LOCUS       sequence-17545-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Tee AR, Fingar DC, Manning BD, Kwiatkowski DJ, Cantley LC, Blenis J
  TITLE     Tuberous sclerosis complex-1 and -2 gene products function together 
            to inhibit mammalian target of rapamycin (mTOR)-mediated downstream 
            signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2002 Oct 15. 99(21):13571-6.
  PUBMED    12271141
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2002 Oct 15. 99(21):13571-6."
COMMENT     Sequence Label: pRK7-FLAG-TSC2
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..72
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctcccaggtc cactgcacct cggttctaag cttctgcagg tcgacatgga ctataaggac
       61 gatgatgaca aagccaaacc aacaagcaaa gattcaggct tgaaggagaa gtttaagatt
      121 ctgttgggac tgggaacacc gaggccaaat cccaggtctg cagagggtaa acagacggag
      181 tttatcatca ccgcggaaat actgagagaa ctgagcatgg aatgtggcct caacaatcgc
      241 atccggatga tagggcagat ttgtgaagtc gcaaaaacca agaaatttga agagcacgca
      301 gtggaagcac tctggaaggc ggtcgcggat ctgttgcagc cggagcggcc gctggaggcc
      361 cggcacgcgg tgctggctct gctgaaggcc atcgtgcagg ggcagggcga gcgtttgggg
      421 gtcctcagag ccctcttctt taaggtcatc aaggattacc cttccaacga agaccttcac
      481 gaaaggctgg aggttttcaa ggccctcaca gacaatggga gacacatcac ctacttggag
      541 gaagagctgg ctgactttgt cctgcagtgg atggatgttg gcttgtcctc ggaattcctt
      601 ctggtgctgg tgaacttggt caaattcaat agctgttacc tcgacgagta catcgcaagg
      661 atggttcaga tgatctgtct gctgtgcgtc cggaccgcgt cctctgtgga catagaggtc
      721 tccctgcagg tgctggacgc cgtggtctgc tacaactgcc tgccggctga gagcctcccg
      781 ctgttcatcg ttaccctctg tcgcaccatc aacgtcaagg agctctgcga gccttgctgg
      841 aagctgatgc ggaacctcct tggcacccac ctgggccaca gcgccatcta caacatgtgc
      901 cacctcat
//
