LOCUS       sequence-175724-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  recombinant expression of PTBP1-RRM2 C250S,C251S in e.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Dorn G, Leitner A, Boudet J, Campagne S, von Schroetter C, Moursy A,
            Aebersold R, Allain FH
  TITLE     Structural modeling of protein-RNA complexes using crosslinking of 
            segmentally isotope-labeled RNA and MS/MS.
  JOURNAL   Nat Methods. 2017 Mar 27. doi: 10.1038/nmeth.4235.
  PUBMED    28346450
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Mar 27. doi: 10.1038/nmeth.4235."
COMMENT     Sequence Label: pTYB11-PTBP1-RRM2CCtoSS
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             355..366
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gtctacggac aatatcggta ctcgtgaaac atttcttgct ggtctaattg attctgatgg
       61 ctatgttact gatgagcatg gtattaaagc aacaataaag acaattcata cttctgtcag
      121 agatggtttg gtttcccttg ctcgttcttt aggcttagta gtctcggtta acgcagaacc
      181 tgctaaggtt gacatgaatg tcaccaaaca taaaattagt tatgctattt atatgtctgg
      241 tggagatgtt ttgcttaacg ttctttcgaa gtgtgccggc tctaaaaaat tcaggcctgc
      301 tcccgccgct gcttttgcac gtgagtgccg cggattttat ttcgagttac aagaattgaa
      361 ggaagacgat tattatggga ttactttatc tgatgattct gatcatcagt ttttgcttgg
      421 atcccaggtt gttgtacaga acgccgggca gagccctgtg ctcaggatca tcgtggagaa
      481 cctcttctac cctgtgaccc tggatgtgct gcaccagatt ttctccaagt tcggcacagt
      541 gttgaagatc atcaccttca ccaagaacaa ccagttccag gccctgctgc agtatgcgga
      601 ccccgtgagc gcccagcacg ccaagctgtc gctggacggg cagaacatct acaacgcctc
      661 ctccacgctg cgcatcgact tttccaagct caccagcctc aacgtcaagt acaacaatga
      721 caagagccgt gactacacac gcccagacct gccttccggg gacagccagc cctcgctgga
      781 ccagaccatg gccgcggcct tcggcctttc cgttccgaac gtccacggcg ccctggcccc
      841 cctggccatc ccctcggcgt gactcgagcc cgggtgactg caggaagggg atccggctgc
      901 taacaaagcc cgaaag
//
