LOCUS       sequence-175431-       11041 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector for reporter assay of one zebrafish super-enhancer region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11041)
  AUTHORS   Perez-Rico YA, Boeva V, Mallory AC, Bitetti A, Majello S, Barillot 
            E, Shkumatava A
  TITLE     Comparative analyses of super-enhancers reveal conserved elements in
            vertebrate genomes.
  JOURNAL   Genome Res. 2017 Feb;27(2):259-268. doi: 10.1101/gr.203679.115. Epub
            2016 Dec 13.
  PUBMED    27965291
REFERENCE   2  (bases 1 to 11041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.203679.115"; journalName: "Genome Res"; date: "2017-02";
            volume: "27"; issue: "2"; pages: "259-268"
COMMENT     Sequence Label: E1b-GFP-Tol2-Gateway dre SE-irf2bpl A
FEATURES             Location/Qualifiers
     source          1..11041
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..125
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(150..155,156..173,174..180)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             234..893
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(300..319)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1235..1540
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1419..1438
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1581..1705)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          3534..4106
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      4180..4189
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4186..4188,4189..4191,4192..4905)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4186..4188,4189..4191,4192..4902)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4231..4252)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4492..4511)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4839..4860
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4950..5084
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4974..4993)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5028..5047
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(5521..5708,5709..5720)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5754..5770)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5754..5770)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5807..5827)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5807..5825)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5846..5862)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5846..5862)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5859..5881)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5870..5886
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5894..5900,5901..5918,5919..5924))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5939..5960
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6077..6094)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6248..6836)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6328..6347)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7007..7798,7799..7867))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7630..7649
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7868..7972)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7998..8453)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8135..8156)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8347..8366
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8580..8602
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8594..8611
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8595..8611
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8621..8640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8621..8639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(10489..10500,10501..10663)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
       61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      121 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      181 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
      241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
      781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
      901 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      961 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1021 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1081 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1141 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1201 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     1261 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1321 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1381 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1441 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1501 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1561 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1681 gttcagcttt cttgtacaaa gtggtgatat caagatctga gttataccat ttcaagatcg
     1741 gtgtgaacat catcacacag ccaagcccga aataaaccat ctgcgaaaac aatttacaca
     1801 tacttttttc cccgttctca tttcaagtct ggaagtgtga agggaagtct ggtacagctg
     1861 ctgacattcc tctaacactc tgtaatgaag caccatcgcg ggtctggttc acgcaacaga
     1921 tgggctacag gtggggcgtc acactcagcc caggtgacag gaggaaggtg attacgactc
     1981 tactcatcca aaaaaaaaag ctcagtttgc tggggggttc tggtcaggct gcaacgatgt
