LOCUS       sequence-175427-        9918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for reporter assay of one zebrafish super-enhancer region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9918)
  AUTHORS   Perez-Rico YA, Boeva V, Mallory AC, Bitetti A, Majello S, Barillot 
            E, Shkumatava A
  TITLE     Comparative analyses of super-enhancers reveal conserved elements in
            vertebrate genomes.
  JOURNAL   Genome Res. 2017 Feb;27(2):259-268. doi: 10.1101/gr.203679.115. Epub
            2016 Dec 13.
  PUBMED    27965291
REFERENCE   2  (bases 1 to 9918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.203679.115"; journalName: "Genome Res"; date: "2017-02";
            volume: "27"; issue: "2"; pages: "259-268"
COMMENT     Sequence Label: E1b-GFP-Tol2-Gateway dre SE-zic2a N
FEATURES             Location/Qualifiers
     source          1..9918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..125
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(150..155,156..173,174..180)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             234..893
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(300..319)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1235..1540
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1419..1438
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1581..1705)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          2409..2981
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      3055..3064
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3061..3063,3064..3066,3067..3780)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3061..3063,3064..3066,3067..3777)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3106..3127)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3367..3386)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3714..3735
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3825..3959
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3849..3868)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3903..3922
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(4396..4583,4584..4595)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(4629..4645)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4629..4645)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4682..4702)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4682..4700)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4721..4737)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4721..4737)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4734..4756)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4745..4761
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4769..4775,4776..4793,4794..4799))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4814..4835
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4952..4969)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5123..5711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5203..5222)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5882..6673,6674..6742))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6505..6524
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6743..6847)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6873..7328)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7010..7031)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7222..7241
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7455..7477
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7469..7486
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7470..7486
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7496..7515
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7496..7514
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(9366..9377,9378..9540)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
       61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      121 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      181 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
      241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
      781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
      901 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      961 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1021 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1081 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1141 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1201 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     1261 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1321 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1381 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1441 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1501 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1561 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1681 gttcagcttt cttgtacaaa gtggtgatat caagatctag accatcacac aggttgtccc
     1741 tagttaggca aaaacaaaaa agtcatcctg gtctcacagt ggtgattgaa ttctgaagta
     1801 ctatctgtgc atgacaaagg tgcagcaggt agggaataat gagttgattt gagattcagt
     1861 cctggttctc ctctctgctc aggcccccag gcttcactct ctctctccct catctcagcg
     1921 agctgtgctg taggacaggc ttatcttgag agggacctct gtgtagccaa aggctcctgg
     1981 cctgccctga ttaaaggagc gctgcttttt acccatgctg aggctagatc aatgcggaga
     2041 tgctttcagc ctgcctatat gaatttcaat atttccatat cttagttctc gccttgttgc
     2101 actgttagat cattattagg ggagctggcc ctactcaagc tgtgtgccat taagcaaatg
     2161 aagctggcct ggtgaggatg agtggcgtct tgttctcttt tctttttttt aacagggcac
     2221 taaagagccc tgtcatcttt atcttttatc tcagagctca ccggtgtttc tttcttccga
     2281 tgacctgcag atgtggcatc cctctctgag atctctcgac tctagagggt atataatgga
     2341 tcccatcgcg tctcagcctc actttgagct cctccacacg aatttcgacc gatcctgaga
     2401 acttcagggt gagtttgggg acccttgatt gttctttctt tttcgctatt gtaaaattca
     2461 tgttatatgg agggggcaaa gttttcaggg tgttgtttag aatgggaaga tgtcccttgt
     2521 atcaccatgg accctcatga taattttgtt tctttcactt tctactctgt tgacaaccat
     2581 tgtctcctct tattttcttt tcattttctg taactttttc gttaaacttt agcttgcatt
     2641 tgtaacgaat ttttaaattc acttttgttt atttgtcaga ttgtaagtac tttctctaat
     2701 cacttttttt tcaaggcaat cagggtatat tatattgtac ttcagcacag ttttagagaa
     2761 caattgttat aattaaatga taaggtagaa tatttctgca tataaattct ggctggcgtg
     2821 gaaatattct tattggtaga aacaactaca ccctggtcat catcctgcct ttctctttat
     2881 ggttacaatg atatacactg tttgagatga ggataaaata ctctgagtcc aaaccgggcc
     2941 cctctgctaa ccatgttcat gccttcttct ctttcctaca gctcctgggc aacgtgctgg
     3001 ttgttgtgct gtctcatcat tttggcaaag aattcctcga cggatccacc ggtcgccacc
     3061 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     3121 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     3181 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     3241 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     3301 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     3361 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     3421 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     3481 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     3541 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     3601 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     3661 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     3721 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     3781 agcggccgcc accgcggtgg agctcgaatt aattcatcga tgatgatcca gacatgataa
