LOCUS       sequence-17564-e         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Boehm JS, Hession MT, Bulmer SE, Hahn WC
  TITLE     Transformation of human and murine fibroblasts without viral 
            oncoproteins.
  JOURNAL   Mol Cell Biol. 2005 Aug . 25(15):6464-74.
  PUBMED    16024784
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2005 Aug . 25(15):6464-74."
COMMENT     Sequence Label: pBabe GFP Small T antigen
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             3..521
                     /codon_start=1
                     /product="SV40 (simian virus 40) small t antigen"
                     /label=small t antigen
                     /translation="KVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDKGG
                     DEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECAKK
                     MSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDIIG
                     QTTYRDLKL"
     primer_bind     complement(551..571)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga
       61 gtgcctgggg gaatattcct ctgatgagaa aggcatattt aaaaaaatgc aaggagtttc
      121 atcctgataa aggaggagat gaagaaaaaa tgaagaaaat gaatactctg tacaagaaaa
      181 tggaagatgg agtaaaatat gctcatcaac ctgactttgg aggcttctgg gatgcaactg
      241 aggtatttgc ttcttcctta aatcctggtg ttgatgcaat gtactgcaaa caatggcctg
      301 agtgtgcaaa gaaaatgtct gctaactgca tatgcttgct gtgcttactg aggatgaagc
      361 atgaaaatag aaaattatac aggaaagatc cacttgtgtg ggttgattgc tactgcttcg
      421 attgctttag aatgtggttt ggacttgatc tttgtgaagg aaccttactt ctgtggtgtg
      481 acataattgg acaaactacc tacagagatt taaagctcta agaattcgcc agcacagtgg
      541 tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
      601 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
      661 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
      721 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
      781 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
      841 cagaagtagt gaggaggct
//
