LOCUS       sequence-175473-        9094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Module A, Promoter: ZmUbi, Gene: Csy4-P2A-TaCas9, Terminator: HSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 9094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pMOD_A1510
FEATURES             Location/Qualifiers
     source          1..9094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      80..668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     569..588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     822..839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             3454..3510
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3511..7614
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             3511..7614
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             7615..7662
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      7674..7922
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        8002..8106
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gccttgacgg caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
       61 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
      121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ctaactcttt
      181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
      241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
      301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
      361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgctcacagc
      421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
      481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
      541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
      601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
      661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
      721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
      781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
      841 aagcggaaga gcgcccacct gctcatgatc tgcaggcgcg cctgcagtgc agcgtgaccc
      901 ggtcgtgccc ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac
      961 atattttttt tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa
     1021 actttactct acgaataata taatctatag tactacaata atatcagtgt tttagagaat
     1081 catataaatg aacagttaga catggtctaa aggacaattg agtattttga caacaggact
     1141 ctacagtttt atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc
     1201 tatataatac ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt
     1261 tatagactaa tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa
     1321 ctaaaactct attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa
     1381 gtgactaaaa attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc
     1441 ttgtttcgag tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa
     1501 ccagcgaacc agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg
     1561 ctgcctctgg acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca
     1621 tccagaaatt gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc
     1681 ctctcacggc accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc
     1741 ctcgcccgcc gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt
     1801 tcggagcgca cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt
     1861 caaggtacgc cgctcgtcct cccccccccc cctctctacc ttctctagat cggcgttccg
     1921 gtccatggtt agggcccggt agttctactt ctgttcatgt ttgtgttaga tccgtgtttg
     1981 tgttagatcc gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca gacacgttct
     2041 gattgctaac ttgccagtgt ttctctttgg ggaatcctgg gatggctcta gccgttccgc
     2101 agacgggatc gatttcatga ttttttttgt ttcgttgcat agggtttggt ttgccctttt
     2161 cctttatttc aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat gctttttttt
     2221 gtcttggttg tgatgatgtg gtctggttgg gcggtcgttc tagatcggag tagaattaat
     2281 tctgtttcaa actacctggt ggatttatta attttggatc tgtatgtgtg tgccatacat
     2341 attcatagtt acgaattgaa gatgatggat ggaaatatcg atctaggata ggtatacatg
     2401 ttgatgcggg ttttactgat gcatatacag agatgctttt tgttcgcttg gttgtgatga
     2461 tgtggtgtgg ttgggcggtc gttcattcgt tctagatcgg agtagaatac tgtttcaaac
