LOCUS       sequence-175736-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant expression of PTBP1-RRM12 C250S,C251S in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Dorn G, Leitner A, Boudet J, Campagne S, von Schroetter C, Moursy A,
            Aebersold R, Allain FH
  TITLE     Structural modeling of protein-RNA complexes using crosslinking of 
            segmentally isotope-labeled RNA and MS/MS.
  JOURNAL   Nat Methods. 2017 Mar 27. doi: 10.1038/nmeth.4235.
  PUBMED    28346450
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Mar 27. doi: 10.1038/nmeth.4235."
COMMENT     Sequence Label: pET28a-PTBP1-RRM12CCtoSS
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             60..77
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             924..941
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc
       61 tggtgccgcg cggcagccat atgaacggaa atgacagcaa gaagttcaaa ggtgacagcc
      121 gaagtgcagg cgtcccctct agagtgatcc acatccggaa gctccccatc gacgtcacgg
      181 agggggaagt catctccctg gggctgccct ttgggaaggt caccaacctc ctgatgctga
      241 aggggaaaaa ccaggccttc atcgagatga acacggagga ggctgccaac accatggtga
      301 actactacac ctcggtgacc cctgtgctgc gcggccagcc catctacatc cagttctcta
      361 accacaagga gctgaagacc gacagctctc ccaaccaggc gcgggcccag gcggccctgc
      421 aggcggtgaa ctcggtccag tcggggaacc tggccttggc tgcctcggcg gcggccgtgg
      481 acgcagggat ggcgatggcc gggcagagcc ctgtgctcag gatcatcgtg gagaacctct
      541 tctaccctgt gaccctggat gtgctgcacc agattttctc caagttcggc acagtgttga
      601 agatcatcac cttcaccaag aacaaccagt tccaggccct gctgcagtat gcggaccccg
      661 tgagcgccca gcacgccaag ctgtcgctgg acgggcagaa catctacaac gccagcagca
      721 cgctgcgcat cgacttttcc aagctcacca gcctcaacgt caagtacaac aatgacaaga
      781 gccgtgacta cacacgccca gacctgcctt ccggggacag ccagccctcg ctggaccaga
      841 ccatggccgc ggccttcggc ctttccgttc cgaacgtcca cggcgccctg gcccccctgg
      901 ccatcccctc ggcgtgactc gagcaccacc accaccacca ctga
//
