LOCUS       sequence-175424-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Shuttle and expression vector. Replicates in E.coli and 
            Mycobacterium. Gives acetamide-inducible gene expression in 
            Mycobacterium. Copy number is controllable by arabinose in E.coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Ly MA, Liew EF, Le NB, Coleman NV
  TITLE     Construction and evaluation of pMycoFos, a fosmid shuttle vector for
            Mycobacterium spp. with inducible gene expression and copy number 
            control.
  JOURNAL   J Microbiol Methods. 2011 Sep;86(3):320-6. doi: 
            10.1016/j.mimet.2011.06.005. Epub 2011 Jun 13.
  PUBMED    21689690
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.mimet.2011.06.005"; journalName: "J Microbiol Methods"; 
            date: "2011-09"; volume: "86"; issue: "3"; pages: "320-6"
COMMENT     Sequence Label: pMycoFos
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    46..519
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 ctgtctttac ttaatgtcct ttgttacagg ccagaaagca taactggcct gaatattctc
       61 tctgggccca ctgttccact tgtatcgtcg gtctgataat cagactggga ccacggtccc
      121 actcgtatcg tcggtctgat tattagtctg ggaccacggt cccactcgta tcgtcggtct
      181 gattattagt ctgggaccac ggtcccactc gtatcgtcgg tctgataatc agactgggac
      241 cacggtccca ctcgtatcgt cggtctgatt attagtctgg gaccatggtc ccactcgtat
      301 cgtcggtctg attattagtc tgggaccacg gtcccactcg tatcgtcggt ctgattatta
      361 gtctggaacc acggtcccac tcgtatcgtc ggtctgatta ttagtctggg accacggtcc
      421 cactcgtatc gtcggtctga ttattagtct gggaccacga tcccactcgt gttgtcggtc
      481 tgattatcgg tctgggacca cggtcccact tgtattgtcg atcagactat cagcgtgaga
      541 ctacgattcc atcaatgcct gtcaagggca agtattgaca tgtcgtcgta acctgtagaa
      601 cggagtaacc tcggtgtgcg gttgtatgcc tgctgtggat tgctgctgtg tcctgcttat
      661 ccacaacatt ttgcgcacgg ttatgtggac aaaatacctg gttacccagg ccgtgccggc
      721 acgttaaccg ggctgcatcc gatgcaagtg tgtcgctgtc gac
//