     3841 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     3901 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     3961 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     4021 aaagcaagta aaacctctac aaatgtggta tggctgatta tgatcctcta gatcagatcc
     4081 gaagatacgg ccacgggtgc tcttgatcct gtggctgatt ttggactgtg ctgctcgcag
     4141 ctgctgatga atcacatact tcctccattt tcttccactg attgactgtt ataatttccc
     4201 taatttccag gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac gtcacttcca
     4261 aaggaccaat gaacatgtct gaccaatttc atataatgtg aaaacgattt tcataggcag
     4321 aataaataac atttaaatta aactgggcat cagcgcaatt caattggttt ggtaatagca
     4381 agggaaaata gaatgaagtg atctccaaaa aataagtact ttttgactgt aaataaaatt
     4441 gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat
     4501 tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa aataatactt aagtacagta
     4561 atcaagtaaa attactcaag tactttacac ctctggttct tgacccccta ccttcagcaa
     4621 gcccagcaga tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt
     4681 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
     4741 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     4801 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     4861 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     4921 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4981 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     5041 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     5101 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     5161 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     5221 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     5281 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     5341 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     5401 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     5461 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     5521 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     5581 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5641 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5701 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5761 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5821 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5881 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5941 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     6001 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     6061 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     6121 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     6181 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     6241 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     6301 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     6361 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     6421 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     6481 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     6541 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6601 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6661 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6721 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6781 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6841 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     6901 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     6961 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     7021 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     7081 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     7141 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     7201 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     7261 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     7321 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     7381 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     7441 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata
     7501 cgactcacta tagggcgaat tgggtaccaa atagtaggaa ttacccacct gtacaagtgc
     7561 tgaaaacttg gatgaataag cccgtttgcc attttctact gctattttaa atcttttctg
     7621 tatttgtctg catttgtctt tacccttgaa taaatgtttt agttgttttt tttcaattat
     7681 gactgtgttt aacagacatt attacaataa tatgtaatag tagtacacac tattattatg
     7741 taatagtact tgttgactgt atttgagaac tggaccgagt gagtgttacg tcacccattc
     7801 aaaatgactt acttctggct ccaacaaaat gaagttaatt cagttgccat ttttcactgt
     7861 atggacatcg ccgtgttgga gctagacgtt gccaataagc aagaaagagc cgagatgcgt
     7921 cgagtctgag tcaccgttcc tatggcaacc cctctaacca atcagaagta agcttgttgg
     7981 aagtccacag cctaccactt gaaagcgggc tgcacaaaat ctgtcaaacg ttttgaacgt
     8041 tggatgtgag agcacatact tttattaagg catctggttg gtcagtttat aatatcaaca
     8101 acttgggcta cagaaaagaa aagttattac agaaattatg attaacaagt acatgttaaa
     8161 taaagatttt aatatgaatg ccaccactgg agcattcatg ccatttggag cttcttcctg
     8221 tttggatcac tagaaggagg aggtcactca ttacagttct catatacagt cgttggttgg
     8281 ttggttggtt ggtagattga ttgattgatt gattgattga ttgattgatt gattgattga
     8341 ttgattgatt gattgattga ttgattgatt ggtagtcaaa ataagaaata atttccacag
     8401 attcattaca gaaatgatta aatgcataca taaaaaactn gggggggggg gggatacaac
     8461 aacacactta agtcacattt gcctacgtaa agaaaagtaa agaaaatcaa tagctatatt
     8521 ttacatctcc tttttttgct gtcttttaat tagccttgtt ttgctgttat cttgattgaa
     8581 actgtaactt cttcacctgc ttcttttctt tgtaggtttt gccagcccag aaacaggcat
     8641 ggctgtgcaa ggccagagca ccatgcacaa tgcgctgcat gtcttcatga acggctcaat
     8701 gtcctcagtc cagggctcag ccaacgaccc catcttcctc cttcaccatg ctttcattga
     8761 caggtaacaa acacgtcatg acattagact gcacagtttt tgacaaagtt catacaatct
     8821 gttgtttata gctgctacaa ttagtgaagt ttgtgaatgt acttggatga gcagcgaaag
     8881 atcaattgag atcaattgtt agagtttggt tgccctgcag agcaaagaac aaaaaataat
     8941 ctggtggctt tactgcgtga ggttattatt ggtggaatag aaacacaaaa cataattgca
     9001 tttatttgtt taatttttta tcttatctta actttcatct tgcatatttg tttcttacat
     9061 catttctagc atctttgagc gctggctaag aactcatcag cctccccggt ccatctaccc
     9121 acgtaccaat gcaccaattg gccacaatga cggctactac atggtgccat tccttcctct
     9181 ttataggaat ggagactacc tcctgtccaa caatgctctt ggatacgagt acgcctacct
     9241 gttggaccca ggtcattgca caacaccaga aatgccctct gatctgcaaa agacgtgaat
     9301 atctgttcag acacccatat ccactctgtt ccacacaggt cagaggtttg tccaggagtt
     9361 cttgacagag gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac aagtacttag
     9421 ggaaaatttt actcaattaa aagtaaaagt atctggctag aatcttactt gagtaaaagt
     9481 aaaaaagtac tccattaaaa ttgtacttga gtattaagga agtaaaagta aaagcaagaa
     9541 agaaaactag agattcttgt ttaagctttt aatctcaaaa aacattaaat gaaatgcata
     9601 caaggtttta tcctgcttta gaactgtttg tatttaatta tcaaactata agacagacaa
     9661 tctaatgcca gtacacgcta ctcaaagttg taaaacctca gatttaactt cagtagaagc
     9721 tgattctcaa aattgttagt gtcaagccta gctcttttgg ggctgaaaag caatcctgca
     9781 gtgctgaaaa gcctctcaca ggcagccgat gcgggaagag gtgtattagt cttgatagag
     9841 aggctgcaaa tagcaggaaa cgtgagcaga gactccctgg tgtctgaaac acaggccaga
     9901 tgggccctcg aggatatc
//
