LOCUS       sequence-175733-        7306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Deactivated AsCpf1-SRDX repressor fusion; transcriptional repressor 
            dCpf1 Gateway entry plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7306)
  AUTHORS   Tang X, Lowder LG, Zhang T, Malzahn AA, Zheng X, Voytas DF, Zhong Z,
            Chen Y, Ren Q, Li Q, Kirkland ER, Zhang Y, Qi Y
  TITLE     A CRISPR-Cpf1 system for efficient genome editing and 
            transcriptional repression in plants.
  JOURNAL   Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18.
  PUBMED    28211909
REFERENCE   2  (bases 1 to 7306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Plants."; date: "2017-02-17"; volume: "3:17018. doi"; pages: " 
            10.1038/nplants.2017.18"
COMMENT     Sequence Label: pYPQ223 (dAsCpf1-SRDX)
FEATURES             Location/Qualifiers
     source          1..7306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2985..3005)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(3030..3129)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3145..3162)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3145..3161)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3154..3176)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3225..3311
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3403..3430
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3589..3606)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3760..4348)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3840..3859)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4441..5232)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     5663..5679
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5663..5679
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     5684..5703
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5684..5702
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5720..5843)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(5888..6140)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6301..6348)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gcttggtagt ggatcatgag atccactatc tcatggatga cctggctgag atagccctgc
       61 ttcaggtctt tgatcgtgcc cactacggac caggcctgcc tcgcggcgac gcgttccttc
      121 tcccggttgt cgagcttctt ctggtagtcg aactgctgga tggtattgag cgaccgttgc
      181 tcgagaatct ttccggtgga gtcgatgacg gtgatgtaga tgaggttccg ctccccccgg
      241 gcaatcccga ttatgggtgt ctctgggtgc tccttgagat acgcgttgac ccgctggttg
      301 aacttggacg ggctattcgc ggcctggtag ttcagcgtga tggggacatg gaaaaagaac
      361 ttgtcagagg taaaccgccg atcctttatg atctcgtggc tgacctcctt cgtgatgacg
      421 tttggcagga gcgcgcgggc ctcgtcgctg aggtcgtgtg agaggcggtg gttcacgtaa
      481 tcgtacagct cctgatacag tgtatcaggg attggcgtct tttgatcttt cagcttcttg
      541 ttcagcatct tctcaccgag gcgatgagcc atgcgcttca tcctggactt cggcctgtag
      601 aagagttctg cctggccgtt cagctttatg ctcgtcttcg cgagattttc cgggctgaag
      661 aggcctgtcc agtagagggt gtgcaggttt ggcttcccgt ggtggccctt cgcgaagtcc
      721 ttgttataga tttggaaaag atacaacttg ccggtttcga cggcgtccat gatctccttt
      781 tccgcgatac gctgaaacga gatgtgatac aggagtgggt tgagttcggc gtagtactcc
      841 ccgaggtcct tgtactggga agaggggcgg agggaagaga gatcgatgga ggttgtcttg
      901 gtgtacttgc tgaggaaatc gcgcgtgaag tcgatccact tgcagagcgc ctctctatac
      961 cccttttgat cgcccgtctt cttggcgtag gcggtctgga acttcttggg ttccttttct
     1021 ggattattca gatcgtatat ctccttggtg atctcgagcg gctcgatgaa gttgttggag
     1081 aggaggatgg gggtggtatg ggtctgaaag tgcgccgtca ccgccttaag ctgagtgctg
     1141 cacttcggta tcatctttgc agcgtcagga aaataatcgt agtacatctt gtcgaagccc
     1201 tcggaggtct tctcagttgg ctcgaagctg agcgccttgt acctgccttt ttgtttcggc