     2521 tacctggtgt atttattaat tttggaactg tatgtgtgtg tcatacatct tcatagttac
     2581 gagtttaaga tggatggaaa tatcgatcta ggataggtat acatgttgat gtgggtttta
     2641 ctgatgcata tacatgatgg catatgcagc atctattcat atgctctaac cttgagtacc
     2701 tatctattat aataaacaag tatgttttat aattattttg atcttgatat acttggatga
     2761 tggcatatgc agcagctata tgtggatttt tttagccctg ccttcatacg ctatttattt
     2821 gcttggtact gtttcttttg tcgatgctca ccctgttgtt tggtgttact tcctgcagga
     2881 agcatggacc actacctcga catcaggctc aggccagacc cagagttccc accagcccag
     2941 ctcatgtccg tcctcttcgg caagctccac caggccctcg tggcccaggg cggcgacagg
     3001 atcggcgtgt ccttcccaga cctcgacgag tccaggtcca ggctcggcga gaggctccgc
     3061 atccacgcct ccgccgacga cctcagggcc ctcctcgcca ggccgtggct ggagggcctc
     3121 agggaccacc tccagttcgg cgagccagcc gtggtgccac acccaacccc atacaggcaa
     3181 gtgtccaggg tgcaagccaa gtccaaccca gagaggctca ggaggaggct catgaggagg
     3241 cacgacctct ccgaggaaga ggccaggaag cgcatcccag acaccgtggc cagggccctc
     3301 gacctcccat tcgtgaccct caggtcccag tccaccggcc agcacttccg cctcttcatc
     3361 aggcacggcc cactccaggt gaccgccgag gagggcggct ttacctgcta cggcctctcc
     3421 aagggcggct tcgtgccgtg gttcggctcc ggcgccacca acttctccct cctcaagcaa
     3481 gccggcgacg tggaggagaa cccaggccca atggacaaga agtactcgat cggcctcgac
     3541 atcgggacga actcagttgg ctgggccgtg atcaccgacg agtacaaggt gccctctaag
     3601 aagttcaagg tcctggggaa caccgaccgc cattccatca agaagaacct catcggcgct
     3661 ctcctgttcg acagcgggga gaccgctgag gctacgaggc tcaagagaac cgctaggcgc
     3721 cggtacacga gaaggaagaa caggatctgc tacctccaag agattttctc caacgagatg
     3781 gccaaggttg acgattcatt cttccaccgc ctggaggagt ctttcctcgt ggaggaggat
     3841 aagaagcacg agcggcatcc catcttcggc aacatcgtgg acgaggttgc ctaccacgag
     3901 aagtacccta cgatctacca tctgcggaag aagctcgtgg actccaccga taaggcggac
     3961 ctcagactga tctacctcgc tctggcccac atgatcaagt tccgcggcca tttcctgatc
     4021 gagggggatc tcaacccaga caacagcgat gttgacaagc tgttcatcca actcgtgcag
     4081 acctacaacc aactcttcga ggagaacccg atcaacgcct ctggcgtgga cgcgaaggct
     4141 atcctgtccg cgaggctctc gaagtccagg aggctggaga acctgatcgc tcagctccca
     4201 ggcgagaaga agaacggcct gttcgggaac ctcatcgctc tcagcctggg gctcaccccg
     4261 aacttcaagt cgaacttcga tctcgctgag gacgccaagc tgcaactctc caaggacacc
     4321 tacgacgatg acctcgataa cctcctggcc cagatcggcg atcaatacgc ggacctgttc
     4381 ctcgctgcca agaacctgtc ggacgccatc ctcctgtcag atatcctccg cgtgaacacc
     4441 gagatcacga aggctccact ctctgcctcc atgatcaagc gctacgacga gcaccatcag
     4501 gatctgaccc tcctgaaggc gctggtccgc caacagctcc cggagaagta caaggagatt
     4561 ttcttcgatc agtcgaagaa cggctacgct gggtacatcg acggcggggc ctcacaagag
     4621 gagttctaca agttcatcaa gccaatcctg gagaagatgg acggcacgga ggagctcctg
     4681 gtgaagctca acagggagga cctcctgcgg aagcagagaa ccttcgataa cggcagcatc
     4741 ccccaccaaa tccatctcgg ggagctgcac gccatcctga gaaggcaaga ggacttctac
     4801 cctttcctca aggataaccg ggagaagatc gagaagatcc tgaccttcag aatcccatac
     4861 tacgtcggcc ctctcgcgcg ggggaactca agattcgctt ggatgacccg caagtctgag
     4921 gagaccatca cgccgtggaa cttcgaggag gtggtggaca agggcgctag cgctcagtcg
     4981 ttcatcgaga ggatgaccaa cttcgacaag aacctgccca acgagaaggt gctccctaag
     5041 cactcgctcc tgtacgagta cttcaccgtc tacaacgagc tcacgaaggt gaagtacgtc
     5101 accgagggca tgcgcaagcc agcgttcctg tccggggagc agaagaaggc tatcgtggac
     5161 ctcctgttca agaccaaccg gaaggtcacg gttaagcaac tcaaggagga ctacttcaag
     5221 aagatcgagt gcttcgattc ggtcgagatc agcggcgttg aggaccgctt caacgccagc
     5281 ctcgggacct accacgatct cctgaagatc atcaaggata aggacttcct ggacaacgag
     5341 gagaacgagg atatcctgga ggacatcgtg ctgaccctca cgctgttcga ggacagggag
     5401 atgatcgagg agcgcctgaa gacgtacgcc catctcttcg atgacaaggt catgaagcaa
     5461 ctcaagcgcc ggagatacac cggctggggg aggctgtccc gcaagctcat caacggcatc
     5521 cgggacaagc agtccgggaa gaccatcctc gacttcctca agagcgatgg cttcgccaac
     5581 aggaacttca tgcaactgat ccacgatgac agcctcacct tcaaggagga tatccaaaag
     5641 gctcaagtga gcggccaggg ggactcgctg cacgagcata tcgcgaacct cgctggctcc
     5701 cccgcgatca agaagggcat cctccagacc gtgaaggttg tggacgagct cgtgaaggtc
     5761 atgggccggc acaagcctga gaacatcgtc atcgagatgg ccagagagaa ccaaaccacg
