LOCUS       sequence-175553-       12678 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral 
            Replication: ToLCV.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12678)
  AUTHORS   Cermak T, Curtin SJ, Gil-Humanes J, Cegan R, Kono TJY, Konecna E, 
            Belanto JJ, Starker CG, Mathre JW, Greenstein RL, Voytas DF
  TITLE     A multi-purpose toolkit to enable advanced genome engineering in 
            plants.
  JOURNAL   Plant Cell. 2017 May 18. pii: tpc.00922.2016. doi: 
            10.1105/tpc.16.00922.
  PUBMED    28522548
REFERENCE   2  (bases 1 to 12678)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2017 May 18. pii: tpc.00922.2016. doi: 10.1105/tpc.16.00922."
COMMENT     Sequence Label: pTRANS_222
FEATURES             Location/Qualifiers
     source          1..12678
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1843..2640)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1954..1973)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2564..2583
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2703..3380)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    3571..3592
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3607..3612,3613..3630,3631..3637)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3645..3661
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3650..3672
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3669..3685
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3669..3685
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    4047..4171
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4196..4201,4202..4219,4220..4226)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4280..4960
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     CDS             5280..5585
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5464..5483
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5626..5750)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(5787..6039)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    7910..7934
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9234..9863
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10297..11364
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     rep_origin      11430..11624
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11860..11882)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11968..12108
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12023..12042
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12123..12140)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(12294..12678,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12374..12393)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgactctag ctagaggatc gatccgaacc ccagagtccc gctcagaaga actcgtcaag
     1861 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     1921 gcggtcagcc cattcgccgc caagttcttc agcaatatca cgggtagcca acgctatgtc
     1981 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     2041 ttccaccatg atattcggca agcaggcatc gccatgtgtc acgacgagat cctcgccgtc
     2101 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgttcttc
     2161 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     2221 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     2281 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     2341 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     2401 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctggag
     2461 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     2521 cagccgaaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     2581 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatccccat
     2641 ggtcgatcga cagatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
     2701 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg tcttgcgaag
     2761 gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct
     2821 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
     2881 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat
     2941 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg
     3001 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
     3061 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
     3121 ccatgttatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
     3181 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttgaa
     3241 cgatagcctt tcctttatcg caatgatggc atttgtaggt gccaccttcc ttttctactg
     3301 tccttttgat gaagtgacag atagctgggc aatggaatcc gaggaggttt cccgatatta
     3361 ccctttgttg aaaagtctca atagcccttt ggtcttctga gactgtatct ttgatattct
     3421 tggagtagac gagagtgtcg tgctccacca tgttggcaag ctgctctagc caatacgcaa
     3481 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     3541 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     3601 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     3661 atttcacaca ggaaacagct atgacatgat tacgaattcg agctcaaagt ttaacgcgtc
     3721 gattaagttg acttggtcaa tcggtgtctc tcaactctct ctatgtatcg gtgtattgga
     3781 gtcctatata tatggagact ctaatggcat aaatgtaaat atggaacttt aaatcaaaac
     3841 ccttacgctc caaaaagcgg ccatccgtat aatattaccg gatggccgcg attttttttt
     3901 tggaccccac aacgcaataa ctgacaaaga catgcggacc aatcacctga cgcgctcaaa
     3961 gcttaattgt tttgtgtttt ctatttaaac ttgggcacca agtattgtag ttcgtactac
