LOCUS       sequence-175670-       10207 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for creation of CRISPR/Cas9-mediated Bxb1 attP-flanked 
            alleles..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Voutev R, Mann RS
  TITLE     Bxb1 phage recombinase assists genome engineering in Drosophila 
            melanogaster.
  JOURNAL   Biotechniques. 2017 Jan 1;62(1):37-38. doi: 10.2144/000114494.
  PUBMED    28118814
REFERENCE   2  (bases 1 to 10207)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2017-01-1- 1"; volume: "62"; issue: "1"; 
            pages: "37-38"
COMMENT     Sequence Label: pRVV651
FEATURES             Location/Qualifiers
     source          1..10207
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(674..692)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1144..1215,1617..1890,1965..2619,2681..2996,3200..3331,
                     3402..4016)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4844..4891
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     CDS             join(6799..6801,6802..6804,6805..7518)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6844..6865)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7105..7124)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7452..7473
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7540..7661
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7551..7570)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7605..7624
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    7768..7815
                     /label=Bxb1 attP
                     /bound_moiety="Mycobacteriophage Bxb1 integrase"
                     /note="Minimal attP site for the Mycopbacteriophage Bxb1 
                     integrase (Voutev and Mann, 2017)"
     promoter        complement(8019..8037)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8058..8074)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8058..8074)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8071..8093)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8082..8098
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8106..8112,8113..8130,8131..8136))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8151..8172
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8289..8306)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8460..9048)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8540..8559)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9219..10010,10011..10079))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9842..9861
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10080..10184)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggccctagta tgtatgtaag ttaataaaac ccatttttgc ggaaagtaga taaaaaaaac
      721 attttttttt tttactgcac tggatatcat tgaacttatc tgatcagttt taaatttact
      781 tcgatccaag ggtatttgat gtaccaggtt ctttcgatta cctctcactc aaaatgacat
      841 tccactcaaa gtcagcgctg tttgcctcct tctctgtcca cagaaatatc gccgtctctt
      901 tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag cgttacgtag cgtcaatgtc
      961 cgccttcagt tgcattttgt cagcggtttc gtgacgaagc tccaagcggt ttacgccatc
     1021 aattaaacac aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt ttgttttttg
     1081 agtgattggg gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg aaaaatcccg
     1141 gcaatgggcc aagaggatca ggagctatta attcgcggag gcagcaaaca cccatctgcc
     1201 gagcatctga acaatgtgag tagtacatgt gcatacatct taagttcact tgatctatag
     1261 gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca caaaaatccc
     1321 aaaccgcaat tgcacaaaca aatagtgaca cgaaacagat tattctggta gctgttctcg
     1381 ctatataaga caatttttga gatcatatca tgatcaagac atctaaaggc attcattttc
     1441 gactatattc ttttttacaa aaaatataac aaccagatat tttaagctga tcctagatgc
     1501 acaaaaaata aataaaagta taaacctact tcgtaggata cttcggggta ctttttgttc
     1561 ggggttagat gagcataacg cttgtagttg atatttgaga tcccctatca ttgcagggtg
     1621 acagcggagc ggcttcgcag agctgcatta accagggctt cgggcaggcc aaaaactacg
     1681 gcacgctccg gccacccagt ccgccggagg actccggttc agggagcggc caactagccg
     1741 agaacctcac ctatgcctgg cacaatatgg acatctttgg ggcggtcaat cagccgggct
     1801 ccggatggcg gcagctggtc aaccggacac gcggactatt ctgcaacgag cgacacatac
     1861 cggcgcccag gaaacatttg ctcaagaacg gtgagtttct attcgcagtc ggctgatctg
     1921 tgtgaaatct taataaaggg tccaattacc aatttgaaac tcagtttgcg gcgtggccta
     1981 tccgggcgaa cttttggccg tgatgggcag ttccggtgcc ggaaagacga ccctgctgaa
     2041 tgcccttgcc tttcgatcgc cgcagggcat ccaagtatcg ccatccggga tgcgactgct
     2101 caatggccaa cctgtggacg ccaaggagat gcaggccagg tgcgcctatg tccagcagga
     2161 tgacctcttt atcggctccc taacggccag ggaacacctg attttccaag ccatggtgcg
     2221 gatgccacga catctgacct atcggcagcg agtggcccgc gtggatcagg tgatccagga
