LOCUS       sequence-175425-       12518 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Shuttle and expression vector. Replicates in E.coli and 
            Mycobacterium. Gives acetamide-inducible gene expression in 
            Mycobacterium. Copy number is controllable by arabinose in E.coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12518)
  AUTHORS   Ly MA, Liew EF, Le NB, Coleman NV
  TITLE     Construction and evaluation of pMycoFos, a fosmid shuttle vector for
            Mycobacterium spp. with inducible gene expression and copy number 
            control.
  JOURNAL   J Microbiol Methods. 2011 Sep;86(3):320-6. doi: 
            10.1016/j.mimet.2011.06.005. Epub 2011 Jun 13.
  PUBMED    21689690
REFERENCE   2  (bases 1 to 12518)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.mimet.2011.06.005"; journalName: "J Microbiol Methods"; 
            date: "2011-09"; volume: "86"; issue: "3"; pages: "320-6"
COMMENT     Sequence Label: pMycoFos
FEATURES             Location/Qualifiers
     source          1..12518
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4781..4800)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             4792..5607
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(4865..4884)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      6489..7103
                     /label=oriV
                     /note="origin of replication for the bacterial F plasmid"
     rep_origin      6580..7111
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      7179..7398
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             7489..8244
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    8247..8497
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             8823..9998
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             9998..10969
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     misc_feature    11042..11515
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
     misc_feature    11775..12173
                     /label=cos
                     /note="lambda cos site; allows packaging into phage lambda 
                     particles"
     protein_bind    complement(12191..12224)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     12292..12311
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tcgatctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
       61 cagccgaacg accgagcgca acgcgtgagc ccaccagctc cgtaagttcg ggcgctgtgt
      121 ggctcgtacc cgcgcattca ggcggcaggg ggtctaacgg gtctaaggcg gcgtgtacgg
      181 ccgccacagc ggctctcagc ggcccggaaa cgtcctcgaa acgacgcatg tgttcctcct
      241 ggttggtaca ggtggttggg ggtgctcggc tgtcgctggt gttccaccac cagggctcga
      301 cgggagagcg ggggagtgtg cagttgtggg gtggcccctc agcgaaatat ctgacttgga
      361 gctcgtgtcg gaccatacac cggtgattaa tcgtggtcta ctaccaagcg tgagccacgt
      421 cgccgacgaa tttgagcagc tctggctgcc gtactggccg ctggcaagcg acgatctgct
      481 cgaggggatc taccgccaaa gccgcgcgtc ggccctaggc cgccggtaca tcgaggcgaa
