LOCUS       sequence-17587-e         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Meister G, Landthaler M, Patkaniowska A, Dorsett Y, Teng G, Tuschl T
  TITLE     Human Argonaute2 mediates RNA cleavage targeted by miRNAs and 
            siRNAs.
  JOURNAL   Mol Cell. 2004 Jul 23. 15(2):185-97.
  PUBMED    15260970
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2004 Jul 23. 15(2):185-97."
COMMENT     Sequence Label: pIRESneo-FLAG/HA Ago1
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..52
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             53..79
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cgactggatc gattcgccgg ccgccatgga ctacaaggac gacgatgaca agtaccctta
       61 tgacgtgccc gattacgcta gcggccgcat ggaagcggga ccctcgggag cagctgcggg
      121 cgcttacctg ccccccctgc agcaggtgtt ccaggcacct cgccggcctg gcattggcac
      181 tgtggggaaa ccaatcaagc tcctggccaa ttactttgag gtggacatcc ctaagatcga
      241 cgtgtaccac tacgaggtgg acatcaagcc ggataagtgt ccccgtagag tcaaccggga
      301 agtggtggaa tacatggtcc agcatttcaa gcctcagatc tttggtgatc gcaagcctgt
      361 gtatgatgga aagaagaaca tttacactgt cacagcactg cccattggca acgaacgggt
      421 cgactttgag gtgacaatcc ctggggaagg gaaggatcga atctttaagg tctccatcaa
      481 gtggctagcc attgtgagct ggcgaatgct gcatgaggcc ctggtcagcg gccagatccc
      541 tgttcccttg gagtctgtgc aagccctgga tgtggccatg aggcacctgg catccatgag
      601 gtacacccct gtgggccgct ccttcttctc accgcctgag ggctactacc acccgctggg
      661 gggtgggcgc gaggtctggt tcggctttca ccagtctgtg cgccctgcca tgtggaagat
      721 gatgctcaac attgatgtct cagccactgc ctt
//
