LOCUS       sequence-17567-e         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Boehm JS, Hession MT, Bulmer SE, Hahn WC
  TITLE     Transformation of human and murine fibroblasts without viral 
            oncoproteins.
  JOURNAL   Mol Cell Biol. 2005 Aug . 25(15):6464-74.
  PUBMED    16024784
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2005 Aug . 25(15):6464-74."
COMMENT     Sequence Label: pBabe HcRed
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(35..55)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        40..369
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      220..355
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      401..410
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acgtaggaat tcccagcaca gtggtcgacc ctgtggaatg tgtgtcagtt agggtgtgga
       61 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      121 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
      181 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
      241 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
      301 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
      361 ttttgcaaaa agcttggtac cgagctcgga tccaccggtc gccaccatgg tgagcggcct
      421 gctgaaggag agtatgcgca tcaagatgta catggagggc accgtgaacg gccactactt
      481 caagtgcgag ggcgagggcg acggcaaccc cttcgccggc acccagagca tgagaatcca
      541 cgtgaccgag ggcgcccccc tgcccttcgc cttcgacatc ctggccccct gctgcgagta
      601 cggcagcagg accttcgtgc accacaccgc cgagatcccc gacttcttca agcagagctt
      661 ccccgagggc ttcacctggg agagaaccac cacctacgag gacggcggca tcctgaccgc
      721 ccaccaggac accagcctgg agggcaactg cctgatctac aaggtgaagg tgcacggcac
      781 caacttcccc gccgacggcc ccgtgatga
//