      541 cccaacagcg ctggcaaacc tgctggtcgt ggacgtagac catccagacg cagcgctccg
      601 agcgctcagc gcccgggggt cccatccgct gcccaacgcg atcgtgggca atcgcgccaa
      661 cggccacgca cacgcagtgt gggcactcaa cgcccctgtt ccacgcaccg aatacgcgcg
      721 gcgtaagccg ctcgcataca tggcggcgtg cgccgaaggc cttcggcgcg ccgtcgatgg
      781 cgaccgcagt tactcaggcc tcatgaccaa aaaccccggc cacatcgcct gggaaacgga
      841 atggctccac tcagatctct acacactcag ccacatcgag gccgagctcg gcgcgaacat
      901 gccaccgccg cgctggcgtc agcagaccac gtacaaagcg gctccgacgc cgctagggcg
      961 gaattgcgca ctgttcgatt ccgtcaggtt gtgggcctat cgtcccgccc tcatgcggat
     1021 ctacctgccg acccggaacg tggacggact cggccgcgcg atctatgccg agtgccacgc
     1081 gcgaaacgcc gaatttccgt gcaacgacgt gtgtcccgga ccgctaccgg acagcgaggt
     1141 ccgcgccatc gccaacagca tttggcgttg gatcacaacc aagtcgcgca tttgggcgga
     1201 cgggatcgtg gtctacgagg ccacactcag tgcgcgccag tcggccatct cgcggaaggg
     1261 cgcagcagcg cgcacggcgg cgagcacagt tgcgcggcgc gcaaagtccg cgtcagccat
     1321 ggaggcattg ctatgagcga cggctacagc gacggctaca accggcagcc gactgtccgc
     1381 aaaaagcggc gcgtgaccgc cgccgaaggc gctcgaatca ccggactatc cgaacgccac
     1441 gtcgtccggc tcgtggcgca ggaacgcagc gagtggctcg ccgagcaggc tgcacgccgc
     1501 gaacgcatcc gcgcctatca cgacgacgag ggccactctt ggccgcaaac ggccaaacat
     1561 ttcgggctgc atctggacac cgttaagcga ctcggctatc gggcgaggaa agagcgtgcg
     1621 gcagaacagg aagcggctca aaaggcccac aacgaagccg acaatccacc gctgttctaa
     1681 cgcaattggg gagcgggtgt cgcgggggtt ccgtgggggg ttccgttgca acgggtcgga
     1741 caggtaaaag tcctggtaga cgctagtttt ctggtttggg ccatgcctgt ctcgttgcgt
     1801 gtttcgttgc gcccgttttg aataccagcc agacgagacg gggttctacg aatcttggtc
     1861 gataccaagc catttccgct gaatatcggg gagctcaccg ccagaatcgg tggttgtggt
     1921 gatgtacgtg gcgaactccg ttgtagtgcc tgtggtggca tccgtggcgc ggccgcggta
     1981 ccagatcttt aaatctagat aaagaagtga cgcggtctca agcgtcgagc gtcgccagcg
     2041 tgtcgaggat gtcgaagtcg tagccgtcgg cgctggcgat gtagacctgc tggtcgaatt
     2101 gactgtcgcg catacacatc gggccccggg gcccgtcgaa cccgacatcg tgcgcggatg
     2161 ccatcaggtc cggtatctcg ggggagtggg cccgctggaa gatggcctcg agcgcaatca
     2221 gaccctcgta acaggattcg gccatcgcgt tgagcggtgg cgcgtcggcg ccgtagcggg
     2281 cgacgtagct gcccatcagg tccatggcac ccgcggtggc cagtgaactg aagtacgccg
     2341 cggcgacata gaggttttcg gtggagccgg cgccgctggc cagcagcatg ttctcctcca
     2401 tcagcgggct gaaccgcgcc atgcggtcgt gcccgccggc gcgcgcgaac tcgcggttga
     2461 acaacacggc gtcctggccg acgagcagca tcaacacggc ctgcgccccc gacgcgatgg
     2521 ccttgcggac aggtgcgcgg aaatcgtcgg tgccgtacgg gacgtagatc tcccgtctga
     2581 gctcgaggtc cagatctcgg cagtacgcgc gggcggccgc ggcggaacgg cgcggccaga
     2641 tgtagtcatc gccgaccagg caccaggacc ggatgccgaa gtggtcgcgc agccaggcga
     2701 gcgcgggcgc gatctggatc tgcggtgtct cgcctgtgca gaacacgccc ggtgtgcgtt
     2761 caccgccctc gtacaacgag gtgtagacgt acgggatgcg gtcgcggacc accggggaga
     2821 tgcggttgcg cacggccgag atgtgccagc cggtcacggc gtcgagaccg tgacctcgca
     2881 accggtcggc gacggtccgg gcgacgtcgt cgccgggcgc tccgccgtcg agcacctcga
