LOCUS       sequence-17562-e         691 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 691)
  TITLE     Viral vectors.
REFERENCE   2  (bases 1 to 691)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMKO.1 puro PTEN shRNA
FEATURES             Location/Qualifiers
     source          1..691
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        56..304
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     56..76
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     227..246
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(388..408)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tcactccttc tctaggcgcc ggaattgaag atctgtcgac aaggtcgggc aggaagaggg
       61 cctatttccc atgattcctt catatttgca tatacgatac aaggctgtta gagagataat
      121 tagaattaat ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta
      181 ataatttctt gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct
      241 taccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa
      301 caccggactt gaaggcgtat acaggactcg agtcctgtat acgccttcaa gtctttttga
      361 attcgccagc acagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc
      421 caggcttccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt
      481 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
      541 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
      601 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
      661 cggcctctga gctattccag aagtagtgag g
//