     4021 aagtttgtac cgatcaaaag caggtgacaa gtttgtacaa aaaagctgaa cgagaaacgt
     4081 aaaatgatat aaatatcaat atattaaatt agattttgca taaaaaacag actacataat
     4141 actgtaaaac acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac
     4201 actttatgct tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc
     4261 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
     4321 ccaatggcat cgtaaagaac attttgaggc atttcagtca gtcgctcaat gtacctataa
     4381 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
     4441 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
     4501 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     4561 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     4621 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     4681 ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg tgagtttcac
     4741 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     4801 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     4861 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     4921 gtggcaggcg gggcgtaatc tagaggatcc ggcttactaa aagccagata acagtatgcg
     4981 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg
     5041 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc
     5101 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa
     5161 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga
     5221 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa
     5281 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac
     5341 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc
     5401 tgctgtcaga taaagtcccc cgtgaacttt acccggtggt gcatatcggg gatgaaagct
     5461 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg
     5521 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa
     5581 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata
     5641 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg
     5701 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt cacctgcaaa
     5761 aagtgtttga tcgccggcgg tacaaagtgg tagtaacata gatgacaccg cgcgcgataa
     5821 tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg
     5881 gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac
     5941 atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt
     6001 caatcttaag aaactttatt gccaaatgtt tgaacgatcg gggaaattcc taataaatat
     6061 taaattttat tatatgcgaa ctgtctacaa atgttgtttg cgtcaatgca tcccatagta
     6121 cataattcac tgctctaatt acattattca aactaattac acccaaatta ttaagaaatt
     6181 tcaacacttg tgttttaaat acccttaaga aacgaccagt cggaggctgt gaggtcatcc
     6241 agattcggta ggttaggaaa catttgtgta tccccaacac tttcctcagg ttgtgattga
     6301 actgtatccg gacggtgatg atgtcttcgt tcatcacgaa gggccggttt tggtgctctg
     6361 ttatcttgaa atatagggga ttttgaatct cccagataaa cacgccattc tctgcttgag
     6421 ctgcagtgat gagttcccct gtgcgtgaat ccatggtcgt ggcaggttat tgctatgaag
     6481 taagaacagc cacacggtag atcaactcgt cgacgtctgg tccccttctt ggctagcctg
     6541 tgctgtactt tgattggtac ctgagtagag tgggccttcg agggtgacga aggtcgcatt
     6601 ttttaaagcc cagtttttta gtgcagaatt cttttcctca tccaagtatt ctttatagct
     6661 ggagttgggt cctggattgc agaggaaggt agtgggaatt ccgcctttaa tttgaactgg
     6721 cttcccgtac tttgtatttg attgccagtc cctttgggcc cccatgaatt ctttaaagtg
     6781 ctttaggtag tgggggtcta cgtcatcaat gacgttgtac caggcatcat tactgtaaac
     6841 ctttgggcta aggtctagat gaccacacaa ataattatgt ggtcccaatg atctggccca
     6901 catagtcttc cccgtcctac tgtcaccctc aattactata cttatgggtc tcagaggccg
     6961 cgcagcggga ctgcagacgt tctcggcagc ccactcttca agttcttctg gaacttggtc
     7021 gaaagaagaa gataaaaaag gtgaaacata aacatccatt ggaggtgtaa aaatcctatc
     7081 taaattagaa tttaaattgt gaaattgtaa aacaaaatct ttgggagctt tctccctcaa
     7141 tatattaagg gcctgagctt tggaccctga attgattgcc tcggcatatg cgtcgttggc
     7201 agattggcaa cctcctctag ctgatcgtcc atcgacttgg aaaactccat gatcaatgac
     7261 gtctccgtct ttttccatgt aggatttgac atcgcttgag ctcttagctc cctgaatgtt
     7321 cggatggaaa tgtgctgacc tggttgggga tgtgaggtcg aagaatctat tgtttttgca
     7381 ctggaacttt ccttcgaact ggatgagcac atgcaagtga ggagtcccat cttcgtgaag
     7441 ctctctgcaa attctaataa attttttgaa agtgggtgtt tggagattta ataatttggg
     7501 aaagtgctct tctttagtta gagagcactt gggataagtg agaaaataat ttttggcatt
     7561 tattttaaag cgattggggg ctgccatgtt gacttggtca atcggtgtct ctcaactctc
     7621 tctatgtatc ggtgtattgg agtcctatat atatggagac tctaatggca taaatgtaaa
     7681 tatggaactt taaatcaaaa cccttacgct ccaaaaagcg gccatccgta taatattacc
     7741 ggatggccgc gatttttttt ttggacccca caacgcaata actgacaaag acatgcggac
     7801 caatcacctg acgcgctcaa agcttaattg ttttgtgttt tctatttaaa cttgggcacc
     7861 aagtattgta gttcgtactt ggggccctcg agtttaaact atcagtgttt gacaggatat
     7921 attggcgggt aaacctaaga gaaaagagcg tttattagaa taacggatat ttaaaagggc
     7981 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
     8041 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
     8101 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
     8161 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
     8221 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
     8281 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
     8341 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
     8401 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