     2041 caaaaatcgc cattaaccaa tcagcaggca ggagctgagt tcatcaagcg cagcacacag
     2101 caggagtcag caagatatgc agtgaaaatg ttgggtaaat gactctggag aaaacagagt
     2161 ggaagacaaa cagggagtac tgacacagta catgactgta agtgtttggt gatgatgaat
     2221 gttaaataca aatgttattt cagaagagaa ctgcttgttt ttgattctag acatgtcggt
     2281 agaacgcaag aagactatta ttatgggaag aagtacaaat gagaatctat ttaaggtaaa
     2341 tctcacacaa aataaactga aaaggtcaca aataattctg taatcatttt catgactcta
     2401 gaacaagtgg caacctcccg ctctacctcg tgaagtaaat gaaactgcaa ttcatcgaca
     2461 ttccgctagt cctggcacca tataaagagt aaatgtccat tgaccccact gttaaaatga
     2521 ccaactttac agcagaaaaa aaaggtgttt acagtctggt acaaagaatg atttcggttc
     2581 atacagataa tattgccctt catgacaact gtgagggggg tgtattgctt tataactcat
     2641 ccgtttagtt tatattaagt tatattaagt ctgcatagtt aagggtatgg ccacttgagt
     2701 gacagctagg tcactctagt ttcgctggtc gctgtcactt cacgtcagct gattccggct
     2761 gattagctac tgagctcagc atatacatca tatttttgtt gtgttttatg tggctttaaa
     2821 gagtcagttg cttcttggaa ttatttctta caattatgag atgatatggc acactgtgtg
     2881 cacttaattt ttctgtcatt tatttctcat ttgctgtata ttgtattatc ttgatgatgt
     2941 caatatatta catgttttat agatgctttg tcaatactgt acaaaatgta aaaccaataa
     3001 agcaacctga acttgaatga gagaaagagc gaagcagatt ttagttggtg cacatggctc
     3061 gtgaatagca taaaagagaa atatttttta tttatttatt tactttaacc tattgaaaaa
     3121 gaccttctca gaatatttta acacctcatc gccactttaa gttctaatca gtatcaaatc
     3181 tttaacttaa taaacagttg tagttaaata aaagaatgta gcataaccat gcaacagaac
     3241 tcagataagc tcggaagaaa tgaagcttga aagaattaac tacgcggttg ttttcagcga
     3301 caggtttagg cccctgtttg aactcgtctt tctccaacca ggaatatgca aacttatgtt
     3361 ttcccatgtt gtcatctgtt tcgctctatg gtttctctac gtgtggagag cataacatca
     3421 ccatcccgtg tccagatctc tcgactctag agggtatata atggatccca tcgcgtctca
     3481 gcctcacttt gagctcctcc acacgaattt cgaccgatcc tgagaacttc agggtgagtt
     3541 tggggaccct tgattgttct ttctttttcg ctattgtaaa attcatgtta tatggagggg
     3601 gcaaagtttt cagggtgttg tttagaatgg gaagatgtcc cttgtatcac catggaccct
     3661 catgataatt ttgtttcttt cactttctac tctgttgaca accattgtct cctcttattt
     3721 tcttttcatt ttctgtaact ttttcgttaa actttagctt gcatttgtaa cgaattttta
     3781 aattcacttt tgtttatttg tcagattgta agtactttct ctaatcactt ttttttcaag
     3841 gcaatcaggg tatattatat tgtacttcag cacagtttta gagaacaatt gttataatta
     3901 aatgataagg tagaatattt ctgcatataa attctggctg gcgtggaaat attcttattg
     3961 gtagaaacaa ctacaccctg gtcatcatcc tgcctttctc tttatggtta caatgatata
     4021 cactgtttga gatgaggata aaatactctg agtccaaacc gggcccctct gctaaccatg
     4081 ttcatgcctt cttctctttc ctacagctcc tgggcaacgt gctggttgtt gtgctgtctc
     4141 atcattttgg caaagaattc ctcgacggat ccaccggtcg ccaccatggt gagcaagggc
     4201 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc
     4261 cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg
     4321 aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg
     4381 acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc
     4441 aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc
     4501 aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag
     4561 ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac
     4621 tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac
     4681 ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag
     4741 aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag
     4801 tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg
     4861 accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgccaccgc
     4921 ggtggagctc gaattaattc atcgatgatg atccagacat gataagatac attgatgagt
     4981 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg
     5041 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca
     5101 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc
     5161 tctacaaatg tggtatggct gattatgatc ctctagatca gatccgaaga tacggccacg
     5221 ggtgctcttg atcctgtggc tgattttgga ctgtgctgct cgcagctgct gatgaatcac
     5281 atacttcctc cattttcttc cactgattga ctgttataat ttccctaatt tccaggtcaa
     5341 ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac ttccaaagga ccaatgaaca
     5401 tgtctgacca atttcatata atgtgaaaac gattttcata ggcagaataa ataacattta
     5461 aattaaactg ggcatcagcg caattcaatt ggtttggtaa tagcaaggga aaatagaatg
     5521 aagtgatctc caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag
     5581 tacttttttt tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta
     5641 ctcaagtaaa gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac
     5701 tcaagtactt tacacctctg gttcttgacc ccctaccttc agcaagccca gcagatccac
     5761 tagttctaga gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt
     5821 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     5881 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5941 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     6001 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     6061 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     6121 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     6181 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     6241 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     6301 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     6361 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     6421 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     6481 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     6541 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     6601 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     6661 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     6721 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     6781 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     6841 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     6901 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     6961 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     7021 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     7081 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     7141 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     7201 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     7261 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     7321 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     7381 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     7441 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     7501 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     7561 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     7621 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     7681 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     7741 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     7801 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     7861 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     7921 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     7981 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     8041 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     8101 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     8161 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     8221 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     8281 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     8341 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     8401 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     8461 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     8521 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     8581 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
     8641 cgaattgggt accaaatagt aggaattacc cacctgtaca agtgctgaaa acttggatga
     8701 ataagcccgt ttgccatttt ctactgctat tttaaatctt ttctgtattt gtctgcattt
     8761 gtctttaccc ttgaataaat gttttagttg ttttttttca attatgactg tgtttaacag
     8821 acattattac aataatatgt aatagtagta cacactatta ttatgtaata gtacttgttg
     8881 actgtatttg agaactggac cgagtgagtg ttacgtcacc cattcaaaat gacttacttc
     8941 tggctccaac aaaatgaagt taattcagtt gccatttttc actgtatgga catcgccgtg
     9001 ttggagctag acgttgccaa taagcaagaa agagccgaga tgcgtcgagt ctgagtcacc
     9061 gttcctatgg caacccctct aaccaatcag aagtaagctt gttggaagtc cacagcctac
     9121 cacttgaaag cgggctgcac aaaatctgtc aaacgttttg aacgttggat gtgagagcac
     9181 atacttttat taaggcatct ggttggtcag tttataatat caacaacttg ggctacagaa
     9241 aagaaaagtt attacagaaa ttatgattaa caagtacatg ttaaataaag attttaatat
     9301 gaatgccacc actggagcat tcatgccatt tggagcttct tcctgtttgg atcactagaa
     9361 ggaggaggtc actcattaca gttctcatat acagtcgttg gttggttggt tggttggtag
     9421 attgattgat tgattgattg attgattgat tgattgattg attgattgat tgattgattg
     9481 attgattgat tgattggtag tcaaaataag aaataatttc cacagattca ttacagaaat
     9541 gattaaatgc atacataaaa aactgggggg ggggggatac aacaacacac ttaagtcaca
     9601 tttgcctacg taaagaaaag taaagaaaat caatagctat attttacatc tccttttttt
     9661 gctgtctttt aattagcctt gttttgctgt tatcttgatt gaaactgtaa cttcttcacc
     9721 tgcttctttt ctttgtaggt tttgccagcc cagaaacagg catggctgtg caaggccaga
     9781 gcaccatgca caatgcgctg catgtcttca tgaacggctc aatgtcctca gtccagggct
     9841 cagccaacga ccccatcttc ctccttcacc atgctttcat tgacaggtaa caaacacgtc
     9901 atgacattag actgcacagt ttttgacaaa gttcatacaa tctgttgttt atagctgcta
     9961 caattagtga agtttgtgaa tgtacttgga tgagcagcga aagatcaatt gagatcaatt
    10021 gttagagttt ggttgccctg cagagcaaag aacaaaaaat aatctggtgg ctttactgcg
    10081 tgaggttatt attggtggaa tagaaacaca aaacataatt gcatttattt gtttaatttt
    10141 ttatcttatc ttaactttca tcttgcatat ttgtttctta catcatttct agcatctttg
    10201 agcgctggct aagaactcat cagcctcccc ggtccatcta cccacgtacc aatgcaccaa
    10261 ttggccacaa tgacggctac tacatggtgc cattccttcc tctttatagg aatggagact
    10321 acctcctgtc caacaatgct cttggatacg agtacgccta cctgttggac ccaggtcatt
    10381 gcacaacacc agaaatgccc tctgatctgc aaaagacgtg aatatctgtt cagacaccca
    10441 tatccactct gttccacaca ggtcagaggt ttgtccagga gttcttgaca gaggtgtaaa
    10501 aagtactcaa aaattttact caagtgaaag tacaagtact tagggaaaat tttactcaat
    10561 taaaagtaaa agtatctggc tagaatctta cttgagtaaa agtaaaaaag tactccatta
    10621 aaattgtact tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac tagagattct
    10681 tgtttaagct tttaatctca aaaaacatta aatgaaatgc atacaaggtt ttatcctgct
    10741 ttagaactgt ttgtatttaa ttatcaaact ataagacaga caatctaatg ccagtacacg
    10801 ctactcaaag ttgtaaaacc tcagatttaa cttcagtaga agctgattct caaaattgtt
    10861 agtgtcaagc ctagctcttt tggggctgaa aagcaatcct gcagtgctga aaagcctctc
    10921 acaggcagcc gatgcgggaa gaggtgtatt agtcttgata gagaggctgc aaatagcagg
    10981 aaacgtgagc agagactccc tggtgtctga aacacaggcc agatgggccc tcgaggatat
    11041 c
//
