LOCUS       sequence-175680-        6102 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hybrid McpC[1-350]-Tar[267-553] expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Bi S, Pollard AM, Yang Y, Jin F, Sourjik V
  TITLE     Engineering Hybrid Chemotaxis Receptors in Bacteria.
  JOURNAL   ACS Synth Biol. 2016 Jun 10.
  PUBMED    27285081
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 Jun 10."
COMMENT     Sequence Label: pSB3
FEATURES             Location/Qualifiers
     source          1..6102
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      305..339
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(758..1417)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1332..1351
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1418..1520)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(1912..1929)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2046..2591)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(2126..2145)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtttcggaaa tggatcgcgt cacgcaacag aacgcatcgc tggtgcagga atcagctgcc
       61 gccgccgctg cgctggaaga acaggcgagt cgtttaacgc aagcagtttc cgcgttccgt
      121 ctggcagcca gcccactcac caataaaccg caaacaccat cccgtcctgc cagtgagcaa
      181 ccaccggctc agccacgact gcgaattgct gaacaagatc caaactggga aacattttga
      241 ggatccgcgg ataaataagt aacgatccgg tccagtaatg acctcagaac tccatctgga
      301 tttgttcaga acgctcggtt gccgccgggc gttttttatt ggtgagaatc gcagcaactt
      361 gtcgcgccaa tcgagccatg tcgtcgtcaa cgacccccca ttcaagaaca gcaagcagca
      421 ttgagaactt tggaatccag tccctcttcc acctgctgac cggatcagca gtccccggaa
      481 catcgtagct gacgccttcg cgttgctcag ttgtccaacc ccggaaacgg gaaaaagcaa
      541 gttttccccg ctcccggcgt ttcaataact gaaaaccata ctatttcaca gtttaaatca
      601 cattaaacga cagtaatccc cgttgatttg tgcgccaaca cagatccgtc gaccgggtcg
      661 aatttgcttt cgaatttctg ccattcatcc gcttattatc acttattcag gcgtagcaac
      721 caggcgttta agggcaccaa taactgcctt aaaaaaatta cgccccgccc tgccactcat
      781 cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca caaacggcat
      841 gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa tatttgccca
      901 tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca aaactggtga
      961 aactcaccca gggattggct gagacgaaaa acatattctc aataaaccct ttagggaaat
     1021 aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga aactgccgga
     1081 aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca tggaaaacgg
     1141 tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt gccatacgga
     1201 attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga taaaacttgt
     1261 gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg gtctggttat
     1321 aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc cattgggata
     1381 tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta gctcctgaaa
     1441 atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg tgaaagttgg
     1501 aacctcttac gtgccgatca acgtctcatt ttcgccaaaa gttggcccag ggcttcccgg
     1561 tatcaacagg gacaccagga tttatttatt ctgcgaagtg atcttccgtc acaggtattt
     1621 attcggcgca aagtgcgtcg ggtgatgctg ccaacttact gatttagtgt atgatggtgt
     1681 ttttgaggtg ctccagtggc ttctgtttct atcagctgtc cctcctgttc agctactgac
     1741 ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctagcggag tgtatactgg
     1801 cttactatgt tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa
     1861 ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac
     1921 tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg
     1981 agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag
     2041 ccgtttttcc ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag
     2101 tggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc
     2161 gtgcgctctc ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt
     2221 ttgtctcatt ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg
     2281 tatgcacgaa ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga
     2341 gtccaacccg gaaagacatg caaaagcacc actggcagca gccactggta attgatttag
     2401 aggagttagt cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg
     2461 actgcgctcc tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg
     2521 aaaaaccgcc ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa
     2581 aacgatctca agaagatcat cttattaatc agataaaata tttctagatt tcagtgcaat