     2941 tggtgacctt gcggccctgc aggccgcctc ggtcgttgac ctccttggcc gcgagctcgg
     3001 ccacggcctc gcacgaaggc gcgaagattc ccgctggccc ttgaagcgga atcaccagcc
     3061 cgacgcggaa ctcaacctcg ccgtcctgca ctccagatca ccgtcgatcc cgtgtagtct
     3121 gcgcttcaaa gctttctagc agaaataatt cattctgaac agaccccgcc gtcgacacga
     3181 ggagacaccc accatggccg ccggacagca gcgccgcccc aacctcctgc tgccgttggt
     3241 gcgtctgacc cacctcgcgg agtcggcgat cgaacgcgtg ctcgcggact cgtcgctcaa
     3301 gatcgaggac tggcgcgtgc tcgacgagtt ggccggacgg cgcaccgtgc ccatgagcga
     3361 tctcgcgcag gccacgctga tcacgggtcc gactctcacc agaaccgtcg atcgccttgt
     3421 gtcgcaaggg atcatctacc ggactgccga tctgcatgac cgccggcggg tgctcgtggc
     3481 gttgaccccg cgggggcgga cgctgcgcaa ccgcctggtg gacgcggtag ccgaggccga
     3541 gtgtgcggct tttgaatcgt gcgggctgga cgtcgaccag ttgcgcgaac tcgtcgacac
     3601 cacctcgaat ttgacttcgt aaccacccgc gcccggcgcg ggcgttcacc cttgactttt
     3661 attttcatct ggatatattt cgggtgaatg gaaaggggtg accatgccga cctacacatt
     3721 ccgttgttcc cactgcggtc ccttcgatct cacctgcgcg atctccgagc gcgatgcggc
     3781 ggcgacctgt ccggagtgcc ggacgccggc gcgccgggtc ttcggttcgg tagggctgac
     3841 gacattcacc gcgggacatc accgcgcatt cgacgcggcg tccgcgagcg ccgaaagtcc
     3901 cacggtggtg aagtcgattc ccgcaggcgc ggaccgcccg cgggccccgc gccgcaatcc
     3961 cggtctaccg agtctgccga ggtactagcg acatgggtgg cgtcgggctc ttctacgtgg
     4021 gtgcggtgct catcatcgac gggctgatgc tgctgggccg catcagccca cgaggcgcaa
     4081 caccgctgaa cttcttcgtc ggcggactgc aggtggtgac gcctacggtg ctgatcctgc
     4141 agtccggcgg agacgcggcc gtgatcttcg cggcctccgg gctctacctg ttcggcttca
     4201 cctacctgtg ggtggccatc aacaacgtga ccgactggga cggagaaggt ctcggatggt
     4261 tctcgctgtt cgtcgcgatc gccgcactcg gctactcgtg gcacgcgttc accgccgagg
     4321 ccgacccggc gttcggggtg atctggctgc tgtgggcagt gctgtggttc atgctgttcc
     4381 tgctgctcgg cctggggcac gacgcactgg ggcccgccgt cgggttcgtc gcggtggccg
     4441 aaggcgtgat caccgccgcc gtgccggcct tcctgatcgt gtcgggcaac tgggaaaccg
     4501 gcccgctccc cgccgcggtc atcgccgtga tcggttttgc cgcagttgtt ctcgcatacc
     4561 ccatcgggcg ccgtctcgca gcgccgtcag tcaccaaccc tccaccggcc gcgctcgcgg
     4621 ccaccacccg ataagagaaa gggagtccac atctgtaaga attcttaatt aagctagcat
     4681 ttaaatggat ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca
     4741 tcatgaacaa taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat
     4801 attcaacggg aaacgtcttg ctcgaggccg cgattaaatt ccaacatgga tgctgattta
     4861 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
     4921 tatgggaagc cccatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     4981 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     5041 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccggg
     5101 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     5161 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     5221 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     5281 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     5341 aatcttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     5401 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     5461 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     5521 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     5581 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     5641 agcattacgc tgacttgaca tcgattcccg gtatcaacag ggacaccagg atttatttat
     5701 tctgcgaagt gatcttccgt cacaggtatt tattcgcgat aagctcatgg agcggcgtaa
     5761 ccgtcgcaca ggaaggacag agaaagcgcg gatctgggaa gtgacggaca gaacggtcag
     5821 gacctggatt ggggaggcgg ttgccgccgc tgctgctgac ggtgtgacgt tctctgttcc
     5881 ggtcacacca catacgttcc gccattccta tgcgatgcac atgctgtatg ccggtatacc
     5941 gctgaaagtt ctgcaaagcc tgatgggaca taagtccatc agttcaacgg aagtctacac
     6001 gaaggttttt gcgctggatg tggctgcccg gcaccgggtg cagtttgcga tgccggagtc
     6061 tgatgcggtt gcgatgctga aacaattatc ctgagaataa atgccttggc ctttatatgg
     6121 aaatgtggaa ctgagtggat atgctgtttt tgtctgttaa acagagaagc tggctgttat
     6181 ccactgagaa gcgaacgaaa cagtcgggaa aatctcccat tatcgtagag atccgcatta
     6241 ttaatctcag gagcctgtgt agcgtttata ggaagtagtg ttctgtcatg atgcctgcaa
     6301 gcggtaacga aaacgatttg aatatgcctt caggaacaat agaaatcttc gtgcggtgtt
     6361 acgttgaagt ggagcggatt atgtcagcaa tggacagaac aacctaatga acacagaacc
     6421 atgatgtggt ctgtcctttt acagccagta gtgctcgccg cagtcgagcg acagggcgaa
     6481 gccctcggct ggttgccctc gccgctgggc tggcggccgt ctatggccct gcaaacgcgc
     6541 cagaaacgcc gtcgaagccg tgtgcgagac accgcggccg gccgccggcg ttgtggatac
     6601 ctcgcggaaa acttggccct cactgacaga tgaggggcgg acgttgacac ttgaggggcc
     6661 gactcacccg gcgcggcgtt gacagatgag gggcaggctc gatttcggcc ggcgacgtgg
     6721 agctggccag cctcgcaaat cggcgaaaac gcctgatttt acgcgagttt cccacagatg
     6781 atgtggacaa gcctggggat aagtgccctg cggtattgac acttgagggg cgcgactact
     6841 gacagatgag gggcgcgatc cttgacactt gaggggcaga gtgctgacag atgaggggcg
     6901 cacctattga catttgaggg gctgtccaca ggcagaaaat ccagcatttg caagggtttc
     6961 cgcccgtttt tcggccaccg ctaacctgtc ttttaacctg cttttaaacc aatatttata
     7021 aaccttgttt ttaaccaggg ctgcgccctg tgcgcgtgac cgcgcacgcc gaaggggggt
     7081 gccccccctt ctcgaaccct cccggtcgag tgagcgagga agcaccaggg aacagcactt
     7141 atatattctg cttacacacg atgcctgaaa aaacttccct tggggttatc cacttatcca
     7201 cggggatatt tttataatta ttttttttat agtttttaga tcttcttttt tagagcgcct
     7261 tgtaggcctt tatccatgct ggttctagag aaggtgttgt gacaaattgc cctttcagtg
     7321 tgacaaatca ccctcaaatg acagtcctgt ctgtgacaaa ttgcccttaa ccctgtgaca
     7381 aattgccctc agaagaagct gttttttcac aaagttatcc ctgcttattg actctttttt
     7441 atttagtgtg acaatctaaa aacttgtcac acttcacatg gatctgtcat ggcggaaaca
     7501 gcggttatca atcacaagaa acgtaaaaat agcccgcgaa tcgtccagtc aaacgacctc
     7561 actgaggcgg catatagtct ctcccgggat caaaaacgta tgctgtatct gttcgttgac
     7621 cagatcagaa aatctgatgg caccctacag gaacatgacg gtatctgcga gatccatgtt
     7681 gctaaatatg ctgaaatatt cggattgacc tctgcggaag ccagtaagga tatacggcag