     2281 gctttcgctc agcaaatgtc agcacacgat catcggtgtg cccggcaggg tgaaaggtct
     2341 gtccggcgga gaaaggaagc gtctggcatt cgcctccgag gctctaaccg atccgccgct
     2401 tctgatctgc gatgagccca cctccggact ggactccttt accgcccaca gcgtcgtcca
     2461 ggtgctgaag aagctgtcgc agaagggcaa gaccgtcatc ctgaccattc atcagccgtc
     2521 ttccgagctg tttgagctct ttgacaagat ccttctgatg gccgagggca gggtagcttt
     2581 cttgggcact cccagcgaag ccgtcgactt cttttcctag tgagttcgat gtgtttatta
     2641 agggtatcta gtattacata acatctcaac tcctatccag cgtgggtgcc cagtgtccta
     2701 ccaactacaa tccggcggac ttttacgtac aggtgttggc cgttgtgccc ggacgggaga
     2761 tcgagtcccg tgatcggatc gccaagatat gcgacaattt tgccattagc aaagtagccc
     2821 gggatatgga gcagttgttg gccaccaaaa atctggagaa gccactggag cagccggaga
     2881 atgggtacac ctacaaggcc acctggttca tgcagttccg ggcggtcctg tggcgatcct
     2941 ggctgtcggt gctcaaggaa ccactcctcg taaaagtgcg acttattcag acaacggtga
     3001 gtggttccag tggaaacaaa tgatataacg cttacaattc ttggaaacaa attcgctaga
     3061 ttttagatag aattgcctga ttccacaccc ttcttagttt ttttcaatga gatgtatagt
     3121 ttatagtttt gcagaagata aataaatttc atttaactcg cgaatattaa tgagatgcga
     3181 gtaacatttt aatttgcaga tggttgccat cttgattggc ctcatctttt tgggccaaca
     3241 actcacgcaa gtgggtgtga tgaatatcaa cggagccatc ttcctcttcc tgaccaacat
     3301 gacctttcaa aacgtctttg ccacgataaa tgtaagtcat gtttagaata catttgcatt
     3361 tcaataattt actaactttc taatgaatcg attcgattta ggtgttcacc tcagagctgc
     3421 cagtttttat gagggaggcc cgaagtcgac tttatcgctg tgacacatac tttctgggca
     3481 aaacgattgc cgaattgccg ctttttctca cagtgccact ggtcttcacg gcgattgcct
     3541 atccgatgat cggactgcgg gccggagtgc tgcacttctt caactgcctg gcgctggtca
     3601 ctctggtggc caatgtgtca acgtccttcg gatatctaat atcctgcgcc agctcctcga
     3661 cctcgatggc gctgtctgtg ggtccgccgg ttatcatacc attcctgctc tttggcggct
     3721 tcttcttgaa ctcgggctcg gtgccagtat acctcaaatg gttgtcgtac ctctcatggt
     3781 tccgttacgc caacgagggt ctgctgatta accaatgggc ggacgtggag ccgggcgaaa
     3841 ttagctgcac atcgtcgaac accacgtgcc ccagttcggg caaggtcatc ctggagacgc
     3901 ttaacttctc cgccgccgat ctgccgctgg actacgtggg tctggccatt ctcatcgtga
     3961 gcttccgggt gctcgcatat ctggctctaa gacttcgggc ccgacgcaag gagtagccga
     4021 catatatccg aaataactgc ttgttttttt tttttaccat tattaccatc gtgtttactg
     4081 tttattgccc cctcaaaaag ctaatgtaat tatatttgtg ccaataaaaa caagatatga
     4141 cctatagaat acaagtattt ccccttcgaa catccccaca agtagacttt ggatttgtct
     4201 tctaaccaaa agacttacac acctgcatac cttacatcaa aaactcgttt atcgctacat
     4261 aaaacaccgg gatatatttt ttatatacat acttttcaaa tcgcgcgccc tcttcataat
     4321 tcacctccac cacaccacgt ttcgtagttg ctctttcgct gtctcccacc cgctctccgc
     4381 aacacattca ccttttgttc gacgaccttg gagcgactgt cgttagttcc gcgcgattcg
     4441 gttcgctcaa atggttccga gtggttcatt tcgtctcaat agaaattagt aataaatatt
     4501 tgtatgtaca atttatttgc tccaatatat ttgtatatat ttccctcaca gctatattta
     4561 ttctaattta atattatgac tttttaaggt aattttttgt gacctgttcg gagtgattag
     4621 cgttacaatt tgaactgaaa gtgacatcca gtgtttgttc cttgtgtaga tgcatctcaa
     4681 aaaaatggtg ggcataatag tgttgtttat atatatcaaa aataacaact ataataataa
     4741 gaatacattt aatttagaaa atgcttggat ttcactggaa ctaggctaga tttaaatgca
     4801 tgccctgcag gaagcttcct aggaggccta gatctgcggc cgcggtttgt accgtacacc
     4861 actgagaccg cggtggttga ccagacaaac cttaattaag aagttcctat tcttcaaaag
     4921 gtataggaac ttctctagcc cggcttgctg ttcttcgcgt tcaaaatctc cagctccatt
     4981 ttgctttcgg tgcgcttgca atcagtactg tccaaaatcg aaaatcgccg aaccgtagtg
     5041 tgacgtgcgg ggctctgcga aaataaactt ttttaggtat atggccacac acggggaaag
     5101 cacagtggat tatatgtttt aatattataa tatgcaggtt ttcattactt atccagatgt
     5161 aagcccactt aaagcgattt aacaattatt tgccgaaaga gtataaacaa atttcactta
     5221 aaaaatggat taagaaaagc ttgtgtaaga ttatgcgcag cgttgccaga tagctccatt
     5281 taaaacactt caaaaacaat aagttttgaa aatatataca taaatagcag tcgttgccgc
     5341 aacgctcaac acatcacact tttaaaacac cctttaccta cacagaatta ctttttaaat
     5401 ttccagtcaa gctgcgagtt tcaaaattat agccggtaga gaagacagtg ctatttcaaa
     5461 agcaaactaa caagggtctt aaattccaaa acaccaatcc taacaagcct tggacttttg
     5521 taagtttaga tcaaaggtgg cattgcattc aatgtcatgg taagaagtag gtcgtctagg
     5581 tagaaatcct cattcagccg gtcaagtcag tacgagaaag gtctcaattt gaaattgtct
     5641 taaaaatatt ttattgtttt gtactgtggt gagtttaaac gaaaaacaca aaaaaaaagt
     5701 gatacacaga aatcataaaa aattttaata caaggtattc gtacgtatca aaaacatttc
     5761 ggcacaattt tttttctctg tactaaagtg ttacgaacac tacggtattt tttagtgatt
     5821 ttcaacggac accgaaggta tataaacagc gttcgcgaac ggtcgccttc aaaaccaatt
     5881 gacatttgca gcagcaagta caagcagaaa gtaaagcgca atcagcgaaa aatttatact
     5941 taattgttgg tgattaaagt acaattaaaa gaacattctc gaaagtcaca agaaacgtaa
