5831..5852
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5969..5986)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6140..6728)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6220..6239)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6899..7690,7691..7759))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7522..7541
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7760..7864)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7890..8345)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8027..8048)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8239..8258
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8472..8494
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8486..8503
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8487..8503
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8513..8532
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8513..8531
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(10382..10393,10394..10556)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
       61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      121 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      181 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
      241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
      781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
      901 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      961 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1021 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1081 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1141 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1201 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     1261 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1321 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1381 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1441 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1501 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1561 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1681 gttcagcttt cttgtacaaa gtggtgatat caagatctca ccgctggtct tgacatgtta
     1741 aaaaggccat tttcatcttt tgtcccgaga aaagcaggag gaaaggactc tctgtagctg
     1801 taaataactg taaacatcca ggtaatagag gctcttttgt gtttcttggc catggttgtt
     1861 accttgttat ctctagcttt tatgttaata atcagacact caccatcttt gccaactgcg
     1921 tgggttacct ttttgtatgc gcaatcgcta ttttgtcgag cttgttgaaa agggggtgaa
     1981 tctctccccc caaaaaaggg agggctatct ttgccctgtt acctgagggg attttacccc
     2041 accctgccca attttcacct acctctgttt actcacagtc ctcactgtat gtcacaacat
     2101 ttacatgtct ttccttttgc ttcaatttgt atgtgttaga tagaaaaaaa attgagcaat
     2161 ggaaaaaaat ccctaataga agagcagtcc cctgcaactg tgaaaacccc tccgctggtc
     2221 agccaatggc cattcacggg agaacaaaca aaaatcaaat gggagctatg cttttagctt
     2281 ttaattccag ccccaaaagt ttccagctgc ttccgtaatt attacctttt aaagagtagg
     2341 aaaatggcgg gttaattcac atggtggctt tctctatctt tttgtctttt tctcccttac
     2401 tccctgcctc attctctctt tcctcctctc tccaccccaa aaccacaaca ggtaagctct
     2461 actgttaaac gagggccttt tttagttgta atttggtttg aaaaccctct ggcttgccca
     2521 gattattctt agtcgtttct ctgtttcctg tcggtatgaa ggaggtgcac cttgtgctca
     2581 gggagaggaa caatacagcc ttgtcccagg ttcctctgac cccctgcgct gcctgtcagt
     2641 ctgtgattgt catgcaaggc cctctctaga aacaaaaggc ctagatgggc tttgtggccc
     2701 accaggaccc cagcattgtt attgggatcc ctgtgagtat ttgctcttga gtctgtttct
     2761 caacctgaaa gaccttgatg ctgatggttt tgttaatcta agatgtaacg tttaaggtta
     2821 tcttgtttta gttgtggggt tgatttacgt caacatcgtt ataagccata caaaaagtaa
     2881 gtcattttaa tatatttagc atgtgattca ggtggatagt atgtatctgc attgccttgt
     2941 tactgttact gtagccactg catatcagtg tgaattaaag gtttggggtc agtacatgta
     3001 catgaggaga cgtagccctg cgctattgtt aaagctcggg tgatggatgg ctgtggtcgc
     3061 ccatgatccc agcccctgct ggataggcta tggaaaaact cagcttcata ttgtccttgt
     3121 tctgtgtgac aatgggccac ttgtgggtct gacactcatc acctgttagt cggcctcatt
     3181 cgtatgaaaa taccacgctg ccttggttcc agcactgtag gtgctttccc tccagccttg
     3241 tatgtttgtt tagacaatag tgcccatcac aaagtgacac ttgtaattga gagcttttca
     3301 tatcaagctg aggtggatgt gtttcagatc tctcgactct agagggtata taatggatcc
     3361 catcgcgtct cagcctcact ttgagctcct ccacacgaat ttcgaccgat cctgagaact
     3421 tcagggtgag tttggggacc cttgattgtt ctttcttttt cgctattgta aaattcatgt
     3481 tatatggagg gggcaaagtt ttcagggtgt tgtttagaat gggaagatgt cccttgtatc
     3541 accatggacc ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt
     3601 ctcctcttat tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt
     3661 aacgaatttt taaattcact tttgtttatt tgtcagattg taagtacttt ctctaatcac
     3721 ttttttttca aggcaatcag ggtatattat attgtacttc agcacagttt tagagaacaa
     3781 ttgttataat taaatgataa ggtagaatat ttctgcatat aaattctggc tggcgtggaa
     3841 atattcttat tggtagaaac aactacaccc tggtcatcat cctgcctttc tctttatggt
     3901 tacaatgata tacactgttt gagatgagga taaaatactc tgagtccaaa ccgggcccct
     3961 ctgctaacca tgttcatgcc ttcttctctt tcctacagct cctgggcaac gtgctggttg
     4021 ttgtgctgtc tcatcatttt ggcaaagaat tcctcgacgg atccaccggt cgccaccatg
     4081 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     4141 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     4201 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     4261 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     4321 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     4381 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     4441 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     4501 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     4561 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     4621 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     4681 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     4741 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaaagc
     4801 ggccgccacc gcggtggagc tcgaattaat tcatcgatga tgatccagac atgataagat
     4861 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     4921 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     4981 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     5041 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagat cagatccgaa
     5101 gatacggcca cgggtgctct tgatcctgtg gctgattttg gactgtgctg ctcgcagctg
     5161 ctgatgaatc acatacttcc tccattttct tccactgatt gactgttata atttccctaa
     5221 tttccaggtc aaggtgctgt gcattgtggt aatagatgtg acatgacgtc acttccaaag