     7741 gcattgaaga gtttcgcggg gaaggaagtg gttttttatc gccctgaaga ggatgccggc
     7801 gatgaaaaag gctatgaatc ttttccttgg tttatcaaac gtgcgcacag tccatccaga
     7861 gggctttaca gtgtacatat caacccatat ctcattccct tctttatcgg gttacagaac
     7921 cggtttacgc agtttcggct tagtgaaaca aaagaaatca ccaatccgta tgccatgcgt
     7981 ttatacgaat ccctgtgtca gtatcgtaag ccggatggct caggcatcgt ctctctgaaa
     8041 atcgactgga tcatagagcg ttaccagctg cctcaaagtt accagcgtat gcctgacttc
     8101 cgccgccgct tcctgcaggt ctgtgttaat gagatcaaca gcagaactcc aatgcgcctc
     8161 tcatacattg agaaaaagaa aggccgccag acgactcata tcgtattttc cttccgcgat
     8221 atcacttcca tgacgacagg atagtctgag ggttatctgt cacagatttg agggtggttc
     8281 gtcacatttg ttctgaccta ctgagggtaa tttgtcacag ttttgctgtt tccttcagcc
     8341 tgcatggatt ttctcatact ttttgaactg taatttttaa ggaagccaaa tttgagggca
     8401 gtttgtcaca gttgatttcc ttctctttcc cttcgtcatg tgacttgata tcgggggtta
     8461 gttcgtcatc attgatgagg gttgattatc acagtttatt actctgaatt ggctatccgc
     8521 gtgtgtacct ctacctggag tttttcccac ggtggatatt tcttcttgcg ctgagcgtaa
     8581 gagctatctg acagaacagt tcttctttgc ttcctcgcca gttcgctcgc tatgctcggt
     8641 tacacggctg cggcgagcgc tagtgataat aagtgactga ggtatgtgct cttcttatct
     8701 ccttttgtag tgttgctctt attttaaaca actttgcggt tttttgatga ctttgcgatt
     8761 ttgttgttgc tttgcagtaa attgcaagat ttaataaaaa aacgcaaagc aatgattaaa
     8821 ggatgttcag aatgaaactc atggaaacac ttaaccagtg cataaacgct ggtcatgaaa
     8881 tgacgaaggc tatcgccatt gcacagttta atgatgacag cccggaagcg aggaaaataa
     8941 cccggcgctg gagaataggt gaagcagcgg atttagttgg ggtttcttct caggctatca
     9001 gagatgccga gaaagcaggg cgactaccgc acccggatat ggaaattcga ggacgggttg
     9061 agcaacgtgt tggttataca attgaacaaa ttaatcatat gcgtgatgtg tttggtacgc
     9121 gattgcgacg tgctgaagac gtatttccac cggtgatcgg ggttgctgcc cataaaggtg
     9181 gcgtttacaa aacctcagtt tctgttcatc ttgctcagga tctggctctg aaggggctac
     9241 gtgttttgct cgtggaaggt aacgaccccc agggaacagc ctcaatgtat cacggatggg
     9301 taccagatct tcatattcat gcagaagaca ctctcctgcc tttctatctt ggggaaaagg
     9361 acgatgtcac ttatgcaata aagcccactt gctggccggg gcttgacatt attccttcct
     9421 gtctggctct gcaccgtatt gaaactgagt taatgggcaa atttgatgaa ggtaaactgc
     9481 ccaccgatcc acacctgatg ctccgactgg ccattgaaac tgttgctcat gactatgatg
     9541 tcatagttat tgacagcgcg cctaacctgg gtatcggcac gattaatgtc gtatgtgctg
     9601 ctgatgtgct gattgttccc acgcctgctg agttgtttga ctacacctcc gcactgcagt
     9661 ttttcgatat gcttcgtgat ctgctcaaga acgttgatct taaagggttc gagcctgatg
     9721 tacgtatttt gcttaccaaa tacagcaata gtaatggctc tcagtccccg tggatggagg
     9781 agcaaattcg ggatgcctgg ggaagcatgg ttctaaaaaa tgttgtacgt gaaacggatg
     9841 aagttggtaa aggtcagatc cggatgagaa ctgtttttga acaggccatt gatcaacgct
     9901 cttcaactgg tgcctggaga aatgctcttt ctatttggga acctgtctgc aatgaaattt
     9961 tcgatcgtct gattaaacca cgctgggaga ttagataatg aagcgtgcgc ctgttattcc
    10021 aaaacatacg ctcaatactc aaccggttga agatacttcg ttatcgacac cagctgcccc
    10081 gatggtggat tcgttaattg cgcgcgtagg agtaatggct cgcggtaatg ccattacttt
