LOCUS       sequence-175423-       10999 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for reporter assay of one mouse super-enhancer region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10999)
  AUTHORS   Perez-Rico YA, Boeva V, Mallory AC, Bitetti A, Majello S, Barillot 
            E, Shkumatava A
  TITLE     Comparative analyses of super-enhancers reveal conserved elements in
            vertebrate genomes.
  JOURNAL   Genome Res. 2017 Feb;27(2):259-268. doi: 10.1101/gr.203679.115. Epub
            2016 Dec 13.
  PUBMED    27965291
REFERENCE   2  (bases 1 to 10999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.203679.115"; journalName: "Genome Res"; date: "2017-02";
            volume: "27"; issue: "2"; pages: "259-268"
COMMENT     Sequence Label: E1b-GFP-Tol2-Gateway mmu SE-Irf2bpl I
FEATURES             Location/Qualifiers
     source          1..10999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..125
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(150..155,156..173,174..180)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             234..893
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(300..319)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1235..1540
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1419..1438
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1581..1705)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          3491..4063
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      4137..4146
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4143..4145,4146..4148,4149..4862)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4143..4145,4146..4148,4149..4859)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4188..4209)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4449..4468)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4796..4817
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4907..5041
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4931..4950)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4985..5004
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(5478..5665,5666..5677)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5711..5727)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5711..5727)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5764..5784)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5764..5782)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5803..5819)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5803..5819)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5816..5838)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5827..5843
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5851..5857,5858..5875,5876..5881))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5896..5917
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6034..6051)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6205..6793)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6285..6304)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6964..7755,7756..7824))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7587..7606
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7825..7929)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7955..8410)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8092..8113)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8304..8323
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8537..8559
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8551..8568
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8552..8568
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8578..8597
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8578..8596
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(10447..10458,10459..10621)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
       61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      121 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      181 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
      241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
      781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
      901 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      961 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1021 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1081 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1141 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1201 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     1261 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1321 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1381 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1441 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1501 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1561 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1681 gttcagcttt cttgtacaaa gtggtgatat caagatctca gtctctagct caattttgcc
     1741 tcagtttcct ctctgctgac taaaaaggga ttttgtgagg atcaaatgac ctcacaactg
     1801 ggcagcaggg tcccagactt cactcagtgt ctttcagtgc aaggatgact ccagtgttgt
     1861 gggcgtctct caggctgtga gactttctca ctgaggacag ttctgaagct agcagcccct
     1921 gtgagaggca accagcatgt gggcaaatcc tcagcacaca cgttctttcc cttttcctca
     1981 agggtggtgt tttcctcaga caacagccag cttcctgcct ttggcctgtt attcagaggt
