LOCUS       sequence-175430-       10383 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for reporter assay of one mouse super-enhancer region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10383)
  AUTHORS   Perez-Rico YA, Boeva V, Mallory AC, Bitetti A, Majello S, Barillot 
            E, Shkumatava A
  TITLE     Comparative analyses of super-enhancers reveal conserved elements in
            vertebrate genomes.
  JOURNAL   Genome Res. 2017 Feb;27(2):259-268. doi: 10.1101/gr.203679.115. Epub
            2016 Dec 13.
  PUBMED    27965291
REFERENCE   2  (bases 1 to 10383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.203679.115"; journalName: "Genome Res"; date: "2017-02";
            volume: "27"; issue: "2"; pages: "259-268"
COMMENT     Sequence Label: E1b-GFP-Tol2-Gateway mmu SE-Zic2 U
FEATURES             Location/Qualifiers
     source          1..10383
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..125
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(150..155,156..173,174..180)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             234..893
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(300..319)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1235..1540
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1419..1438
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1581..1705)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          2875..3447
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      3521..3530
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3527..3529,3530..3532,3533..4246)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3527..3529,3530..3532,3533..4243)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3572..3593)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3833..3852)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4180..4201
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4291..4425
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4315..4334)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4369..4388
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(4862..5049,5050..5061)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5095..5111)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5095..5111)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5148..5168)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5148..5166)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5187..5203)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5187..5203)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5200..5222)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5211..5227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5235..5241,5242..5259,5260..5265))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5280..5301
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5418..5435)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5589..6177)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5669..5688)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6348..7139,7140..7208))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6971..6990
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7209..7313)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7339..7794)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7476..7497)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7688..7707
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7921..7943
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7935..7952
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7936..7952
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7962..7981
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7962..7980
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(9831..9842,9843..10005)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
       61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      121 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      181 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
      241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
      781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
      901 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      961 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1021 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1081 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1141 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1201 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     1261 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1321 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1381 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1441 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1501 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1561 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1681 gttcagcttt cttgtacaaa gtggtgatat caagatctga aattgctgtg agacactagg
     1741 gatggtagtg atagtccacc aagatttttc tacaagctgg agccagcaag gcaaacggac