     5821 cagaaggggc aaaagaactc tagggagcgc atgaagcgca tcgaggaggg catcaaggag
     5881 ctggggtccc aaatcctcaa ggagcaccca gtggagaaca cccaactgca gaacgagaag
     5941 ctctacctgt actacctcca gaacggcagg gatatgtacg tggaccaaga gctggatatc
     6001 aaccgcctca gcgattacga cgtcgatcat atcgttcccc agtctttcct gaaggatgac
     6061 tccatcgaca acaaggtcct caccaggtcg gacaagaacc gcggcaagtc agataacgtt
     6121 ccatctgagg aggtcgttaa gaagatgaag aactactgga ggcagctcct gaacgccaag
     6181 ctgatcacgc aaaggaagtt cgacaacctc accaaggctg agagaggcgg gctctcagag
     6241 ctggacaagg ccggcttcat caagcggcag ctggtcgaga ccagacaaat cacgaagcac
     6301 gttgcgcaaa tcctcgactc tcggatgaac acgaagtacg atgagaacga caagctgatc
     6361 agggaggtta aggtgatcac cctgaagtct aagctcgtct ccgacttcag gaaggatttc
     6421 cagttctaca aggttcgcga gatcaacaac taccaccatg cccatgacgc ttacctcaac
     6481 gctgtggtcg gcaccgctct gatcaagaag tacccaaagc tggagtccga gttcgtgtac
     6541 ggggactaca aggtttacga tgtgcgcaag atgatcgcca agtcggagca agagatcggc
     6601 aaggctaccg ccaagtactt cttctactca aacatcatga acttcttcaa gaccgagatc
     6661 acgctggcca acggcgagat ccggaagaga ccgctcatcg agaccaacgg cgagacgggg
     6721 gagatcgtgt gggacaaggg cagggatttc gcgaccgtcc gcaaggttct ctccatgccc
     6781 caggtgaaca tcgtcaagaa gaccgaggtc caaacgggcg ggttctcaaa ggagtctatc
     6841 ctgcctaagc ggaacagcga caagctcatc gccagaaaga aggactggga cccaaagaag
     6901 tacggcgggt tcgacagccc taccgtggcc tactcggtcc tggttgtggc gaaggttgag
     6961 aagggcaagt ccaagaagct caagagcgtg aaggagctcc tggggatcac catcatggag
     7021 aggtccagct tcgagaagaa cccaatcgac ttcctggagg ccaagggcta caaggaggtg
     7081 aagaaggacc tgatcatcaa gctcccgaag tactctctct tcgagctgga gaacggcagg
     7141 aagagaatgc tggcttccgc tggcgagctc cagaagggga acgagctcgc gctgccaagc
     7201 aagtacgtga acttcctcta cctggcttcc cactacgaga agctcaaggg cagcccggag
     7261 gacaacgagc aaaagcagct gttcgtcgag cagcacaagc attacctcga cgagatcatc
     7321 gagcaaatct ccgagttcag caagcgcgtg atcctcgccg acgcgaacct ggataaggtc
     7381 ctctccgcct acaacaagca ccgggacaag cccatcagag agcaagcgga gaacatcatc
     7441 catctcttca ccctgacgaa cctcggcgct cctgctgctt tcaagtactt cgacaccacg
     7501 atcgatcgga agagatacac ctccacgaag gaggtcctgg acgcgaccct catccaccag
     7561 tcgatcaccg gcctgtacga gacgaggatc gacctctcac aactcggcgg ggataagaga
     7621 cccgcagcaa ccaagaaggc agggcaagca aagaagaaga agtgactcga gtatatgaag
     7681 atgaagatga aatatttggt gtgtcaaata aaaagcttgt gtgcttaagt ttgtgttttt
     7741 ttcttggctt gttgtgttat gaatttgtgg ctttttctaa tattaaatga atgtaagatc
     7801 acattataat gaataaacaa atgtttctat aatccattgt gaatgttttg ttggatctct
     7861 tctgcagcat ataactactg tatgtgctat ggtatggact atggaatatg attaaagata
     7921 aggagctcgt taaccggtga cggacgtggg caggtgtgat gaaaagcctt agggtcaggt
     7981 ggcacttttc ggggaaatgt gcgatgaacc cctatttgtt tatttttcta aatacattca
     8041 aatatgtatc cgctaatgag acaataaccc tgataaatgc ttcaattata ttgaaaaagg
     8101 aagagtatga gtatccaaca tttccgtgtc gcccttattc ccttttttgc ggccttctgc
     8161 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     8221 ggtgcccgag tgggttacat tgaactggat ctcaacagcg gcaaaatctt agagagtttt
     8281 cgccccgaag aacgcttccc aatgatgagc actttcaaag ttctgctatg tggcgcggtg
     8341 ttatcccgta ttgatgctgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     8401 gacttggttg agtattcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     8461 gaattatgta gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     8521 acgattggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     8581 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     8641 acgatgcctg tagcaatggc aacaactctc cgcaaactat taactggcga actacttact
     8701 cttgcttcac gccagcaact cattgactgg atggaggcgg acaaagttgc aggaccactt
     8761 ctgcgctcgg cacttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     8821 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     8881 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     8941 ggtgcctcac tgattaagca ttggtaactg tcagacgaag ggaaataaat agtagcccgc
     9001 ctgatgtgcg ggcttttttt tggtggtggt taccagtgtc gcttagctac cgtaggtcct
     9061 cttaggcggt ccgattagcg acatagtcat caat
//