     8461 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
     8521 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
     8581 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
     8641 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
     8701 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
     8761 agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg taccgcgcac ttgagcgcag
     8821 cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc ttccgtgagg acgcattgac
     8881 cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa gaggaacaag catgaaaccg
     8941 caccaggacg gccaggacga accgtttttc attaccgaag agatcgaggc ggagatgatc
     9001 gcggccgggt acgtgttcga gccgcccgcg cacggctcaa ccgtgcggct gcatgaaatc
     9061 ctggccggtt tgtctgatgc caagctggcg gcctggccgg ccagcttggc cgctgaagaa
     9121 accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt aaaacagctt gcgtcatgcg
     9181 gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac gcaaggggaa cgcatgaagg
     9241 ttatcgctgt acttaaccag aaaggcgggt caggcaagac gaccatcgca acccatctag
     9301 cccgcgccct gcaactcgcc ggggccgatg ttctgttagt cgattccgat ccccagggca
     9361 gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct aaccgttgtc ggcatcgacc
     9421 gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg cgacttcgta gtgatcgacg
     9481 gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa ggcagccgac ttcgtgctga
     9541 ttccggtgca gccaagccct tacgacatat gggccaccgc cgacctggtg gagctggtta
     9601 agcagcgcat tgaggtcacg gatggaaggc tacaagcggc ctttgtcgtg tcgcgggcga
     9661 tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct ggccgggtac gagctgccca
     9721 ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg cactgccgcc gccggcacaa
     9781 ccgttcttga atcagaaccc gagggcgacg ctgcccgcga ggtccaggcg ctggccgctg
     9841 aaattaaatc aaaactcatt tgagttaatg aggtaaagag aaaatgagca aaagcacaaa
     9901 cacgctaagt gccggccgtc cgagcgcacg cagcagcaag gctgcaacgt tggccagcct
     9961 ggcagacacg ccagccatga agcgggtcaa ctttcagttg ccggcggagg atcacaccaa
    10021 gctgaagatg tacgcggtac gccaaggcaa gaccattacc gagctgctat ctgaatacat
    10081 cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag tagatgaatt ttagcggcta
    10141 aaggaggcgg catggaaaat caagaacaac caggcaccga cgccgtggaa tgccccatgt
    10201 gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt tgtctgccgg ccctgcaatg
    10261 gcactggaac ccccaagccc gaggaatcgg cgtgacggtc gcaaaccatc cggcccggta
    10321 caaatcggcg cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc
    10381 cagcggcaac gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat
    10441 cgaatccgca aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg
    10501 cccaagggcg acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc
    10561 gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct
    10621 ggcgaggtga tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc
    10681 ggcatggcca gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa
    10741 tccatgaacc gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac
    10801 gttgcggacg tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg
    10861 gtagaaacct gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc
    10921 aagaacggcc gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc
    10981 gtaaagagcg aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac
    11041 cgcgagatca cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg
    11101 atcgatcccg gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca
    11161 gaagccagat ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag
    11221 aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg
    11281 aaggaggagg cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag
    11341 ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca
    11401 ggggaaaaag gtcgaaaagg cctctttcct gtggatagca cgtacattgg gaacccaaag
    11461 ccgtacattg ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg
    11521 tcacacatgt aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct
    11581 ttaaaactta ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc
    11641 acagcccaag agctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc
    11701 gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc
    11761 aatctaccag ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa
    11821 ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc
    11881 ggaaacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
    11941 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg
    12001 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
    12061 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcc ctcttccgct
    12121 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    12181 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    12241 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    12301 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    12361 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    12421 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    12481 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    12541 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    12601 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    12661 attagcagag cgaggtat
//
