LOCUS       sequence-175433-       10587 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector for reporter assay of one mouse super-enhancer region.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 10587)
  AUTHORS   Perez-Rico YA, Boeva V, Mallory AC, Bitetti A, Majello S, Barillot 
            E, Shkumatava A
  TITLE     Comparative analyses of super-enhancers reveal conserved elements in
            vertebrate genomes.
  JOURNAL   Genome Res. 2017 Feb;27(2):259-268. doi: 10.1101/gr.203679.115. Epub
            2016 Dec 13.
  PUBMED    27965291
REFERENCE   2  (bases 1 to 10587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.203679.115"; journalName: "Genome Res"; date: "2017-02";
            volume: "27"; issue: "2"; pages: "259-268"
COMMENT     Sequence Label: E1b-GFP-Tol2-Gateway mmu SE-Zic2 T
FEATURES             Location/Qualifiers
     source          1..10587
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    1..125
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(150..155,156..173,174..180)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             234..893
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(300..319)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1235..1540
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1419..1438
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1581..1705)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          3080..3652
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      3726..3735
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3732..3734,3735..3737,3738..4451)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3732..3734,3735..3737,3738..4448)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3777..3798)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4038..4057)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4385..4406
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4496..4630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4520..4539)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4574..4593
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(5067..5254,5255..5266)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(5300..5316)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5300..5316)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(5353..5373)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5353..5371)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5392..5408)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5392..5408)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5405..5427)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5416..5432
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5440..5446,5447..5464,5465..5470))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5485..5506
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5623..5640)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5794..6382)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5874..5893)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6553..7344,7345..7413))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7176..7195
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7414..7518)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7544..7999)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7681..7702)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7893..7912
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8126..8148
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8140..8157
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8141..8157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8167..8186
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8167..8185
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(10035..10046,10047..10209)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 acaagtttgt acaaaaaagc tgaacgagaa acgtaaaatg atataaatat caatatatta
       61 aattagattt tgcataaaaa acagactaca taatactgta aaacacaaca tatccagtca
      121 ctatggcggc cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg
      181 tgtggatttt gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga
      241 aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
      301 aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
      361 cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
      421 ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
      481 tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
      541 catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
      601 atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
      661 ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
      721 tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
      781 tgctgatgcc gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca
      841 gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taaacgcgtg
      901 gatccggctt actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta
      961 taagaatata tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca
     1021 gcgtattaca gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc
     1081 aatatctccg gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa
     1141 cgctggaaag cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac
     1201 ggctcttttg ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa
     1261 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     1321 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     1381 actttacccg gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     1441 cagtgtgccg gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     1501 catcaaaaac gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca
     1561 cagccagtct gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag
     1621 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
     1681 gttcagcttt cttgtacaaa gtggtgatat caagatctat ttgggtgggt gactagaaag
     1741 ggaatattta caaagtgagt tgaatgaata ttcaaattca tgaccaaacc cagaccctaa
     1801 aaagataatt gactcaggta aatcgacttg agatgctaat tcttaaaaga aagggctggg
     1861 gagcaaaatt tcaatcacca ccaataaggc gccaataaac cgcctgccaa ttaaacctga
     1921 gaataggcct agctacttgg tcatcaggaa ggctcactgt ggatttccgg aattgccttg
     1981 ctttataaaa gggcttttta ttttgtagac gccctttttg ttattgtgat gacctgcctg
     2041 accccaggtg cgctgtttct ttttccagct gtttgggagc cagccgaaac gcacttaaat
     2101 cccgactttc taaggacacg ataactgtct tagacacttc ctaaaaaaac aacaatttcc
     2161 agagcgttga agtgaataat gtactggttt ccaaagcagg gagtgataga tgtattcatg
     2221 caaattctgt ccttttctgt ggaactagcc tattctgatg gtggaactgg tctttaggac
     2281 cactttccca aacctgtgcc ctaatctgca ctttgtgctg tagaggccct tgtatgaagg
     2341 cagagcaaac cttggaaata tcggtgctgg aggcaacaag agctttgggg cctgtggatt
     2401 tacagacctc acgtttgctc taggaacccc aacatctttt cagctcccca aacaaagacg
     2461 cggggaggtg gaaggcacag acaatcccca gttggctaga acccgatagc cacgctgctg
     2521 cttagcttcc taaaggccac agaacttggt gctcggggat tctgaggctg gcttcccgct
     2581 gtcgggaggg agggctgtgt ctcccagttc caggtgctgt ccgtgcctca cttaacacag
     2641 tcaagtttcc tacgatgggt gtgggcaatt aataagctct aagggaagtg aattggacat
     2701 ttcctggctt ttgagccttt gtttgattgt ttctccaaga gggcgggcgg tggggggggg
     2761 agacatttgg gggacggggg tgaacaagaa aggaactggg gagacccacc gagatgcctt
     2821 ttttattgcc cttgcaattg ctttacaact cgccagtccc gcgtggtttc tgagaagcgg
     2881 cctccttgcg gaagggctaa gtgccggttg gatttgtaaa gtctaaggcc cttcaggtta
     2941 agcccgagac gggttcaagg gcgaacgggg cacacatgga gatctctcga ctctagaggg
     3001 tatataatgg atcccatcgc gtctcagcct cactttgagc tcctccacac gaatttcgac
     3061 cgatcctgag aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat
     3121 tgtaaaattc atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag
     3181 atgtcccttg tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg
     3241 ttgacaacca ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt
     3301 tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta
     3361 ctttctctaa tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca
     3421 gttttagaga acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc
     3481 tggctggcgt ggaaatattc ttattggtag aaacaactac accctggtca tcatcctgcc
     3541 tttctcttta tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc
     3601 caaaccgggc ccctctgcta accatgttca tgccttcttc tctttcctac agctcctggg
     3661 caacgtgctg gttgttgtgc tgtctcatca ttttggcaaa gaattcctcg acggatccac
     3721 cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     3781 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     3841 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     3901 cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg
     3961 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     4021 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     4081 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     4141 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
     4201 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
     4261 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     4321 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
     4381 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     4441 tgtacaagta aagcggccgc caccgcggtg gagctcgaat taattcatcg atgatgatcc
     4501 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     4561 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     4621 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     4681 ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt atgatcctct
     4741 agatcagatc cgaagatacg gccacgggtg ctcttgatcc tgtggctgat tttggactgt
     4801 gctgctcgca gctgctgatg aatcacatac ttcctccatt ttcttccact gattgactgt
     4861 tataatttcc ctaatttcca ggtcaaggtg ctgtgcattg tggtaataga tgtgacatga
     4921 cgtcacttcc aaaggaccaa tgaacatgtc tgaccaattt catataatgt gaaaacgatt
     4981 ttcataggca gaataaataa catttaaatt aaactgggca tcagcgcaat tcaattggtt
     5041 tggtaatagc aagggaaaat agaatgaagt gatctccaaa aaataagtac tttttgactg
     5101 taaataaaat tgtaaggagt aaaaagtact tttttttcta aaaaaatgta attaagtaaa
     5161 agtaaaagta ttgattttta attgtactca agtaaagtaa aaatccccaa aaataatact
     5221 taagtacagt aatcaagtaa aattactcaa gtactttaca cctctggttc ttgaccccct
     5281 accttcagca agcccagcag atccactagt tctagagcgg ccgccaccgc ggtggagctc