     1801 cagcaaatct tcgttttagc ggagctctgt ccaggcagga agagatgacg actgtgggtt
     1861 ccagggattt tactacatca catccaagac aggaaggtct ggatggagtc agcaaagtct
     1921 aaagtcaact cttctggaca cgctcgcccc atccttttcc gacaggggcc agattcttca
     1981 ctctggcgtc ctcgcgactg agattaatct ggcacggggg ctacgattgg gctcagagac
     2041 cttggtgctc aaaatcatcc agtggcaact tgcagacctc tgagcatttc tctgaggatt
     2101 gcctctttcc cattcctcct gaactcagaa acatcagggt ctacggactc gtgtggtgca
     2161 aggggtagct aaaaatgtac tttatgctaa ggtttatgat ccctgtcaca cattcaaggg
     2221 tatgataagg ccggggagac gaacgttcct gagaccaaaa tgctgggttg ggggtggagt
     2281 ggtgtgtgtg tgtcttgttc aaatccttta gatagtaaag aactggccca atcctatagc
     2341 cacttgacgt ttgcattgaa aagctgttgg gggggggggg agcttacttt tacagtacca
     2401 aagataagtt gtgtaaaata tgcctgtgat caaatattcc catttggttc aaaattattt
     2461 ttacaattaa agatagaggt tgtttgagaa aacctttgac cttcatttga ccattctgat
     2521 tgggaatttc ttgagaaaca catcgatata tctcaaaata tactcagacg gtccaagtaa
     2581 gagtcacaaa atgttttaca tggccaaggt acacaacaga ctaatttttt gtttttttag
     2641 gtctgaaatg aacttttctt catttctcat ccttgactct cttagaagtc agataacgat
     2701 gagagtagaa gcttcaatgc agatatgtat ttcaaaattt gaatgttgtt cccccacaaa
     2761 acagcatccc tttcagatct ctcgactcta gagggtatat aatggatccc atcgcgtctc
     2821 agcctcactt tgagctcctc cacacgaatt tcgaccgatc ctgagaactt cagggtgagt
     2881 ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt atatggaggg
     2941 ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca ccatggaccc
     3001 tcatgataat tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt
     3061 ttcttttcat tttctgtaac tttttcgtta aactttagct tgcatttgta acgaattttt
     3121 aaattcactt ttgtttattt gtcagattgt aagtactttc tctaatcact tttttttcaa
     3181 ggcaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat tgttataatt
     3241 aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa tattcttatt
     3301 ggtagaaaca actacaccct ggtcatcatc ctgcctttct ctttatggtt acaatgatat
     3361 acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat
     3421 gttcatgcct tcttctcttt cctacagctc ctgggcaacg tgctggttgt tgtgctgtct
     3481 catcattttg gcaaagaatt cctcgacgga tccaccggtc gccaccatgg tgagcaaggg
     3541 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     3601 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     3661 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     3721 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     3781 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     3841 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     3901 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     3961 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     4021 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     4081 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     4141 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     4201 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgccaccg
     4261 cggtggagct cgaattaatt catcgatgat gatccagaca tgataagata cattgatgag
     4321 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     4381 gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc
     4441 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     4501 ctctacaaat gtggtatggc tgattatgat cctctagatc agatccgaag atacggccac
     4561 gggtgctctt gatcctgtgg ctgattttgg actgtgctgc tcgcagctgc tgatgaatca
     4621 catacttcct ccattttctt ccactgattg actgttataa tttccctaat ttccaggtca
     4681 aggtgctgtg cattgtggta atagatgtga catgacgtca cttccaaagg accaatgaac
     4741 atgtctgacc aatttcatat aatgtgaaaa cgattttcat aggcagaata aataacattt
     4801 aaattaaact gggcatcagc gcaattcaat tggtttggta atagcaaggg aaaatagaat
     4861 gaagtgatct ccaaaaaata agtacttttt gactgtaaat aaaattgtaa ggagtaaaaa
     4921 gtactttttt ttctaaaaaa atgtaattaa gtaaaagtaa aagtattgat ttttaattgt
     4981 actcaagtaa agtaaaaatc cccaaaaata atacttaagt acagtaatca agtaaaatta
     5041 ctcaagtact ttacacctct ggttcttgac cccctacctt cagcaagccc agcagatcca
     5101 ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
     5161 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     5221 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     5281 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     5341 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     5401 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     5461 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     5521 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     5581 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     5641 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     5701 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     5761 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     5821 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     5881 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     5941 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6001 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     6061 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     