    10141 gcctgtatgt ggtcgggatg tgaagtttac tcttgaagtg ctccggggtg atagtgttga
    10201 gaagacctct cgggtatggt caggtaatga acgtgaccag gagctgctta ctgaggacgc
    10261 actggatgat ctcatccctt cttttctact gactggtcaa cagacaccgg cgttcggtcg
    10321 aagagtatct ggtgtcatag aaattgccga tgggagtcgc cgtcgtaaag ctgctgcact
    10381 taccgaaagt gattatcgtg ttctggttgg cgagctggat gatgagcaga tggctgcatt
    10441 atccagattg ggtaacgatt atcgcccaac aagtgcttat gaacgtggtc agcgttatgc
    10501 aagccgattg cagaatgaat ttgctggaaa tatttctgcg ctggctgatg cggaaaatat
    10561 ttcacgtaag attattaccc gctgtatcaa caccgccaaa ttgcctaaat cagttgttgc
    10621 tcttttttct caccccggtg aactatctgc ccggtcaggt gatgcacttc aaaaagcctt
    10681 tacagataaa gaggaattac ttaagcagca ggcatctaac cttcatgagc agaaaaaagc
    10741 tggggtgata tttgaagctg aagaagttat cactctttta acttctgtgc ttaaaacgtc
    10801 atctgcatca agaactagtt taagctcacg acatcagttt gctcctggag cgacagtatt
    10861 gtataagggc gataaaatgg tgcttaacct ggacaggtct cgtgttccaa ctgagtgtat
    10921 agagaaaatt gaggccattc ttaaggaact tgaaaagcca gcaccctgat gcgaccacgt
    10981 tttagtctac gtttatctgt ctttacttaa tgtcctttgt tacaggccag aaagcataac
    11041 tggcctgaat attctctctg ggcccactgt tccacttgta tcgtcggtct gataatcaga
    11101 ctgggaccac ggtcccactc gtatcgtcgg tctgattatt agtctgggac cacggtccca
    11161 ctcgtatcgt cggtctgatt attagtctgg gaccacggtc ccactcgtat cgtcggtctg
    11221 ataatcagac tgggaccacg gtcccactcg tatcgtcggt ctgattatta gtctgggacc
    11281 atggtcccac tcgtatcgtc ggtctgatta ttagtctggg accacggtcc cactcgtatc
    11341 gtcggtctga ttattagtct ggaaccacgg tcccactcgt atcgtcggtc tgattattag
    11401 tctgggacca cggtcccact cgtatcgtcg gtctgattat tagtctggga ccacgatccc
    11461 actcgtgttg tcggtctgat tatcggtctg ggaccacggt cccacttgta ttgtcgatca
    11521 gactatcagc gtgagactac gattccatca atgcctgtca agggcaagta ttgacatgtc
    11581 gtcgtaacct gtagaacgga gtaacctcgg tgtgcggttg tatgcctgct gtggattgct
    11641 gctgtgtcct gcttatccac aacattttgc gcacggttat gtggacaaaa tacctggtta
    11701 cccaggccgt gccggcacgt taaccgggct gcatccgatg caagtgtgtc gctgtcgacg
    11761 agctcgcgag ctcggacatg aggttgcccc gtattcagtg tcgctgattt gtattgtctg
    11821 aagttgcttt tacgttaagt tgatgcagat caattaatac gatacctgcg tcataattga
    11881 ttatttgacg tggtttgatg gcctccacgc acgttgtgat atgtagatga taatcattat
    11941 cactttacgg gtcctttccg gtgatccgac aggttacggg gcggcgacct cgcgggtttt
    12001 cgctatttat gaaaattttc cggtttaagg cgtttccgtt cttcttcgtc ataacttaat
    12061 gtttttattt aaaataccct ctgaaaagaa aggaaacgac aggtgctgaa agcgagcttt
    12121 ttggcctctg tcgtttcctt tctctgtttt tgtccgtgga atgaacaatg gaagtccgag
    12181 ctcatcgcta ataacttcgt atagcataca ttatacgaag ttatattcga tgcggccgca
    12241 aggggttcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
    12301 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga
    12361 gaaaataccg catcaggcgc cattcgccat tcagctgcgc aactgttggg aagggcgatc
    12421 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    12481 aagttgggta acgccagggt tttcccagtc acgacgta
//