     6001 gtttttaact cgctgttacc aattagtaat aagagcaaca agacgttgag taatttcaag
     6061 aaaaactgca tttcaaggtc tttgttcggc catttttttt ttattcaacg ctctacgtaa
     6121 ttacaaaata agaaattggc agccacgcat cttgttttcc caatcaattg gcatcaaaac
     6181 gcaaacaaat ctataaataa aacttgcgtg ttgattttcg ccaagattta ttggcaaatt
     6241 gtgaaattcg cagtgacgca tttgaaaatt cgagaaatca cgaacgcact cgagcatttg
     6301 tgtgcatgtt attagttagt tagttctttg cttaattgaa gtattttacc aacgaaatcc
     6361 acttattttt agctgaaata gagtaggttg cttgaaacga aagccacgtc tggaaaattt
     6421 cttattgctt agtagttgtg acgtcaccat atacacacaa aataatgtgt atgcatgcgt
     6481 ttcagctgtg tatatataca tgcacacact cgcattatga aaacgatgac gagcaacgga
     6541 acaggtttct caactacctt tgttcctgtt tcttcgcttt cctttgttcc aatattcgta
     6601 gagggttaat aggggtttct caacaaagtt ggcgtcgata aataagtttc ccatttttat
     6661 tccccagcca ggaagttagt ttcaatagtt ttgtaatttc aacgaaactc atttgatttc
     6721 gtactaattt tccacatctc tattttctgc ccgcagaata atccaagggc ggatcccccg
     6781 ggctgcagga attcaaaaat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     6841 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     6901 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     6961 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     7021 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     7081 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     7141 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     7201 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     7261 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac
     7321 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     7381 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     7441 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     7501 gacgagctgt acaagtaagg tacctctaga cagacatgat aagatacatt gatgagtttg
     7561 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     7621 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     7681 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcgaa gttcctattc
     7741 ttcaaaaggt ataggaactt caccggtggt ttgtctggtc aaccaccgcg gtctcagtgg
     7801 tgtacggtac aaaccggcgc gccgctagcc atatggggat tggtggcgac gactcctgga
     7861 gcccgtcagt atcggcggaa ttacagctga gcgccggtcg ctaccattac cagttggtct
     7921 ggtgtcaaaa ataataataa ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat
     7981 ccctcgaggg tacctctagt tctagccagc ttttgttccc tttagtgagg gttaatttcg
     8041 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     8101 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     8161 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     8221 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     8281 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     8341 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     8401 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     8461 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     8521 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     8581 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     8641 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     8701 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     8761 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     8821 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     8881 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     8941 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     9001 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     9061 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     9121 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     9181 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     9241 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     9301 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     9361 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     9421 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     9481 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     9541 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     9601 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     9661 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     9721 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     9781 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     9841 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     9901 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     9961 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
    10021 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
    10081 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
    10141 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
    10201 gtgccac
//