     1261 ataattccca aataatacaa cccatttttc acgaaaagaa tggctccgtt gttcttctct
     1321 ttgttaacgt cccacccgct ggccagggtg ggcatttgga agttcagctt gaacttctcc
     1381 acggaatacg gcttcttggt cgcgtaattg cgcgccttat tatagaagct caagctaggt
     1441 tccatctcaa gcttgatccc ggtgaggcgg gcgctaaact ccggatcgac ctcgttggac
     1501 tcgtccacgg cgaaccagtc gagaaggtgg tacagcccca ggaggctgtc cagctgggac
     1561 ttgaggattt ccttttcctc ttgctttttc agggtcgtcg gcagcggctg gtccagggcc
     1621 gcgtgggcgt gagagaggat ctccgaggtc ttctgcttga aagcctcgga cagctccttg
     1681 cccgcggcgg agatgatctc ctgcaggtta atgtcctcgt gcttgaggct cctttgcact
     1741 ttctctttcg ccgacttcgt gatcttgccg gtgagctcgc tgatgcgccg ctcgtagagg
     1801 gcgttgcgga gggtgtccca gtggtcgcac agggcgctgg agatggtttc cagcttctta
     1861 tgggagatga agatgtgggt gagatcgatg ctattgagct cattgaacag cgcctcggcg
     1921 gtttcgagca cattctcgtt gcggaggagg gtcttgtact tgcagaagga ctggatgacc
     1981 tcctcgtcgc tcttgaactc ctcgaggatg aaggacaacg tgttgcggtc ggagaggatc
     2041 tgcttaaaca gggggataaa cctatgtggg agtgaggcaa tgatgtgcgc ggtttcgtcg
     2101 ttcttctgga tggcgagatt gagcacctcg ttcaggccct tgatcttctc ggtgccggcc
     2161 tcgcgggaga tgccgccgag gagctggttg tagaggtcga tctgggtctg cgtcagcagc
     2221 tgattgtaga aggggaagga gaacacctct tcgatcgagg tggacacgaa gatcccgatg
     2281 gccttcttca cgttctcgaa gtgctcgcgg agggacggca cggccgtgat gaggcgggtg
     2341 aagatgtggc agttctcctt gaacttcggg aagttgtctt gcacgatcct atgcgggatg
     2401 gcggtgctaa tgtcctcggc gctgaacacg tttttgcggt tctcgtaaaa gccgctaaag
     2461 tatgtcgtaa acttgtcgaa cgaccggagg agggcgttct catgctctgt ggtcgtgacg
     2521 gtgcccagtt gcttcagcac tttgccattg aacagctcgg ccttaaacag ccccttatag
     2581 atttcggcgt ggcgcttgtt gatggcgtcg gtgagattat ctgtgcgccc gatgaagtag
     2641 tcgtggatag cgttgcgata cgtagcctgt tcttcgatga gcgcgttgcg tgtctcctcc
     2701 gtcttctcct tgcggtagga gtcgatggcg gccgagaggt tctcccagtc gagctgcacg
     2761 agctgcaggc actggtccgc gtaggtcttg tagatccggt cgatgatcgg cttgagctcc
     2821 ttgtagtggt cgttccgcgc tttgtcctcc tcgataaagc cctgctcctg gatgtgcttg
     2881 agggtcttgc cctgcggaat cagctcgaac cgcagcgtct tgctgacctg gtagaggttg
     2941 gtgaacccct cgaactgcgt agccgcaggc accccgtgaa taccaacctt ccgcttcttc
     3001 ttaggagcca tggtgaaggg cgaattcgga gcctgctttt ttgtacaaag ttggcattat
     3061 aaaaaagcat tgctcatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat
     3121 cattatttgg ggcccgagct taagactggc cgtcgtttta caacgtcgtg actgggaaaa
     3181 catccatgca agcgttaacg cgagagtagg gaactgccag gcatcaaata aaacgaaagg
     3241 ctcagtcgga agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctcctga
     3301 gtaggacaaa tccgccggga gcggatttga acgttgtgaa gcaacggccc ggagggtggc
     3361 gggcaggacg cccgccataa actgccaggc atcaaactaa gcagaaggcc atcctgacgg
     3421 atggcctttt tgcgtttcta caaactcttc ctggctcgcg gtacgcgtat taattgcgtt
     3481 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     3541 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     3601 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     3661 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     3721 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     3781 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     3841 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     3901 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     3961 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4021 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4081 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4141 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4201 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     4261 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     4321 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     4381 cgctcagtgg aacgacgcgt aactcacgtt aagggatttt ggtcatgggt ggctcgaggg