     5341 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     5401 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     5461 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     5521 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     5581 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     5641 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     5701 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     5761 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     5821 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     5881 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     5941 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     6001 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     6061 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     6121 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     6181 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     6241 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     6301 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     6361 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6421 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6481 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6541 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6601 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6661 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6721 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6781 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6841 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     6901 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     6961 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     7021 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     7081 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     7141 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     7201 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     7261 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     7321 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     7381 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7441 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7501 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     7561 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     7621 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     7681 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     7741 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     7801 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     7861 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     7921 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     7981 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     8041 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     8101 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag
     8161 cgcgcgtaat acgactcact atagggcgaa ttgggtacca aatagtagga attacccacc
     8221 tgtacaagtg ctgaaaactt ggatgaataa gcccgtttgc cattttctac tgctatttta
     8281 aatcttttct gtatttgtct gcatttgtct ttacccttga ataaatgttt tagttgtttt
     8341 ttttcaatta tgactgtgtt taacagacat tattacaata atatgtaata gtagtacaca
     8401 ctattattat gtaatagtac ttgttgactg tatttgagaa ctggaccgag tgagtgttac
     8461 gtcacccatt caaaatgact tacttctggc tccaacaaaa tgaagttaat tcagttgcca
     8521 tttttcactg tatggacatc gccgtgttgg agctagacgt tgccaataag caagaaagag
     8581 ccgagatgcg tcgagtctga gtcaccgttc ctatggcaac ccctctaacc aatcagaagt
     8641 aagcttgttg gaagtccaca gcctaccact tgaaagcggg ctgcacaaaa tctgtcaaac
     8701 gttttgaacg ttggatgtga gagcacatac ttttattaag gcatctggtt ggtcagttta
     8761 taatatcaac aacttgggct acagaaaaga aaagttatta cagaaattat gattaacaag
     8821 tacatgttaa ataaagattt taatatgaat gccaccactg gagcattcat gccatttgga
     8881 gcttcttcct gtttggatca ctagaaggag gaggtcactc attacagttc tcatatacag
     8941 tcgttggttg gttggttggt tggtagattg attgattgat tgattgattg attgattgat
     9001 tgattgattg attgattgat tgattgattg attgattgat tggtagtcaa aataagaaat
     9061 aatttccaca gattcattac agaaatgatt aaatgcatac ataaaaaact gggggggggg
     9121 ggatacaaca acacacttaa gtcacatttg cctacgtaaa gaaaagtaaa gaaaatcaat
     9181 agctatattt tacatctcct ttttttgctg tcttttaatt agccttgttt tgctgttatc
     9241 ttgattgaaa ctgtaacttc ttcacctgct tcttttcttt gtaggttttg ccagcccaga
     9301 aacaggcatg gctgtgcaag gccagagcac catgcacaat gcgctgcatg tcttcatgaa
     9361 cggctcaatg tcctcagtcc agggctcagc caacgacccc atcttcctcc ttcaccatgc
     9421 tttcattgac aggtaacaaa cacgtcatga cattagactg cacagttttt gacaaagttc
     9481 atacaatctg ttgtttatag ctgctacaat tagtgaagtt tgtgaatgta cttggatgag
     9541 cagcgaaaga tcaattgaga tcaattgtta gagtttggtt gccctgcaga gcaaagaaca
     9601 aaaaataatc tggtggcttt actgcgtgag gttattattg gtggaataga aacacaaaac
     9661 ataattgcat ttatttgttt aattttttat cttatcttaa ctttcatctt gcatatttgt
     9721 ttcttacatc atttctagca tctttgagcg ctggctaaga actcatcagc ctccccggtc
     9781 catctaccca cgtaccaatg caccaattgg ccacaatgac ggctactaca tggtgccatt
     9841 ccttcctctt tataggaatg gagactacct cctgtccaac aatgctcttg gatacgagta
     9901 cgcctacctg ttggacccag gtcattgcac aacaccagaa atgccctctg atctgcaaaa
     9961 gacgtgaata tctgttcaga cacccatatc cactctgttc cacacaggtc agaggtttgt
    10021 ccaggagttc ttgacagagg tgtaaaaagt actcaaaaat tttactcaag tgaaagtaca
    10081 agtacttagg gaaaatttta ctcaattaaa agtaaaagta tctggctaga atcttacttg
    10141 agtaaaagta aaaaagtact ccattaaaat tgtacttgag tattaaggaa gtaaaagtaa
    10201 aagcaagaaa gaaaactaga gattcttgtt taagctttta atctcaaaaa acattaaatg
    10261 aaatgcatac aaggttttat cctgctttag aactgtttgt atttaattat caaactataa
    10321 gacagacaat ctaatgccag tacacgctac tcaaagttgt aaaacctcag atttaacttc
    10381 agtagaagct gattctcaaa attgttagtg tcaagcctag ctcttttggg gctgaaaagc
    10441 aatcctgcag tgctgaaaag cctctcacag gcagccgatg cgggaagagg tgtattagtc
    10501 ttgatagaga ggctgcaaat agcaggaaac gtgagcagag actccctggt gtctgaaaca
    10561 caggccagat gggccctcga ggatatc
//
