LOCUS       sequence-17576-e         664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Chen W, Possemato R, Campbell KT, Plattner CA, Pallas DC, Hahn WC
  TITLE     Identification of specific PP2A complexes involved in human cell 
            transformation.
  JOURNAL   Cancer Cell. 2004 Feb . 5(2):127-36.
  PUBMED    14998489
REFERENCE   2  (bases 1 to 664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2004 Feb . 5(2):127-36."
COMMENT     Sequence Label: pMKO.1 GFP B56 gamma3 shRNA 14-1
FEATURES             Location/Qualifiers
     source          1..664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        50..298
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     50..70
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     221..240
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(383..403)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tccttctcag gcgccggaat tgaagatctg tcgacaggtc gggcaggaag agggcctatt
       61 tcccatgatt ccttcatatt tgcatatacg atacaaggct gttagagaga taattagaat
      121 taatttgact gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt
      181 tcttgggtag tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt
      241 aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg
      301 aagatgaacc aacgttagaa gctcgagctt ctaacgttgg ttcatctttt tttgaattcg
      361 ccagcacagt ggtcgaccct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
      421 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
      481 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      541 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
      601 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
      661 tctg
//