     2041 accgctcaca aggcgtggta ctcagggtac tggagcttct ccacggaggg ctagcaagga
     2101 gagagaggtg ctccaccgtg gtggggatca ctccagggga ggaattggaa accacagcag
     2161 aaggtccgag agaacaaaga catgaaggat cccacccaat tctttcctac agcgaccttc
     2221 caaaaagggt cgctctttct gggtggtgac ctgaaaggta cctcccactg gaattatcta
     2281 ggatggcccg ccccatctgc ctatttctct ggggttcact accaccttgc tggggaagct
     2341 tgttgttcct cctgggaaca gggtttctct gtgtagcccg ggcttgtcct agcctccagc
     2401 gggcactgag cctccaccct cccctctcct tcctggacac gtggttttat ttttaacctg
     2461 aatttacagg caggatctgg aaaagccagt ttgtggcagc ttctagtctt tctgcttccc
     2521 tcccccacct gaagagtaag tagctggacc gtgcttggag aggtaggggg gcctgggcag
     2581 caggtgtgga taaagggcaa cctgctaggc cttgccagtt agaggactcc cagctctagt
     2641 ggggtggggt gaaatcagcc gaaccttctg ggcttttctg atgtaaccac cctagcagag
     2701 gctgattggc ccagggcctc catgtatcac aggaaggctc tgggcagagt ggcccaatgc
     2761 cctggtattt caggccagcc tgtctctggc actagagata gggcaggcaa ggtcaggctg
     2821 cccaagaggc agaggacaca cagatgtatg gtactggcag agagaaggta tcagagcatt
     2881 ttgatttcct ttggtttttt aaaattgggt gttttatttt aattttttta aattaattta
     2941 tttgtttgtt tgtttttggt ttttcaagac agggtttctc tgtatagccc tggctgacct
     3001 ggaactcatt ctgtagacca agctggcctc aaactcagaa gtccacctgc ctctgcctcc
     3061 cgagtgctgg gattaaaggc gtgcactccc atgcccagct gtgttttatt tttaattacg
     3121 tgtatgtgtg tatgcctttg tgtaggggtg gggggtgggg gtgtccatat gagtgcaggt
     3181 gacttcagag gccaggggtg tgggttccct tggagctgga gttataggca gttgtgagcc
     3241 acctgatatg gatctgatat ggatgctggg aactgaacca gggtcctctg gaacaggagc
     3301 aatgtctctt accctctgaa ccatctcact ccaggtccct cccccccccc tttttttttt
     3361 cctttttggt aggcaaactc ttggtgtttc agatctctcg actctagagg gtatataatg
     3421 gatcccatcg cgtctcagcc tcactttgag ctcctccaca cgaatttcga ccgatcctga
     3481 gaacttcagg gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt
     3541 catgttatat ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt
     3601 gtatcaccat ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc
     3661 attgtctcct cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca
     3721 tttgtaacga atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta
     3781 atcacttttt tttcaaggca atcagggtat attatattgt acttcagcac agttttagag
     3841 aacaattgtt ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg
     3901 tggaaatatt cttattggta gaaacaacta caccctggtc atcatcctgc ctttctcttt
     3961 atggttacaa tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg
     4021 cccctctgct aaccatgttc atgccttctt ctctttccta cagctcctgg gcaacgtgct
     4081 ggttgttgtg ctgtctcatc attttggcaa agaattcctc gacggatcca ccggtcgcca
     4141 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     4201 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     4261 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     4321 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     4381 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     4441 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     4501 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     4561 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     4621 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     4681 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     4741 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     4801 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     4861 aaagcggccg ccaccgcggt ggagctcgaa ttaattcatc gatgatgatc cagacatgat
     4921 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     4981 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     5041 taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt
     5101 ttaaagcaag taaaacctct acaaatgtgg tatggctgat tatgatcctc tagatcagat
     5161 ccgaagatac ggccacgggt gctcttgatc ctgtggctga ttttggactg tgctgctcgc
     5221 agctgctgat gaatcacata cttcctccat tttcttccac tgattgactg ttataatttc
     5281 cctaatttcc aggtcaaggt gctgtgcatt gtggtaatag atgtgacatg acgtcacttc
     5341 caaaggacca atgaacatgt ctgaccaatt tcatataatg tgaaaacgat tttcataggc
     5401 agaataaata acatttaaat taaactgggc atcagcgcaa ttcaattggt ttggtaatag
     5461 caagggaaaa tagaatgaag tgatctccaa aaaataagta ctttttgact gtaaataaaa
     5521 ttgtaaggag taaaaagtac ttttttttct aaaaaaatgt aattaagtaa aagtaaaagt
     5581 attgattttt aattgtactc aagtaaagta aaaatcccca aaaataatac ttaagtacag
     5641 taatcaagta aaattactca agtactttac acctctggtt cttgaccccc taccttcagc
     5701 aagcccagca gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg
     5761 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     5821 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     5881 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     5941 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6001 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6061 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6121 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     6181 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     6241 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     6301 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     6361 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     6421 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     6481 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     6541 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     6601 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     6661 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     6721 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     6781 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     6841 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     6901 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6961 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     7021 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     7081 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     7141 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     7201 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     7261 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     7321 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     7381 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     7441 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     7501 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     7561 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     7621 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7681 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7741 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7801 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7861 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7921 ggttccgcgc acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta
     7981 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     8041 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     8101 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     8161 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     8221 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     8281 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     8341 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     8401 cagggcgcgt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     8461 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     8521 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa
     8581 tacgactcac tatagggcga attgggtacc aaatagtagg aattacccac ctgtacaagt
     8641 gctgaaaact tggatgaata agcccgtttg ccattttcta ctgctatttt aaatcttttc
     8701 tgtatttgtc tgcatttgtc tttacccttg aataaatgtt ttagttgttt tttttcaatt
     8761 atgactgtgt ttaacagaca ttattacaat aatatgtaat agtagtacac actattatta
     8821 tgtaatagta cttgttgact gtatttgaga actggaccga gtgagtgtta cgtcacccat
     8881 tcaaaatgac ttacttctgg ctccaacaaa atgaagttaa ttcagttgcc atttttcact
     8941 gtatggacat cgccgtgttg gagctagacg ttgccaataa gcaagaaaga gccgagatgc
     9001 gtcgagtctg agtcaccgtt cctatggcaa cccctctaac caatcagaag taagcttgtt
     9061 ggaagtccac agcctaccac ttgaaagcgg gctgcacaaa atctgtcaaa cgttttgaac
     9121 gttggatgtg agagcacata cttttattaa ggcatctggt tggtcagttt ataatatcaa
     9181 caacttgggc tacagaaaag aaaagttatt acagaaatta tgattaacaa gtacatgtta
     9241 aataaagatt ttaatatgaa tgccaccact ggagcattca tgccatttgg agcttcttcc
     9301 tgtttggatc actagaagga ggaggtcact cattacagtt ctcatataca gtcgttggtt
     9361 ggttggttgg ttggtagatt gattgattga ttgattgatt gattgattga ttgattgatt
     9421 gattgattga ttgattgatt gattgattga ttggtagtca aaataagaaa taatttccac
     9481 agattcatta cagaaatgat taaatgcata cataaaaaac tggggggggg ggggatacaa
     9541 caacacactt aagtcacatt tgcctacgta aagaaaagta aagaaaatca atagctatat
     9601 tttacatctc ctttttttgc tgtcttttaa ttagccttgt tttgctgtta tcttgattga
     9661 aactgtaact tcttcacctg cttcttttct ttgtaggttt tgccagccca gaaacaggca
     9721 tggctgtgca aggccagagc accatgcaca atgcgctgca tgtcttcatg aacggctcaa
     9781 tgtcctcagt ccagggctca gccaacgacc ccatcttcct ccttcaccat gctttcattg
     9841 acaggtaaca aacacgtcat gacattagac tgcacagttt ttgacaaagt tcatacaatc
     9901 tgttgtttat agctgctaca attagtgaag tttgtgaatg tacttggatg agcagcgaaa
     9961 gatcaattga gatcaattgt tagagtttgg ttgccctgca gagcaaagaa caaaaaataa
    10021 tctggtggct ttactgcgtg aggttattat tggtggaata gaaacacaaa acataattgc
    10081 atttatttgt ttaatttttt atcttatctt aactttcatc ttgcatattt gtttcttaca
    10141 tcatttctag catctttgag cgctggctaa gaactcatca gcctccccgg tccatctacc
    10201 cacgtaccaa tgcaccaatt ggccacaatg acggctacta catggtgcca ttccttcctc
    10261 tttataggaa tggagactac ctcctgtcca acaatgctct tggatacgag tacgcctacc
    10321 tgttggaccc aggtcattgc acaacaccag aaatgccctc tgatctgcaa aagacgtgaa
    10381 tatctgttca gacacccata tccactctgt tccacacagg tcagaggttt gtccaggagt
    10441 tcttgacaga ggtgtaaaaa gtactcaaaa attttactca agtgaaagta caagtactta
    10501 gggaaaattt tactcaatta aaagtaaaag tatctggcta gaatcttact tgagtaaaag
    10561 taaaaaagta ctccattaaa attgtacttg agtattaagg aagtaaaagt aaaagcaaga
    10621 aagaaaacta gagattcttg tttaagcttt taatctcaaa aaacattaaa tgaaatgcat
    10681 acaaggtttt atcctgcttt agaactgttt gtatttaatt atcaaactat aagacagaca
    10741 atctaatgcc agtacacgct actcaaagtt gtaaaacctc agatttaact tcagtagaag
    10801 ctgattctca aaattgttag tgtcaagcct agctcttttg gggctgaaaa gcaatcctgc
    10861 agtgctgaaa agcctctcac aggcagccga tgcgggaaga ggtgtattag tcttgataga
    10921 gaggctgcaa atagcaggaa acgtgagcag agactccctg gtgtctgaaa cacaggccag
    10981 atgggccctc gaggatatc
//
