LOCUS       sequence-175428-       10451 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for reporter assay of one zebrafish super-enhancer region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10451)
  AUTHORS   Perez-Rico YA, Boeva V, Mallory AC, Bitetti A, Majello S, Barillot 
            E, Shkumatava A
  TITLE     Comparative analyses of super-enhancers reveal conserved elements in
            vertebrate genomes.
  JOURNAL   Genome Res. 2017 Feb;27(2):259-268. doi: 10.1101/gr.203679.115. Epub
            2016 Dec 13.
  PUBMED    27965291
REFERENCE   2  (bases 1 to 10451)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.203679.115"; journalName: "Genome Res"; date: "2017-02";
            volume: "27"; issue: "2"; pages: "259-268"
COMMENT     Sequence Label: E1b-GFP-Tol2-Gateway dre SE-irf2bpl C
FEATURES             Location/Qualifiers
     source          1..10451
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..125
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(150..155,156..173,174..180)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             234..893
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(300..319)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1235..1540
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1419..1438
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1581..1705)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          2944..3516
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      3590..3599
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3596..3598,3599..3601,3602..4315)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3596..3598,3599..3601,3602..4312)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3641..3662)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3902..3921)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4249..4270
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4360..4494
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4384..4403)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4438..4457
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(4931..5118,5119..5130)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5164..5180)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5164..5180)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5217..5237)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5217..5235)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5256..5272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5256..5272)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5269..5291)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5280..5296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5304..5310,5311..5328,5329..5334))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5349..5370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5487..5504)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5658..6246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5738..5757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6417..7208,7209..7277))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7040..7059
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7278..7382)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7408..7863)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7545..7566)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7757..7776
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7990..8012
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8004..8021
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8005..8021
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8031..8050
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8031..8049
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(9899..9910,9911..10073)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
       61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      121 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      181 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
      241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
      781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
      901 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      961 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1021 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1081 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1141 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1201 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     1261 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1321 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1381 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1441 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1501 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1561 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1681 gttcagcttt cttgtacaaa gtggtgatat caagatctca tctttgttaa gctttaaatg
     1741 gtacaactta aatttgcttg aaatgttact taaaattgtt cactcttgtg attaaacttt
     1801 gtgatgacct cacgaatgaa atgttcagaa ctgtagtggc tggtaatccc atctagacat
