LOCUS       sequence-17573-e         572 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Masutomi K, Possemato R, Wong JM, Currier JL, Tothova Z, Manola JB, 
            Ganesan S, Lansdorp PM, Collins K, Hahn WC
  TITLE     The telomerase reverse transcriptase regulates chromatin state and 
            DNA damage responses.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Jun 7. 102(23):8222-7.
  PUBMED    15928077
REFERENCE   2  (bases 1 to 572)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Jun 7. 102(23):8222-7."
COMMENT     Sequence Label: pMKO.1 puro hTERT shRNA
FEATURES             Location/Qualifiers
     source          1..572
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        54..302
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     54..74
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     225..244
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(385..405)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 cactccttct ctggcgccgg aattgaagat ctgtcgacaa ggtcgcgttt gaagagggcc
       61 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataatta
      121 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
      181 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
      241 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
      301 ccgtttcatc agcaagtttg gattcaagag atccaaactt gctgatgaaa tttttgaatt
      361 cgccagcaca gtggtcgacc ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      421 gcttcccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      481 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      541 caaccatagt catgcccctg actccgacta tg
//
