LOCUS       sequence-17571-e         802 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Negative control vector for pMKO.1 puro (Addgene plasmid #8452); 
            Contains shRNA against GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 802)
  AUTHORS   Masutomi K, Possemato R, Wong JM, Currier JL, Tothova Z, Manola JB, 
            Ganesan S, Lansdorp PM, Collins K, Hahn WC
  TITLE     The telomerase reverse transcriptase regulates chromatin state and 
            DNA damage responses.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Jun 7. 102(23):8222-7.
  PUBMED    15928077
REFERENCE   2  (bases 1 to 802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Jun 7. 102(23):8222-7."
COMMENT     Sequence Label: pMKO.1 puro GFP shRNA
FEATURES             Location/Qualifiers
     source          1..802
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..144
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(285..305)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        290..619
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      470..605
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(629..648)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 ggctgttaga gagataatta gaattaattt gactgtaaac acaaagatat tagtacaaaa
       61 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      121 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      181 cttgtggaaa ggacgaaaca ccggctacgt ccaggagcgc accctcgagg gtgcgctcct
      241 ggacgtagcc tttttgaatt cgccagcaca gtggtcgacc ctgtggaatg tgtgtcagtt
      301 agggtgtgga aagtccccag gcttcccagc aggcagaagt atgcaaagca tgcatctcaa
      361 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      421 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      481 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      541 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      601 aggcctaggc ttttgcaaaa agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
      661 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
      721 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
      781 actcttcctc acgcgcgtcg gg
//