     2641 ttatctcttc aaatgtagca cctgaagtca gccccatacg atataagttg taattctcat
     2701 gtttgacagc ttatcatcga tcccgcaaga ggcccggcag taccggcata accaagccta
     2761 tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca
     2821 tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttct
     2881 tgcgaaacag ctctcgacca ggtttatact ggtcgtgcag atgaaagcag tttttgccga
     2941 ggtcgattcc gaccagcgcc atatcgctca tagtgatggt ctccgaatta taaacaccct
     3001 gcgaaagcgt agcctacgca gggtgtcggg tgctcatccc attacccgtt ttcccatctc
     3061 ttaacttaag gggggaacgg tgtcctgtct ttcatggggc tcgttcactg ctaacttacc
     3121 tgcttccctg tctgtcaatt ctctctatcc tgcgatcccg cgaagaacca aaaaagctcg
     3181 acagagggcg cggtcatttt aggtcgggcg gatcggcgcc gccggctcgg ctggtgtgcc
     3241 gcacagcacc gcctacgtga gctgccagtt gatgaacttc ccccgttgcc agctagggcg
     3301 caagcgggct gtataagatc actgcccatc acattgatcg gctcggattt tttctcaatc
     3361 cgtaaacagg tcaaacatca gttgccgcaa ccaaatattg gctaggtcct tgtggtactt
     3421 cgcatgccag aacatgttga tggctatttc aggcaagacg actgggtgcg gcaaggcgct
     3481 taggccgaag ggctccacac agcagtcggc taaacgtatc ggcacagtgg cgagcagatc
     3541 ggtgcgctgg aggatgtggc caacggcggc gaagtgcggc acttccagac ggatgtcgcg
     3601 ccggatgccg acccgtgtca tgtacgtgtc cacctcgccg tggccggtgc cagcggcgat
     3661 gacacgcacg tggccgtagg aacagaagcg ctccagagtc aggggttcgc gggtgactgg
     3721 atggtccttg cgacataggc acacgtagtg attctggagc agccggcgct gaaagaagcc
     3781 agtttgcaga ttgggaagca ggcccacggc caagtccacg gttccgttct gcaaggcctg
     3841 catcaggctc atcgaactgt cgcgcaccgt actgatcacg caattggggg cctggtgagc
     3901 cagcacatcc atcagccgcg gcatgaagta gatctcgcca atgtcggtca tggccagggt
     3961 gaaggtacgc tcgctggtca gcggatcgaa gctttcatgg tgctgtaggg cgttgcgcag
     4021 tgcgtgcatg gccgaagtga cgggctcggc cagatgcgcg gcatagggtg tgggttccat
     4081 tccctgatgt gtgcgcacga agagtgggtc ctgtagcgag gtgcgcaggc gtttcagcgc
     4141 attgctcacg gcaggctggg tcaggcccag gttctccgca gtgatagaga cgcgtctgtc
     4201 gaccagcaac tggttgaaca ccaccagcag gtttaaatcc aggtcacgca gttccatggg
     4261 gcctcgcttg ggttattgct ggtgcccggc cgggcgcaat attcatgttg atgatttatt
     4321 atatatcgag tggtgtattt atcaatattg tttgctccgt tatcgttatt aacaagtcat
     4381 caataatgcc ataaggagta ccatatgcat gatatctgca gcggccgcat gtttaaaaaa
     4441 cttcacatga agattgctgt ttttgtttcg attatgttaa tcatcacagt tgttctctta
     4501 atgctgtctt cttatttgac gctaaaaccg atgattacag aggatgggaa aaatacaact
     4561 caaaacgtga cgcagtcact tgaacaaaat attgaattgc aattaaagag ttatgccatt
     4621 tcgttatcaa gattggctaa tggggaatta acgcatacat ttgtaacgaa accaagtaaa
     4681 gaagcttctc ggctgtttca cgatgacatt aagcaaatca aagacaacga tgattatgtt
     4741 gcgatggctt acataggcac cgcaaaaaaa gaaatgttta catatccaaa agcggatttc
     4801 gctgaggatt acgatccaac atcaagacca tggtataagc ttgcagctga aaccccggat
     4861 caagttgttt ggacggaacc ttataaagat gtcgtgacag gagacatgat tgttactgca
     4921 tcgaaagcga tcttagacag gcaaaaagtg ataggggttg ccagctatga cttgaaactc
     4981 tctgctatcc agagtatggt caataaacaa aaggtgcctt acaaaggatt tgccttttta
     5041 gctgatgcga gcggcaatct tttagcccat ccgtcaaatc aaggaaaaaa tatatcaaag
     5101 gatcaaacac tgcaaaccat cgcatccgag aaaaaaggga tacaagatgt gaatgggaaa
     5161 atggttgtgt accaaacgat tggcgagaca ggctggaagg tcggaacgca atttgatacc
     5221 gatcaattaa tgtggatttc agataaaatg aatcgggcca atctttggat atcattgatt
     5281 gcgcttatca taacaattat tctcagctac ttcctggcca aaacgataac cggtccgata
     5341 cagcagctga tcgtaaaaac gaaagctgtt tccgcaggcg atttaacagt gcgcgcggaa
     5401 tcaaaatcta aggatgaagt cggtatttta acaagagatt tcaatctgat ggttgaaaat
     5461 atgaaggaaa tggttgagca tgtccgcgaa ggttcagatg ccatctatgc cggtacccgt
     5521 gaaattgcgg cgggcaacac cgatctttcc tcccgtactg aacagcaggc atccgcgctg
     5581 gaagaaactg ccgccagcat ggagcagctc accgcgacag tgaagcaaaa cgccgataac
     5641 gcccgccagg cctcgcaact ggcgcaaagt gcctccgaca ccgcccagca cggcggcaaa
     5701 gtggtggatg gcgtagtgaa aacgatgcat gagatcgccg atagttcgaa gaaaattgcc
     5761 gacattatca gcgttatcga cggtattgcc ttccagacta atatcctcgc gctgaatgcc
     5821 gcggttgaag ccgcgcgtgc gggtgaacag ggccgtggtt ttgccgtggt ggcgggtgaa
     5881 gtgcgtaatc ttgccagtcg cagcgcccag gcggcaaaag agatcaaagc cctcattgaa
     5941 gactccgtct cacgcgttga taccggttcg gtgctggtcg aaagcgccgg ggaaacaatg
     6001 aacaatatcg tcaatgctgt cactcgcgtg actgacatta tgggcgagat tgcatcggca
     6061 tcggatgaac agagccgtgg catcgatcaa gtcgcattgg cg
//