     1861 tgcaaatgtg actattgtag attcacacat tgcgatatca atgcaaaaac gatcaacccc
     1921 tactggtttg tgatgttaaa ataatgtgat gaaggtcaaa aaatgtgcca tttcatgcac
     1981 cgctgctatt tagatgcata aaacagagtc agacagcaca tgaaagaggc gaaagataca
     2041 aaaattgcac tccgacaggt attcagcggg ttctcagttc aacactttag atcaatcaag
     2101 tgaaaccatc cttgaacaat ccagcctgcg agcttttcaa gacagacgga gtctccatgg
     2161 tgccctatag tccatttcca tgtgcttaga aacaattccc tgcccccaaa actagccccc
     2221 cgccccacta ctcttctcca gccggaatct acaggaagcc gaaacaggct ctggccctca
     2281 aggagggact ggccttgaaa tgcaaattgg aattccatcc gaaaattgat gtcctttaca
     2341 agctaatgaa aaacatctga aacgctgcca aaatggcccc cctttttttt ttacaaccca
     2401 catgccatta gtcaagggta gactggagct acgtcctgtt ttgaggcaaa cattcttata
     2461 taaggagctg acgaaaatag acacaatgtt gatttaataa cgtattcaca aaagtgagaa
     2521 tcaaccacat gcaaacttat accgagagat gatagattat gcgctaaatt atattaacaa
     2581 taacatgcta ttctctaaag cttttttttt tttcgctcgg tgctagaaga gaaaaaaaat
     2641 aaactgaagg agagagtagg agtgaaaagc gggagaaaat caattctccc tctgcgcctt
     2701 taatatctgc agaaagaggg gggaagcaca cgcccgctgt agcccgcagc ttatttgcga
     2761 ggcaggaatg gaatgtctga gattgcatac attatgtgcc aaagaggccc tgggctatgg
     2821 aagatagtgc tgacatcacc gtcagatctc tcgactctag agggtatata atggatccca
     2881 tcgcgtctca gcctcacttt gagctcctcc acacgaattt cgaccgatcc tgagaacttc
     2941 agggtgagtt tggggaccct tgattgttct ttctttttcg ctattgtaaa attcatgtta
     3001 tatggagggg gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc cttgtatcac
     3061 catggaccct catgataatt ttgtttcttt cactttctac tctgttgaca accattgtct
     3121 cctcttattt tcttttcatt ttctgtaact ttttcgttaa actttagctt gcatttgtaa
     3181 cgaattttta aattcacttt tgtttatttg tcagattgta agtactttct ctaatcactt
     3241 ttttttcaag gcaatcaggg tatattatat tgtacttcag cacagtttta gagaacaatt
     3301 gttataatta aatgataagg tagaatattt ctgcatataa attctggctg gcgtggaaat
     3361 attcttattg gtagaaacaa ctacaccctg gtcatcatcc tgcctttctc tttatggtta
     3421 caatgatata cactgtttga gatgaggata aaatactctg agtccaaacc gggcccctct
     3481 gctaaccatg ttcatgcctt cttctctttc ctacagctcc tgggcaacgt gctggttgtt
     3541 gtgctgtctc atcattttgg caaagaattc ctcgacggat ccaccggtcg ccaccatggt
     3601 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     3661 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     3721 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     3781 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     3841 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     3901 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     3961 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     4021 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     4081 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     4141 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     4201 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     4261 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg
     4321 ccgccaccgc ggtggagctc gaattaattc atcgatgatg atccagacat gataagatac
     4381 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     4441 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     4501 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     4561 aagtaaaacc tctacaaatg tggtatggct gattatgatc ctctagatca gatccgaaga
     4621 tacggccacg ggtgctcttg atcctgtggc tgattttgga ctgtgctgct cgcagctgct
     4681 gatgaatcac atacttcctc cattttcttc cactgattga ctgttataat ttccctaatt
     4741 tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac ttccaaagga
     4801 ccaatgaaca tgtctgacca atttcatata atgtgaaaac gattttcata ggcagaataa
     4861 ataacattta aattaaactg ggcatcagcg caattcaatt ggtttggtaa tagcaaggga
     4921 aaatagaatg aagtgatctc caaaaaataa gtactttttg actgtaaata aaattgtaag
     4981 gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt
     5041 tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa
     5101 gtaaaattac tcaagtactt tacacctctg gttcttgacc ccctaccttc agcaagccca
     5161 gcagatccac tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt
     5221 tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     5281 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     5341 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     5401 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     5461 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     5521 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     5581 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     5641 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     5701 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     5761 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     5821 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     5881 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     5941 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     6001 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     6061 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     6121 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     