6121 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     6181 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     6241 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     6301 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     6361 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     6421 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     6481 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     6541 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     6601 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     6661 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     6721 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     6781 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     6841 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     6901 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     6961 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7021 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7081 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     7141 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     7201 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     7261 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     7321 ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt
     7381 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     7441 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     7501 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     7561 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     7621 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     7681 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     7741 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
     7801 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac
     7861 gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt
     7921 cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg
     7981 gcgaattggg taccaaatag taggaattac ccacctgtac aagtgctgaa aacttggatg
     8041 aataagcccg tttgccattt tctactgcta ttttaaatct tttctgtatt tgtctgcatt
     8101 tgtctttacc cttgaataaa tgttttagtt gttttttttc aattatgact gtgtttaaca
     8161 gacattatta caataatatg taatagtagt acacactatt attatgtaat agtacttgtt
     8221 gactgtattt gagaactgga ccgagtgagt gttacgtcac ccattcaaaa tgacttactt
     8281 ctggctccaa caaaatgaag ttaattcagt tgccattttt cactgtatgg acatcgccgt
     8341 gttggagcta gacgttgcca ataagcaaga aagagccgag atgcgtcgag tctgagtcac
     8401 cgttcctatg gcaacccctc taaccaatca gaagtaagct tgttggaagt ccacagccta
     8461 ccacttgaaa gcgggctgca caaaatctgt caaacgtttt gaacgttgga tgtgagagca
     8521 catactttta ttaaggcatc tggttggtca gtttataata tcaacaactt gggctacaga
     8581 aaagaaaagt tattacagaa attatgatta acaagtacat gttaaataaa gattttaata
     8641 tgaatgccac cactggagca ttcatgccat ttggagcttc ttcctgtttg gatcactaga
     8701 aggaggaggt cactcattac agttctcata tacagtcgtt ggttggttgg ttggttggta
     8761 gattgattga ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt
     8821 gattgattga ttgattggta gtcaaaataa gaaataattt ccacagattc attacagaaa
     8881 tgattaaatg catacataaa aaactngggg ggggggggat acaacaacac acttaagtca
     8941 catttgccta cgtaaagaaa agtaaagaaa atcaatagct atattttaca tctccttttt
     9001 ttgctgtctt ttaattagcc ttgttttgct gttatcttga ttgaaactgt aacttcttca
     9061 cctgcttctt ttctttgtag gttttgccag cccagaaaca ggcatggctg tgcaaggcca
     9121 gagcaccatg cacaatgcgc tgcatgtctt catgaacggc tcaatgtcct cagtccaggg
     9181 ctcagccaac gaccccatct tcctccttca ccatgctttc attgacaggt aacaaacacg
     9241 tcatgacatt agactgcaca gtttttgaca aagttcatac aatctgttgt ttatagctgc
     9301 tacaattagt gaagtttgtg aatgtacttg gatgagcagc gaaagatcaa ttgagatcaa
     9361 ttgttagagt ttggttgccc tgcagagcaa agaacaaaaa ataatctggt ggctttactg
     9421 cgtgaggtta ttattggtgg aatagaaaca caaaacataa ttgcatttat ttgtttaatt
     9481 ttttatctta tcttaacttt catcttgcat atttgtttct tacatcattt ctagcatctt
     9541 tgagcgctgg ctaagaactc atcagcctcc ccggtccatc tacccacgta ccaatgcacc
     9601 aattggccac aatgacggct actacatggt gccattcctt cctctttata ggaatggaga
     9661 ctacctcctg tccaacaatg ctcttggata cgagtacgcc tacctgttgg acccaggtca
     9721 ttgcacaaca ccagaaatgc cctctgatct gcaaaagacg tgaatatctg ttcagacacc
     9781 catatccact ctgttccaca caggtcagag gtttgtccag gagttcttga cagaggtgta
     9841 aaaagtactc aaaaatttta ctcaagtgaa agtacaagta cttagggaaa attttactca
     9901 attaaaagta aaagtatctg gctagaatct tacttgagta aaagtaaaaa agtactccat
     9961 taaaattgta cttgagtatt aaggaagtaa aagtaaaagc aagaaagaaa actagagatt
    10021 cttgtttaag cttttaatct caaaaaacat taaatgaaat gcatacaagg ttttatcctg
    10081 ctttagaact gtttgtattt aattatcaaa ctataagaca gacaatctaa tgccagtaca
    10141 cgctactcaa agttgtaaaa cctcagattt aacttcagta gaagctgatt ctcaaaattg
    10201 ttagtgtcaa gcctagctct tttggggctg aaaagcaatc ctgcagtgct gaaaagcctc
    10261 tcacaggcag ccgatgcggg aagaggtgta ttagtcttga tagagaggct gcaaatagca
    10321 ggaaacgtga gcagagactc cctggtgtct gaaacacagg ccagatgggc cctcgaggat
    10381 atc
//
