LOCUS       sequence-175669-        1196 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Ubl4A (108-130 residues) tagged with FLAG-EGFP at N- 
            terminus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 1196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: pEGFP-C1-FLAG-GFP-Ubl4A-H2(108-130)
FEATURES             Location/Qualifiers
     source          1..1196
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    239..360
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(276..295)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     330..349
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(367..822)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(504..525)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     716..735
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        849..953
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        955..1146
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(978..998)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtccggactc agatctagtg cggcagatgc cagcagggtc ctggaacagc tacagaggga
       61 ttacgagagg tccctgagtc gcctgtaggt cgacggtacc gcgggcccgg gatccaccgg
      121 atctagataa ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
      181 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
      241 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
      301 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
      361 acgcgtaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
      421 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      481 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      541 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
      601 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
      661 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
      721 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
      781 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
      841 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
      901 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtcctgagg
      961 cggaaagaac cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     1021 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     1081 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     1141 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccatt
//