     5281 gaccaatgaa catgtctgac caatttcata taatgtgaaa acgattttca taggcagaat
     5341 aaataacatt taaattaaac tgggcatcag cgcaattcaa ttggtttggt aatagcaagg
     5401 gaaaatagaa tgaagtgatc tccaaaaaat aagtactttt tgactgtaaa taaaattgta
     5461 aggagtaaaa agtacttttt tttctaaaaa aatgtaatta agtaaaagta aaagtattga
     5521 tttttaattg tactcaagta aagtaaaaat ccccaaaaat aatacttaag tacagtaatc
     5581 aagtaaaatt actcaagtac tttacacctc tggttcttga ccccctacct tcagcaagcc
     5641 cagcagatcc actagttcta gagcggccgc caccgcggtg gagctccagc ttttgttccc
     5701 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     5761 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     5821 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     5881 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     5941 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     6001 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     6061 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     6121 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     6181 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     6241 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     6301 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     6361 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     6421 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     6481 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     6541 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     6601 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     6661 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     6721 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     6781 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     6841 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     6901 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     6961 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7021 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7081 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7141 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7201 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7261 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7321 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7381 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7441 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7501 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7561 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7621 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7681 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     7741 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     7801 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     7861 cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt
     7921 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     7981 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     8041 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     8101 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     8161 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     8221 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     8281 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     8341 cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     8401 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     8461 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga
     8521 ctcactatag ggcgaattgg gtaccaaata gtaggaatta cccacctgta caagtgctga
     8581 aaacttggat gaataagccc gtttgccatt ttctactgct attttaaatc ttttctgtat
     8641 ttgtctgcat ttgtctttac ccttgaataa atgttttagt tgtttttttt caattatgac
     8701 tgtgtttaac agacattatt acaataatat gtaatagtag tacacactat tattatgtaa
     8761 tagtacttgt tgactgtatt tgagaactgg accgagtgag tgttacgtca cccattcaaa
     8821 atgacttact tctggctcca acaaaatgaa gttaattcag ttgccatttt tcactgtatg
     8881 gacatcgccg tgttggagct agacgttgcc aataagcaag aaagagccga gatgcgtcga
     8941 gtctgagtca ccgttcctat ggcaacccct ctaaccaatc agaagtaagc ttgttggaag
     9001 tccacagcct accacttgaa agcgggctgc acaaaatctg tcaaacgttt tgaacgttgg
     9061 atgtgagagc acatactttt attaaggcat ctggttggtc agtttataat atcaacaact
     9121 tgggctacag aaaagaaaag ttattacaga aattatgatt aacaagtaca tgttaaataa
     9181 agattttaat atgaatgcca ccactggagc attcatgcca tttggagctt cttcctgttt
     9241 ggatcactag aaggaggagg tcactcatta cagttctcat atacagtcgt tggttggttg
     9301 gttggttggt agattgattg attgattgat tgattgattg attgattgat tgattgattg
     9361 attgattgat tgattgattg attgattggt agtcaaaata agaaataatt tccacagatt
     9421 cattacagaa atgattaaat gcatacataa aaaactgggg ggggggggga tacaacaaca
     9481 cacttaagtc acatttgcct acgtaaagaa aagtaaagaa aatcaatagc tatattttac
     9541 atctcctttt tttgctgtct tttaattagc cttgttttgc tgttatcttg attgaaactg
     9601 taacttcttc acctgcttct tttctttgta ggttttgcca gcccagaaac aggcatggct
     9661 gtgcaaggcc agagcaccat gcacaatgcg ctgcatgtct tcatgaacgg ctcaatgtcc
     9721 tcagtccagg gctcagccaa cgaccccatc ttcctccttc accatgcttt cattgacagg
     9781 taacaaacac gtcatgacat tagactgcac agtttttgac aaagttcata caatctgttg
     9841 tttatagctg ctacaattag tgaagtttgt gaatgtactt ggatgagcag cgaaagatca
     9901 attgagatca attgttagag tttggttgcc ctgcagagca aagaacaaaa aataatctgg
     9961 tggctttact gcgtgaggtt attattggtg gaatagaaac acaaaacata attgcattta
    10021 tttgtttaat tttttatctt atcttaactt tcatcttgca tatttgtttc ttacatcatt
    10081 tctagcatct ttgagcgctg gctaagaact catcagcctc cccggtccat ctacccacgt
    10141 accaatgcac caattggcca caatgacggc tactacatgg tgccattcct tcctctttat
    10201 aggaatggag actacctcct gtccaacaat gctcttggat acgagtacgc ctacctgttg
    10261 gacccaggtc attgcacaac accagaaatg ccctctgatc tgcaaaagac gtgaatatct
    10321 gttcagacac ccatatccac tctgttccac acaggtcaga ggtttgtcca ggagttcttg
    10381 acagaggtgt aaaaagtact caaaaatttt actcaagtga aagtacaagt acttagggaa
    10441 aattttactc aattaaaagt aaaagtatct ggctagaatc ttacttgagt aaaagtaaaa
    10501 aagtactcca ttaaaattgt acttgagtat taaggaagta aaagtaaaag caagaaagaa
    10561 aactagagat tcttgtttaa gcttttaatc tcaaaaaaca ttaaatgaaa tgcatacaag
    10621 gttttatcct gctttagaac tgtttgtatt taattatcaa actataagac agacaatcta
    10681 atgccagtac acgctactca aagttgtaaa acctcagatt taacttcagt agaagctgat
    10741 tctcaaaatt gttagtgtca agcctagctc ttttggggct gaaaagcaat cctgcagtgc
    10801 tgaaaagcct ctcacaggca gccgatgcgg gaagaggtgt attagtcttg atagagaggc
    10861 tgcaaatagc aggaaacgtg agcagagact ccctggtgtc tgaaacacag gccagatggg
    10921 ccctcgagga tatc
//