6181 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     6241 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     6301 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     6361 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     6421 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     6481 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     6541 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     6601 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     6661 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     6721 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     6781 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     6841 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     6901 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     6961 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     7021 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     7081 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     7141 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     7201 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     7261 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     7321 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     7381 cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg
     7441 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     7501 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     7561 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     7621 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     7681 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     7741 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     7801 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     7861 cgtcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt
     7921 cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc
     7981 cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact
     8041 cactataggg cgaattgggt accaaatagt aggaattacc cacctgtaca agtgctgaaa
     8101 acttggatga ataagcccgt ttgccatttt ctactgctat tttaaatctt ttctgtattt
     8161 gtctgcattt gtctttaccc ttgaataaat gttttagttg ttttttttca attatgactg
     8221 tgtttaacag acattattac aataatatgt aatagtagta cacactatta ttatgtaata
     8281 gtacttgttg actgtatttg agaactggac cgagtgagtg ttacgtcacc cattcaaaat
     8341 gacttacttc tggctccaac aaaatgaagt taattcagtt gccatttttc actgtatgga
     8401 catcgccgtg ttggagctag acgttgccaa taagcaagaa agagccgaga tgcgtcgagt
     8461 ctgagtcacc gttcctatgg caacccctct aaccaatcag aagtaagctt gttggaagtc
     8521 cacagcctac cacttgaaag cgggctgcac aaaatctgtc aaacgttttg aacgttggat
     8581 gtgagagcac atacttttat taaggcatct ggttggtcag tttataatat caacaacttg
     8641 ggctacagaa aagaaaagtt attacagaaa ttatgattaa caagtacatg ttaaataaag
     8701 attttaatat gaatgccacc actggagcat tcatgccatt tggagcttct tcctgtttgg
     8761 atcactagaa ggaggaggtc actcattaca gttctcatat acagtcgttg gttggttggt
     8821 tggttggtag attgattgat tgattgattg attgattgat tgattgattg attgattgat
     8881 tgattgattg attgattgat tgattggtag tcaaaataag aaataatttc cacagattca
     8941 ttacagaaat gattaaatgc atacataaaa aactgggggg ggggggatac aacaacacac
     9001 ttaagtcaca tttgcctacg taaagaaaag taaagaaaat caatagctat attttacatc
     9061 tccttttttt gctgtctttt aattagcctt gttttgctgt tatcttgatt gaaactgtaa
     9121 cttcttcacc tgcttctttt ctttgtaggt tttgccagcc cagaaacagg catggctgtg
     9181 caaggccaga gcaccatgca caatgcgctg catgtcttca tgaacggctc aatgtcctca
     9241 gtccagggct cagccaacga ccccatcttc ctccttcacc atgctttcat tgacaggtaa
     9301 caaacacgtc atgacattag actgcacagt ttttgacaaa gttcatacaa tctgttgttt
     9361 atagctgcta caattagtga agtttgtgaa tgtacttgga tgagcagcga aagatcaatt
     9421 gagatcaatt gttagagttt ggttgccctg cagagcaaag aacaaaaaat aatctggtgg
     9481 ctttactgcg tgaggttatt attggtggaa tagaaacaca aaacataatt gcatttattt
     9541 gtttaatttt ttatcttatc ttaactttca tcttgcatat ttgtttctta catcatttct
     9601 agcatctttg agcgctggct aagaactcat cagcctcccc ggtccatcta cccacgtacc
     9661 aatgcaccaa ttggccacaa tgacggctac tacatggtgc cattccttcc tctttatagg
     9721 aatggagact acctcctgtc caacaatgct cttggatacg agtacgccta cctgttggac
     9781 ccaggtcatt gcacaacacc agaaatgccc tctgatctgc aaaagacgtg aatatctgtt
     9841 cagacaccca tatccactct gttccacaca ggtcagaggt ttgtccagga gttcttgaca
     9901 gaggtgtaaa aagtactcaa aaattttact caagtgaaag tacaagtact tagggaaaat
     9961 tttactcaat taaaagtaaa agtatctggc tagaatctta cttgagtaaa agtaaaaaag
    10021 tactccatta aaattgtact tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac
    10081 tagagattct tgtttaagct tttaatctca aaaaacatta aatgaaatgc atacaaggtt
    10141 ttatcctgct ttagaactgt ttgtatttaa ttatcaaact ataagacaga caatctaatg
    10201 ccagtacacg ctactcaaag ttgtaaaacc tcagatttaa cttcagtaga agctgattct
    10261 caaaattgtt agtgtcaagc ctagctcttt tggggctgaa aagcaatcct gcagtgctga
    10321 aaagcctctc acaggcagcc gatgcgggaa gaggtgtatt agtcttgata gagaggctgc
    10381 aaatagcagg aaacgtgagc agagactccc tggtgtctga aacacaggcc agatgggccc
    10441 tcgaggatat c
//
