LOCUS       sequence-175705-        9050 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  The pMaRS plasmid is the same construct as pMaRSL274G, but contains 
            a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9050)
  AUTHORS   Mahdavi A, Hamblin GD, Jindal GA, Bagert JD, Dong C, Sweredoski MJ, 
            Hess S, Schuman EM, Tirrell DA
  TITLE     Engineered Aminoacyl-tRNA Synthetase for Cell-Selective Analysis of 
            Mammalian Protein Synthesis.
  JOURNAL   J Am Chem Soc. 2016 Apr 6;138(13):4278-81. doi: 
            10.1021/jacs.5b08980. Epub 2016 Mar 25.
  PUBMED    26991063
REFERENCE   2  (bases 1 to 9050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/jacs.5b08980"; journalName: "J Am Chem Soc"; date: 
            "2016-04-6- 6"; volume: "138"; issue: "13"; pages: "4278-81"
COMMENT     Sequence Label: pMaRSC
FEATURES             Location/Qualifiers
     source          1..9050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        17..396
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        397..600
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     551..571
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     645..664
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        645..663
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             692..715
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2780..2791
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(3032..3043)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             3422..3475
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3476..4183
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3621..3639)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3889..3908
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4184..4213
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(4254..4271)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4260..4484
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4530..4958
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4617..4636)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4827..4848
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4967..4987)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4972..5301
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5152..5287
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5214..5233
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5350..6375
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5350..6375
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6505..6626
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6542..6561)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6596..6615
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6675..6691)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6675..6691)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6688..6710)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6699..6715
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6723..6729,6730..6747,6748..6753))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6768..6789
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6906..6923)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7077..7665)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7157..7176)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7836..8627,8628..8696))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8459..8478
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8697..8801)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8876..8895)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      421 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      481 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      541 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      601 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
      661 agggagaccc aagctggcta gcgccactat ggattacaag gatgatgatg ataagatgag
      721 actgttcgtg agcgagggtt ccccggggag cctgcccgtg ctggctgcgg ccgcgagggc
      781 ccggggtcgg gcggagctgc tcatcagcac cgtaggcccc gaagagtgtg tggtaccatt
      841 ccttacccgg cctaaggtcc ctgtcttgca gctggatagt ggcaactacc tcttctctgc
      901 tagtgcaatc tgccgatatt tttttctgtt atgtggctgg gaacaagatg atctcaccaa
      961 ccagtggctg gaatgggagg caacagaact gcagccagtt ctgtctgctg ccctacactg
     1021 tctagtggtt caaggcaaga aaggggaaga tatacttggc ccacttcgga gagtcctgac
     1081 tcacattgat cacagcttga gtcgtcaaaa ctgtcctttc ctggctgggg acacagaatc
     1141 tctagctgac attgttttgt ggggagcact gtatccttta ctgcaagacc cagcttacct
     1201 ccctgaggag ttgggtgccc tgcaaagttg gttccagaca ctgagtaccc aggaaccgtg
     1261 tcagcgagct gcagagacgg tgctaaaaca gcagggtgtc ctggcacttc gtctgtacct
     1321 ccagaaacag ccacagcctc agcccccgcc tcctgagggg agaactgtca gcaacgagct
     1381 ggaggaagag gaactggcta ccttgtctga ggaggacatc gttacagctg ttgccgcgtg
     1441 ggagaagggt ctggaaagcc tgcctccgct aaagctccag cagcatccag tgttgcctgt
     1501 gcctggagag aggaatgttc tcatcaccag tgccggcccc tatgtcaaca atgtccccca
     1561 ccttggaaac atcattggct gtgtgctcag tgctgatgtc tttgcaaggt attgtcgcct
     1621 tcgccagtgg aataccctct atctgtgtgg tacagatgag tatggtactg cgacagagac
     1681 caaggccatg gaggagggcc taaccccacg ggaaatctgt gacaagtacc atgccatcca
     1741 tgctgacatc taccgctggt tcggcatatc gttcgatact ttcgggcgca ctaccactcc
     1801 tcagcagacc aaaatcaccc aggacatctt ccagaggttg ctgacccggg ggtttgtgct
     1861 gcgagatact gtggagcagc ttcggtgtga gcggtgtgca cgtttcctgg ctgaccgctt
     1921 tgtggagggt gtgtgtccct tctgtggcta tgaagaggcc cgaggtgacc agtgtgacag
     1981 gtgtggcaag ctcatcaatg ccattgagct caagaaacca cagtgcaaaa tctgccgctc
     2041 ctgccctgtg gtgaggtcct cacagcacct gtttctagac ttgcctaagt tggaaaagcg
     2101 tctggaggac tggttgggga agacagtgcc tggcagtgac tggacaccca atgccaggtt
     2161 cattatacgt tcctggcttc gagatggcct caagccacga tgcatcacca gagacctcaa
     2221 atggggaacg cctgtgccct tggaaggttt tgaggacaag gtattttacg tctggtttga
     2281 tgctactatt ggctacgtgt ccatcacagc caactacaca gaccaatggg agaaatggtg
     2341 gaagaaccca gaacaagtgg acctttacca gttcatggcc aaagacaatg ttcccttcca
     2401 tggcttggtc tttccgtgtt cagtcctagg agctgaggac aactacaccc tggtcaagca
     2461 catcattgct acagagtacc tgaactatga ggatgggaaa ttctctaaga gccggggcat
     2521 aggagtgttt ggagacatgg ccaaggatac aggaatccct gctgacatct ggcgattcta
     2581 tctgctatac attcggcctg agggccagga cagtgccttc tcctggacag acttgttgat
     2641 taaaaacaat tctgagctgc tcaacaacct gggcaacttc atcaacagag ctggcatgtt
     2701 tgtttctaag ttttttggcg gttgtgtgcc tgagatggcg ctaacccctg atgacagacg
     2761 cctggtggcc catgtctctt gggaactcca gcactatcac cagctgttgg aaaaggttcg
