LOCUS       sequence-175727-        6686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of human progesterone receptor amino acid 
            residues 1-688 in pCMV5 with 5 restriction sites deleted in 
            polylinker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Liu Q, Su S, Blackwelder AJ, Minges JT, Wilson EM
  TITLE     Gain in transcriptional activity by primate-specific coevolution of 
            melanoma antigen-A11 and its interaction site in androgen receptor.
  JOURNAL   J Biol Chem. 2011 Aug 26;286(34):29951-63. doi: 
            10.1074/jbc.M111.244715. Epub 2011 Jul 5.
  PUBMED    21730049
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M111.244715"; journalName: "J Biol Chem"; date: 
            "2011-08-26- 26"; volume: "286"; issue: "34"; pages: "29951-63"
COMMENT     Sequence Label: p5M-PR-B-1-688
FEATURES             Location/Qualifiers
     source          1..6686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     polyA_signal    2710..3332
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2909..2928)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3356..3376)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3361..3690
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3541..3676
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3728..3747)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3729..3747)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3761..3777)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3761..3777)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3774..3796)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3785..3801
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3809..3815,3816..3833,3834..3839))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3854..3875
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3992..4009)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4163..4751)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4243..4262)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4922..5713,5714..5782))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5545..5564
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5783..5887)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5914..6369
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6001..6020)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6211..6232
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6495..6517
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6509..6526
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6510..6526
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattccac catgactgag ctgaaggcaa agggtccccg ggctccccac gtggcgggcg
      661 gcccgccctc ccccgaggtc ggatccccac tgctgtgtcg cccagccgca ggtccgttcc
      721 cggggagcca gacctcggac accttgcctg aagtttcggc catacctatc tccctggacg
      781 ggctactctt ccctcggccc tgccagggac aggacccctc cgacgaaaag acgcaggacc
      841 agcagtcgct gtcggacgtg gagggcgcat attccagagc tgaagctaca aggggtgctg
      901 gaggcagcag ttctagtccc ccagaaaagg acagcggact gctggacagt gtcttggaca
      961 ctctgttggc gccctcaggt cccgggcaga gccaacccag ccctcccgcc tgcgaggtca
     1021 ccagctcttg gtgcctgttt ggccccgaac ttcccgaaga tccaccggct gcccccgcca
     1081 cccagcgggt gttgtccccg ctcatgagcc ggtccgggtg caaggttgga gacagctccg
     1141 ggacggcagc tgcccataaa gtgctgcccc ggggcctgtc accagcccgg cagctgctgc
     1201 tcccggcctc tgagagccct cactggtccg gggccccagt gaagccgtct ccgcaggccg
     1261 ctgcggtgga ggttgaggag gaggatggct ctgagtccga ggagtctgcg ggtccgcttc
     1321 tgaagggcaa acctcgggct ctgggtggcg cggcggctgg aggaggagcc gcggctgtcc
     1381 cgccgggggc ggcagcagga ggcgtcgccc tggtccccaa ggaagattcc cgcttctcag
     1441 cgcccagggt cgccctggtg gagcaggacg cgccgatggc gcccgggcgc tccccgctgg
     1501 ccaccacggt gatggatttc atccacgtgc ctatcctgcc tctcaatcac gccttattgg
     1561 cagcccgcac tcggcagctg ctggaagacg aaagttacga cggcggggcc ggggctgcca
     1621 gcgcctttgc cccgccgcgg agttcaccct gtgcctcgtc caccccggtc gctgtaggcg
     1681 acttccccga ctgcgcgtac ccgcccgacg ccgagcccaa ggacgacgcg taccctctct
     1741 atagcgactt ccagccgccc gctctaaaga taaaggagga ggaggaaggt gcggaggcct
     1801 ccgcgcgctc cccgcgttcc taccttgtgg ccggtgccaa ccccgcagcc ttcccggatt
     1861 tcccgttggg gccaccgccc ccgctgccgc cgcgagcgac cccatccaga cccggggaag
     1921 cggcggtgac ggccgcaccc gccagtgcct cagtctcgtc tgcgtcctcc tcggggtcga
     1981 ccctggagtg catcctgtac aaagcggagg gcgcgccgcc ccagcagggc ccgttcgcgc
     2041 cgccgccctg caaggcgccg ggcgcgagcg gctgcctgct cccgcgggac ggcctgccct
     2101 ccacctccgc ctctgccgcc gccgccgggg cggcccccgc gctctaccct gcactcggcc
     2161 tcaacgggct cccgcagctc ggctaccagg ccgccgtgct caaggagggc ctgccgcagg
     2221 tctacccgcc ctatctcaac tacctgaggc cggattcaga agccagccag agcccacaat
     2281 acagcttcga gtcattacct cagaagattt gtttaatctg tggggatgaa gcatcaggct
     2341 gtcattatgg tgtccttacc tgtgggagct gtaaggtctt ctttaagagg gcaatggaag
     2401 ggcagcacaa ctacttatgt gctggaagaa atgactgcat cgttgataaa atccgcagaa
     2461 aaaactgccc agcatgtcgc cttagaaagt gctgtcaggc tggcatggtc cttggaggtc
     2521 gaaaatttaa aaagttcaat aaagtcagag ttgtgagagc actggatgct gttgctctcc
     2581 cacagccatt gggcgttcca aatgaaagcc aagccctaag ccagagattc actttttcac
     2641 caggtcaaga catacagttg attccaccac tgatcgatct ggtaccacgc gtatcgataa
     2701 gctgatcccg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     2761 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     2821 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     2881 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     2941 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     3001 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     3061 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     3121 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     3181 gtccttctga ttttaaaata actataccag caggaggacg tccagacaca gcataggcta
     3241 cctggccatg cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc
     3301 gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc ggccagcttg gctgtggaat
     3361 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     3421 atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga
     3481 agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc
     3541 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     3601 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     3661 ggcttttttg gaggcctagg cttttgcaaa aagctcctcg aggaactgaa aaaccagaaa
     3721 gttaattccc tatagtgagt cgtattaaat tcgtaatcat gtcatagctg tttcctgtgt
     3781 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     3841 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     3901 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     3961 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4021 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4081 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4141 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4201 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4261 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4321 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4381 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4441 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4501 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4561 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     4621 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     4681 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4741 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4801 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     4861 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     4921 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     4981 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5041 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5101 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5161 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5221 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5281 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5341 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5401 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5461 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5521 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5581 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     5641 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     5701 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     5761 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     5821 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     5881 ttccgcgcac atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg
     5941 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6001 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6061 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6121 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     6181 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     6241 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     6301 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     6361 ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
     6421 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt
     6481 aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgcca agctgatcta
     6541 tacattgaat caatattggc aattagccat attagtcatt ggttatatag cataaatcaa
     6601 tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg
     6661 ctcatgtcca atatgaccgc catgtt
//
