LOCUS       sequence-175734-        8294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of full-length human progesterone receptor-A in
            pCMV5 with 3 restriction sites deleted in polylinker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Su S, Blackwelder AJ, Grossman G, Minges JT, Yuan L, Young SL, 
            Wilson EM
  TITLE     Primate-specific melanoma antigen-A11 regulates isoform-specific 
            human progesterone receptor-B transactivation.
  JOURNAL   J Biol Chem. 2012 Oct 5;287(41):34809-24. doi: 
            10.1074/jbc.M112.372797. Epub 2012 Aug 13.
  PUBMED    22891251
REFERENCE   2  (bases 1 to 8294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.372797"; journalName: "J Biol Chem"; date: 
            "2012-10-5- 5"; volume: "287"; issue: "41"; pages: "34809-24"
COMMENT     Sequence Label: p5M-PR-A
FEATURES             Location/Qualifiers
     source          1..8294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     RBS             4307..4318
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     polyA_signal    4390..5012
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(4589..4608)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(5036..5056)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5080..5290
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        5102..5298
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5149..5284
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5336..5355)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5337..5355)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5369..5385)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5369..5385)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5382..5404)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5393..5409
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5417..5423,5424..5441,5442..5447))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5462..5483
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5600..5617)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5771..6359)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5851..5870)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6530..7321,7322..7390))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7153..7172
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7391..7495)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7522..7977
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7609..7628)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7819..7840
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8103..8125
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8117..8134
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8118..8134
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agaattcaga tccccactgc tgtgtcgccc agccgcaggt ccgttcccgg ggagccagac
      661 ctcggacacc ttgcctgaag tttcggccat acctatctcc ctggacgggc tactcttccc
      721 tcggccctgc cagggacagg acccctccga cgaaaagacg caggaccagc agtcgctgtc
      781 ggacgtggag ggcgcatatt ccagagctga agctacaagg ggtgctggag gcagcagttc
      841 tagtccccca gaaaaggaca gcggactgct ggacagtgtc ttggacactc tgttggcgcc
      901 ctcaggtccc gggcagagcc aacccagccc tcccgcctgc gaggtcacca gctcttggtg
      961 cctgtttggc cccgaacttc ccgaagatcc accggctgcc cccgccaccc agcgggtgtt
     1021 gtccccgctc atgagccggt ccgggtgcaa ggttggagac agctccggga cggcagctgc
     1081 ccataaagtg ctgccccggg gcctgtcacc agcccggcag ctgctgctcc cggcctctga
     1141 gagccctcac tggtccgggg ccccagtgaa gccgtctccg caggccgctg cggtggaggt
     1201 tgaggaggag gatggctctg agtccgagga gtctgcgggt ccgcttctga agggcaaacc
     1261 tcgggctctg ggtggcgcgg cggctggagg aggagccgcg gctgtcccgc cgggggcggc
     1321 agcaggaggc gtcgccctgg tccccaagga agattcccgc ttctcagcgc ccagggtcgc
     1381 cctggtggag caggacgcgc cgatggcgcc cgggcgctcc ccgctggcca ccacggtgat
     1441 ggatttcatc cacgtgccta tcctgcctct caatcacgcc ttattggcag cccgcactcg
     1501 gcagctgctg gaagacgaaa gttacgacgg cggggccggg gctgccagcg cctttgcccc
     1561 gccgcggagt tcaccctgtg cctcgtccac cccggtcgct gtaggcgact tccccgactg
     1621 cgcgtacccg cccgacgccg agcccaagga cgacgcgtac cctctctata gcgacttcca
     1681 gccgcccgct ctaaagataa aggaggagga ggaaggtgcg gaggcctccg cgcgctcccc
     1741 gcgttcctac cttgtggccg gtgccaaccc cgcagccttc ccggatttcc cgttggggcc
     1801 accgcccccg ctgccgccgc gagcgacccc atccagaccc ggggaagcgg cggtgacggc
     1861 cgcacccgcc agtgcctcag tctcgtctgc gtcctcctcg gggtcgaccc tggagtgcat
     1921 cctgtacaaa gcggagggcg cgccgcccca gcagggcccg ttcgcgccgc cgccctgcaa
     1981 ggcgccgggc gcgagcggct gcctgctccc gcgggacggc ctgccctcca cctccgcctc
     2041 tgccgccgcc gccggggcgg cccccgcgct ctaccctgca ctcggcctca acgggctccc
     2101 gcagctcggc taccaggccg ccgtgctcaa ggagggcctg ccgcaggtct acccgcccta
     2161 tctcaactac ctgaggccgg attcagaagc cagccagagc ccacaataca gcttcgagtc
     2221 attacctcag aagatttgtt taatctgtgg ggatgaagca tcaggctgtc attatggtgt
     2281 ccttacctgt gggagctgta aggtcttctt taagagggca atggaagggc agcacaacta
     2341 cttatgtgct ggaagaaatg actgcatcgt tgataaaatc cgcagaaaaa actgcccagc
     2401 atgtcgcctt agaaagtgct gtcaggctgg catggtcctt ggaggtcgaa aatttaaaaa
     2461 gttcaataaa gtcagagttg tgagagcact ggatgctgtt gctctcccac agccattggg
     2521 cgttccaaat gaaagccaag ccctaagcca gagattcact ttttcaccag gtcaagacat
     2581 acagttgatt ccaccactga tcaacctgtt aatgagcatt gaaccagatg tgatctatgc
     2641 aggacatgac aacacaaaac ctgacacctc cagttctttg ctgacaagtc ttaatcaact
     2701 aggcgagagg caacttcttt cagtagtcaa gtggtctaaa tcattgccag gttttcgaaa
     2761 cttacatatt gatgaccaga taactctcat tcagtattct tggatgagct taatggtgtt
     2821 tggtctagga tggagatcct acaaacatgt cagtgggcag atgctgtatt ttgcacctga
     2881 tctaatacta aatgaacagc ggatgaaaga atcatcattc tattcattat gccttaccat
     2941 gtggcagatc ccacaggagt ttgtcaagct tcaagttagc caagaagagt tcctctgtat
     3001 gaaagtattg ttacttctta atacaattcc tttggaaggg ctacgaagtc aaacccagtt
     3061 tgaggagatg aggtcaagct acattagaga gctcatcaag gcaattggtt tgaggcaaaa
