LOCUS       sequence-17575-e         611 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Chen W, Possemato R, Campbell KT, Plattner CA, Pallas DC, Hahn WC
  TITLE     Identification of specific PP2A complexes involved in human cell 
            transformation.
  JOURNAL   Cancer Cell. 2004 Feb . 5(2):127-36.
  PUBMED    14998489
REFERENCE   2  (bases 1 to 611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2004 Feb . 5(2):127-36."
COMMENT     Sequence Label: pMKO.1 GFP B56 alpha shRNA 6-3
FEATURES             Location/Qualifiers
     source          1..611
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..299
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     51..71
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     222..241
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(384..404)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tccttctcta ggcgccggaa ttgaagatct gtcgacaggt cgggcaggaa gagggcctat
       61 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa
      121 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
      181 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
      241 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
      301 gaacagaata gtgcttacaa cactcgagtg ttgtaagcac tattctgttt ttttgaattc
      361 gccagcacag tggtcgaccc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
      421 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
      481 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      541 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      601 ttctccgccc c
//
