LOCUS       sequence-175730-        3928 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of p14-hARF linked to GAL4 DNA binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3928)
  AUTHORS   Minges JT, Grossman G, Zhang P, Kafri T, Wilson EM
  TITLE     Post-translational Down-regulation of Melanoma Antigen-A11 
            (MAGE-A11) by Human p14-ARF Tumor Suppressor.
  JOURNAL   J Biol Chem. 2015 Oct 9;290(41):25174-87. doi: 
            10.1074/jbc.M115.663641. Epub 2015 Sep 1.
  PUBMED    26330556
REFERENCE   2  (bases 1 to 3928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M115.663641"; journalName: "J Biol Chem"; date: 
            "2015-10-9- 9"; volume: "290"; issue: "41"; pages: "25174-87"
COMMENT     Sequence Label: GAL-ARF
FEATURES             Location/Qualifiers
     source          1..3928
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(55..75)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             397..837
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    1552..1686
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1589..1608)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1643..1662
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1749..1768)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2033..2050)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2204..2792)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2284..2303)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2963..3754,3755..3823))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3586..3605
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3824..3928)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacct tctgaggcgg aaagaaccag ctgtggaatg
       61 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      121 tgcatctcaa ttagtcagca aggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      181 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      241 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
      301 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgaag aggctttttt
      361 ggaggagatc taagcttgaa gcaagcctcc tgaaagatga agctactgtc ttctatcgaa
      421 caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc
      481 gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg
      541 ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga acagctattt
      601 ctactgattt ttcctcgaga agaccttgac atgattttga aaatggattc tttacaggat
      661 ataaaagcat tgttaacagg attatttgta caagataatg tgaataaaga tgccgtcaca
      721 gatagattgg cttcagtgga gactgatatg cctctaacat tgagacagca tagaataagt
      781 gcgacatcat catcggaaga gagtagtaac aaaggtcaaa gacagttgac tgtatcgccg
      841 gaattcgtgc gcaggttctt ggtgaccctc cggattcggc gcgcgtgcgg cccgccgcga
      901 gtgagggttt tcgtggttca catcccgcgg ctcacggggg agtgggcagc gccaggggcg
      961 cccgccgctg tggccctcgt gctgatgcta ctgaggagcc agcgtctagg gcagcagccg
     1021 cttcctagaa gaccaggtca tgatgatggg cagcgcccga gtggcggagc tgctgctgct
     1081 ccacggcgcg gagcccaact gcgccgaccc cgccactctc acccgacccg tgcacgacgc
     1141 tgcccgggag ggcttcctgg acacgctggt ggtgctgcac cgggccgggg cgcggctgga
     1201 cgtgcgcgat gcctggggcc gtctgcccgt ggacctggct gaggagctgg gccatcgcga
     1261 tgtcgcacgg tacctgcgcg cggctgcggg gggcaccaga ggcagtaacc atgcccgcat
     1321 agatgccgcg gaaggtccct cagacatccc cgattgaaag aaccagagag gctctgagaa
     1381 acctcgggat ccgtcgacat atgctcgagc ccatcgataa tgcatccgag ctctagataa
     1441 gtaatgatca taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctc
     1501 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     1561 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     1621 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     1681 tggatcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
     1741 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
     1801 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
     1861 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
     1921 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgatatg cggtgtgaaa
     1981 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     2041 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     2101 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     2161 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     2221 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     2281 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     2341 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     2401 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     2461 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     2521 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     2581 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2641 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2701 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2761 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2821 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2881 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2941 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     3001 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     3061 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     3121 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     3181 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     3241 cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
     3301 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     3361 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     3421 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     3481 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     3541 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     3601 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3661 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3721 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3781 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3841 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3901 agaaaaataa acaaataggg gttccgcg
//