     4441 ttatttgccg actaccttgg tgatctcgcc tttcacgtag tggacaaatt cttccaactg
     4501 atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga taagcctgtc tagcttcaag
     4561 tatgacgggc tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt
     4621 cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt
     4681 tcgctcatcg ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc
     4741 ctcaaataga tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa
     4801 ggcaacgcta tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc
     4861 tggctcgaag atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg
     4921 cttagctgga taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc
     4981 gcggagaatc tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc
     5041 tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg
     5101 tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc
     5161 gagatggcgc tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac
     5221 cgcttccctc ataatgttta actttgtttt agggcgactg ccctgctgcg taacatcgtt
     5281 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     5341 aggcatagac tgtaccccaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc
     5401 gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt
     5461 gcattacagc ttacgaaccg aacaggctta tgtccactgg gttcgtgcct tcatccgttt
     5521 ccacggtgtg cgtcacccgg caaccttggg tagcagcgaa gtcgaggcat ttctgtcctg
     5581 gctggtccag aggaggcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
     5641 ttgagacacg ggccagagct gccaggaaac agctatgacc atgtaatacg actcactata
     5701 ggggatatca gctggatggc atagtgactg gatatgttgt gttttacagt attatgtagt
     5761 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
     5821 ttcaactttt gtatacaaag ttgcgaattc gccctttccg gaagctgtcg aggggggatc
     5881 aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta gtttgcgcgc
     5941 tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat cataaaaacc
     6001 catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac gtaattcaac
     6061 agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa gaaactttat
     6121 tgccaaatgt ttgaacgatc ggacgtccta agcgaatccg agtctaagct caagatcgag
     6181 gtcaagagca aatccgagtc taagttcaag gtcgagatcg agagcaaatc caagtctgag
     6241 ttcgaggtca agatcaaggt cgactgagcc agaacccgac cccgatccag agccgtctcc
     6301 cttctttttc ttagcctgtc cggccttttt ggtggcagca ggacgcttat tgcggagctc
     6361 ttggatatag gcaagccaat cctggttgct gatgccgttc tgcagcttaa ggtccttgct
     6421 ctccttgaga tggttcaaca acagttgtcc tttcaatgct atgtggtatg cgccgttagc
     6481 atcggcatcc atcggccact caggattctg aaatctagaa tcgaagcaca cgccattgag
     6541 atcacgcaca ggggagttga tgtaatcctc cccagtggca gcatttgaat ttctcatttg
     6601 cagcacggat ctgatgagcg ctaccatcgt gtcaatagcg tggctatcat cgttctcgag
     6661 cagtttgggg aggatatttg acccgtcgcg gaaaacgatg cctttctcct ccaggagggc
     6721 gatgagttcg tttgcggggt acaggtcacg gtaacgcccg gtgaatctat ggttctcaat
     6781 tacagggaca atcctcttgc ccgcgatgaa aggcgtgcct ttggcgtcga attgggtttc
     6841 atttttctca aaaacgatat cccaagcggg catgaagcca gggagtcccc gctggaagga
     6901 gaggttccga ttcatcttga aatgcaggat gaagtctccg gtttttacat catagtgcag
     6961 gaagtcgaaa ccctccagga aatgcttacg cgactcatgg ttcttgatgg tcttccagac
     7021 aaacgggtcc acgaatccgg tcaagggatc aatctttgag gtatatgggg ccgggacata
     7081 gaaaaggaag ccgctctggg tgcccatctt cgcaaatgac gtaaactggt cggtcagctg
     7141 atacggattg aggacccccc cgaccttctc cgccggatag tccttgagca ccaggcaatt
     7201 gagcttgtcg ataagcattt tctcgaactg ttggtacacc gccttctcgg cgatgcctgt
     7261 gcgcttggat ttgaatccga aattgagatt ctcgaggacc acgacg
//
