LOCUS       sequence-175667-        1134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Ubl4A (90-107 residues) tagged with FLAG-EGFP at N- 
            terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pEGFP-C1-FLAG-GFP-Ubl4A-H1(90-107)
FEATURES             Location/Qualifiers
     source          1..1134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    228..349
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(265..284)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     319..338
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(356..811)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(493..514)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     705..724
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        838..942
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        944..1134
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(967..987)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 acaagtccgg actcagatct tccccacccc cgcaggtctg gcagctgatc tccaaagtct
       61 tggcccgcca cttctaggtc gacggtaccg cgggcccggg atccaccgga tctagataac
      121 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
      181 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
      241 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
      301 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt
      361 gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
      421 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
      481 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
      541 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
      601 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
      661 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
      721 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
      781 gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc
      841 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
      901 taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc ggaaagaacc
      961 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     1021 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     1081 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcc
//