     2821 gatccgggat gccttgcgca gtatcctcac catatctcgc catggcaacc aatacattca
     2881 agtgaatgag ccctggaaac ggattaaagg tggtgagatg gacaggcagc gggcaggcac
     2941 agtgacaggc atggcagtga acatggctgc cttgctgtct gtcatgctgc agccatacat
     3001 gcccacagtc agctctacca tccagaccca gctgcagctc ccacctgcag cctgccgcat
     3061 ccttgccaca agcttcattt gtaccttgcc agcaggccac cgaattggca cagtcagtcc
     3121 tttgttccaa aaactggaaa atgaccagat tgaaaatttg aggcagcgct ttggaggggg
     3181 tcaggctaaa ggctccccca agccagcagc tgtggaggca gttacagcag caggctcgca
     3241 gcacatacaa acgctgacgg atgaggtgac caagcagggc aacgtcgtcc gggaactgaa
     3301 agcacagaag gcagacaaga accaggttgc tgcagaggtg gctaaactct tggatctaaa
     3361 gaaacagttg gctttggctg aggggaaacc cattgaaact cctaaaggca agaagaaaaa
     3421 ggaagggaga ggaagcctcc taacatgcgg tgacgtggag gagaacccag gaccaatggt
     3481 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     3541 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3601 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     3661 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     3721 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     3781 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     3841 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     3901 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     3961 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     4021 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     4081 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     4141 cgagggccgc cactccaccg gcggcatgga cgagctgtac aaggaacaaa aacttatttc
     4201 tgaagaagat ctgtaactcg agtctagagg gcccgtttaa acccgctgat cagcctcgac
     4261 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     4321 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     4381 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     4441 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
     4501 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
     4561 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4621 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4681 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4741 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4801 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4861 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4921 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
     4981 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5041 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5101 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5161 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5221 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
     5281 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
     5341 cagcacgtga tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa
     5401 aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc
     5461 agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc
     5521 tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg
     5581 cttgacattg gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt
     5641 gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag
     5701 gccatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga
     5761 ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc
     5821 catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct
     5881 ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg
     5941 gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg
     6001 agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg
     6061 tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca
     6121 ggatcgccgc ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc
     6181 ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc
     6241 cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg
     6301 accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg
     6361 agggcaaagg aatagcacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
     6421 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     6481 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     6541 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6601 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     6661 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6721 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6781 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6841 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6901 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6961 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     7021 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7081 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7141 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7201 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7261 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7321 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7381 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7441 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7501 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7561 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7621 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7681 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7741 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7801 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7861 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7921 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7981 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     8041 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8101 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8161 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8221 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8281 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8341 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8401 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8461 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8521 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8581 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8641 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8701 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8761 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8821 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     8881 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     8941 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     9001 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
//