     3121 aggagttgtg tcgagctcac agcgtttcta tcaacttaca aaacttcttg ataacttgca
     3181 tgatcttgtc aaacagcttc atctgtactg cttgaataca tttatccagt cccgggcact
     3241 gagtgttgaa tttccagaaa tgatgtctga agttattgct gcacaattac ccaagatatt
     3301 ggcagggatg gtgaaacccc ttctctttca taaaaagtga atgtcatctt tttcttttaa
     3361 agaattaaat tttgtggcat gtctttttgt tttggtcagg attatgaggt cttgagtttt
     3421 tataatgttc ttctgaaagc cttacattta taacatcata gtgtgtaaat ttaaaagaaa
     3481 aattgtgagg ttctaattat tttcttttat aaagtataat tagaatgttt aactgttttg
     3541 tttacccata ttttcttgaa gaatttacaa gattgaaaaa gtactaaaat tgttaaagta
     3601 aactatatct tatccatatt atttcatacc atgtaggtga ggatttttaa cttttgcatc
     3661 taacaaatca tcgacttaag agaaaaaatc ttacatgtaa taacacaaag ctattatatg
     3721 ttatttctag gtaactccct ttgtgtcaat tatatttcca aaaatgaacc tttaaaatgg
     3781 tatgcaaaat tttgtctata tatatttgtg tgaggaggaa attcataact ttcctcagat
     3841 tttcaaaagt atttttaatg caaaaaatgt agaaagagtt taaaaccact aaaatagatt
     3901 gatgttcttc aaactaggca aaacaactca tatgttaaga ccattttcca gattggaaac
     3961 acaaatctct taggaagtta ataagtagat tcatatcatt atacaaatag tattgtgggt
     4021 tttgtaggtt tttaaaataa ccttttttgg ggagagaatt gtcctctaat gaggtattgc
     4081 gagtggacat aagaaatcag aagattatgg cctaactgta ctccttacca actgtggcat
     4141 gctgaaagtt agtcactctt actgattctc aattctctca cctttgaaag tagtaaaata
     4201 tctttcctgc caattgctcc tttgggtcag agcttattaa catcttttca aatcaaagga
     4261 aagaagaaag ggagaggagg aggagggaga tatcaattca catacctttc tcctctttat
     4321 cctccactat catgaattca gatcatccat cacactggcg gccgctcgag catgcatcta
     4381 gaggatcccg ggtggcatcc ctgtgacccc tccccagtgc ctctcctggc cctggaagtt
     4441 gccactccag tgcccaccag ccttgtccta ataaaattaa gttgcatcat tttgtctgac
     4501 taggtgtcct tctataatat tatggggtgg aggggggtgg tatggagcaa ggggcaagtt
     4561 gggaagacaa cctgtagggc ctgcggggtc tattgggaac caagctggag tgcagtggca
     4621 caatcttggc tcactgcaat ctccgcctcc tgggttcaag cgattctcct gcctcagcct
     4681 cccgagttgt tgggattcca ggcatgcatg accaggctca gctaattttt gtttttttgg
     4741 tagagacggg gtttcaccat attggccagg ctggtctcca actcctaatc tcaggtgatc
     4801 tacccacctt ggcctcccaa attgctggga ttacaggcgt gaaccactgc tcccttccct
     4861 gtccttctga ttttaaaata actataccag caggaggacg tccagacaca gcataggcta
     4921 cctggccatg cccaaccggt gggacatttg agttgcttgc ttggcactgt cctctcatgc
     4981 gttgggtcca ctcagtagat gcctgttgaa ttgggtacgc ggccagcttg gctgtggaat
     5041 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5101 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     5161 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5221 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5281 ggcctaggct tttgcaaaaa gctcctcgag gaactgaaaa accagaaagt taattcccta
     5341 tagtgagtcg tattaaattc gtaatcatgt catagctgtt tcctgtgtga aattgttatc
     5401 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     5461 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     5521 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     5581 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     5641 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     5701 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     5761 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     5821 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     5881 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     5941 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     6001 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     6061 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     6121 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     6181 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     6241 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     6301 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     6361 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     6421 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     6481 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     6541 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     6601 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     6661 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     6721 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     6781 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     6841 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     6901 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     6961 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     7021 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     7081 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     7141 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     7201 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     7261 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     7321 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     7381 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     7441 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     7501 ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     7561 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     7621 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     7681 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     7741 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     7801 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     7861 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     7921 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttcca
     7981 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     8041 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     8101 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctgatctata cattgaatca
     8161 atattggcaa ttagccatat tagtcattgg ttatatagca taaatcaata ttggctattg
     8221 gccattgcat acgttgtatc tatatcataa tatgtacatt tatattggct catgtccaat
     8281 atgaccgcca tgtt
//
