LOCUS       sequence-17580-e         584 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Hahn WC, Dessain SK, Brooks MW, King JE, Elenbaas B, Sabatini DM, 
            DeCaprio JA, Weinberg RA
  TITLE     Enumeration of the simian virus 40 early region elements necessary 
            for human cell transformation.
  JOURNAL   Mol Cell Biol 2002 Apr;22(7):2111-23.
  PUBMED    11884599
REFERENCE   2  (bases 1 to 584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol"; date: "2002-04"; volume: "22(7)"; pages: "2111-23"
COMMENT     Sequence Label: pMIG small T
FEATURES             Location/Qualifiers
     source          1..584
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      26..35
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             32..556
                     /codon_start=1
                     /product="SV40 (simian virus 40) small t antigen"
                     /label=small t antigen
                     /translation="MDKVLNREESLQLMDLLGLERSAWGNIPLMRKAYLKKCKEFHPDK
                     GGDEEKMKKMNTLYKKMEDGVKYAHQPDFGGFWDATEVFASSLNPGVDAMYCKQWPECA
                     KKMSANCICLLCLLRMKHENRKLYRKDPLVWVDCYCFDCFRMWFGLDLCEGTLLLWCDI
                     IGQTTYRDLKL"
ORIGIN
        1 ttctctaggc gccggaatta gatccgccac catggataaa gttttaaaca gagaggaatc
       61 tttgcagcta atggaccttc taggtcttga aaggagtgcc tgggggaata ttcctctgat
      121 gagaaaggca tatttaaaaa aatgcaagga gtttcatcct gataaaggag gagatgaaga
      181 aaaaatgaag aaaatgaata ctctgtacaa gaaaatggaa gatggagtaa aatatgctca
      241 tcaacctgac tttggaggct tctgggatgc aactgaggta tttgcttctt ccttaaatcc
      301 tggtgttgat gcaatgtact gcaaacaatg gcctgagtgt gcaaagaaaa tgtctgctaa
      361 ctgcatatgc ttgctgtgct tactgaggat gaagcatgaa aatagaaaat tatacaggaa
      421 agatccactt gtgtgggttg attgctactg cttcgattgc tttagaatgt ggtttggact
      481 tgatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
      541 agatttaaag ctctaagaat tccgcccccc cccccccccc cccc
//
