LOCUS       sequence-176056-        7069 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Deactivated LbCpf1-SRDX repressor fusion; transcriptional repressor 
            dCpf1 Gateway entry plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7069)
  AUTHORS   Tang X, Lowder LG, Zhang T, Malzahn AA, Zheng X, Voytas DF, Zhong Z,
            Chen Y, Ren Q, Li Q, Kirkland ER, Zhang Y, Qi Y
  TITLE     A CRISPR-Cpf1 system for efficient genome editing and 
            transcriptional repression in plants.
  JOURNAL   Nat Plants. 2017 Feb 17;3:17018. doi: 10.1038/nplants.2017.18.
  PUBMED    28211909
REFERENCE   2  (bases 1 to 7069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Plants."; date: "2017-02-17"; volume: "3:17018. doi"; pages: " 
            10.1038/nplants.2017.18"
COMMENT     Sequence Label: pYPQ233 (dLbCpf1-SRDX)
FEATURES             Location/Qualifiers
     source          1..7069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1638..1658)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(1683..1782)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1798..1815)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1798..1814)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1807..1829)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      1878..1964
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2056..2083
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(2242..2259)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2413..3001)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2493..2512)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3094..3885)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     4316..4332
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4316..4332
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4337..4356
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4337..4355
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(4373..4496)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      complement(4541..4793)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4954..5001)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 tccttgtcct tgtcccagcc gcccatgaac tgggggttct ggaagtagag tttgaacttg
       61 tccttgctgt acggcttttg tgtcacgtaa tttctgattg cgtcgtagat gtggtctacc
      121 ttaaggagta tatcgtacgc gaggacgaag tctccgtaga aggactcatc tctgttagtc
      181 tccttcccct ccccgaaaaa agccttaata tagttctcga aactcttcac gctgtcgagc
      241 agatccttca taatggcaac aacagcgtcg ttcttcttca gggacttctc caaaacgaag
      301 tcggcgtcga acagtttttc acttgatcca tagactttat atatctcgtc gaccttttgg
      361 atgattatct ccttcaactt ctcgacgacg ctaagatcgg cgtcagcata ctcctgcaac
      421 tgttccaggc tgaaacttcc gatctttttg aagcttttcc tgcgatcatc ctcgtacttc
      481 tccgttacga cagccttctt cttcaggtga atatcgtcat actccgcgtt ccatttgtct
      541 ctgatcacgt tccattcgcc gaagatatct ttacttatcg ttgatatggc cgggccgttt
      601 ttcacaaata tgccggcgct agagtattcg tcaaaattct taaacaactt ttccagtttt
      661 tttatagaag agaaaatctc cgaattctta ttgagagtat tcctgaacac ctccaggact
      721 tcctcatccg aagtgtagcc ctccccatag aacgagagtg actcgcggtc tgaaagaacc
      781 tgtttgtaaa gaggcttgaa ctttggcagc ttctgtttcg tcttttggtt gtaaaggttg
      841 atatactcgt tcaatccctt aatcttttca ccggactcag tcacgaaccc accgatgatc
      901 gcattataca cgtcgatacc ctcttgagtg agcacgaaat taaaaaactc accttcgaag
      961 aagtcctcaa cgtcataatc ggaattgagg atcttctcct taatctcctg aacttcgtgc
     1021 ttgtcaaaaa ttgcgtcaac cttctcaaag atatccatgt tggaaatgta cctagtgaga
     1081 ttctcattga tacacctgaa cgcaatagaa gtggactttg cctcctcaga gaacatattt
     1141 tcccgattat caaagaagcc tgtgaatgct gttgtgaatc cgttgaacga attgaccagc
     1201 gcaatctcgt ccttgtcatc gaggaactct ggcaaaattg tctcaattat atccttcttg
     1261 aacagacttt tgtatccttc gttgccttta aaggctttcg cgatctcttt cctcagattg
     1321 atctccaaat tttcaagttc cttattctcc ttttctgtcc ttgttttctt tctgaagagg
     1381 gagatgtagt tgttcagatt cttcaatttg attgagtgca agacatcatt gataaaagag
     1441 aggtagtatc tgtccaacaa cttcttcacc cctttgtagt cttccgcgcg cttttcatct
     1501 tccaccaaca gccgcttatt gtctatattc tcttgagtct tgccgactgg aatcgcttta
     1561 aaccgcagtg ttttgctcaa cgaataacag ttggtgaatt tctcgagctt tgaagccgca
     1621 ggcaccccgt gaataccaac cttccgcttc ttcttaggag ccatggtgaa gggcgaattc
     1681 ggagcctgct tttttgtaca aagttggcat tataaaaaag cattgctcat caatttgttg
     1741 caacgaacag gtcactatca gtcaaaataa aatcattatt tggggcccga gcttaagact
     1801 ggccgtcgtt ttacaacgtc gtgactggga aaacatccat gcaagcgtta acgcgagagt
     1861 agggaactgc caggcatcaa ataaaacgaa aggctcagtc ggaagactgg gcctttcgtt
     1921 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
     1981 tgaacgttgt gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
     2041 ggcatcaaac taagcagaag gccatcctga cggatggcct ttttgcgttt ctacaaactc
     2101 ttcctggctc gcggtacgcg tattaattgc gttgcgctca ctgcccgctt tccagtcggg
     2161 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     2221 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     2281 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     2341 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     2401 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     2461 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     2521 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     2581 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     2641 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     2701 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     2761 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     2821 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     2881 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     2941 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     3001 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgacg cgtaactcac
     3061 gttaagggat tttggtcatg ggtggctcga gggttatttg ccgactacct tggtgatctc
     3121 gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc
     3181 ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag
     3241 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
     3301 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
     3361 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
     3421 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
     3481 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
     3541 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
     3601 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
     3661 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
     3721 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
     3781 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
     3841 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcataatgt ttaactttgt
     3901 tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca aacatcgacc
     3961 cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc caaaaaaaca
     4021 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
     4081 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa ccgaacaggc
     4141 ttatgtccac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt
     4201 gggtagcagc gaagtcgagg catttctgtc ctggctggtc cagaggaggc atgatgatat
     4261 atttttatct tgtgcaatgt aacatcagag attttgagac acgggccaga gctgccagga
     4321 aacagctatg accatgtaat acgactcact ataggggata tcagctggat ggcatagtga
     4381 ctggatatgt tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa
     4441 tatattgata tttatatcat tttacgtttc tcgttcaact tttgtataca aagttgcgaa
     4501 ttcgcccttt ccggaagctg tcgagggggg atcaattccc gatctagtaa catagatgac
     4561 accgcgcgcg ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa
     4621 atgtataatt gcgggactct aatcataaaa acccatctca taaataacgt catgcattac
     4681 atgttaatta ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga
     4741 ccggcaacag gattcaatct taagaaactt tattgccaaa tgtttgaacg atcggacgtc
     4801 ctaagcgaat ccgagtctaa gctcaagatc gaggtcaaga gcaaatccga gtctaagttc
     4861 aaggtcgaga tcgagagcaa atccaagtct gagttcgagg tcaagatcaa ggtcgactga
     4921 gccagaaccc gaccccgatc cagagccgtc tcccttcttt ttcttagcct gtccggcctt
     4981 tttggtggca gcaggacgct tatgctttac ggatgtctga gcatactcca gccactcttt
     5041 gttgctgata gcgattttga ccttgtccaa tttttcgtct tcggcctttt tgaactggcc
     5101 gatcgcccac aggactttcc tggcgatgtt ataggcaccg ttggcgtctg cattttttgg
     5161 cagaatcgca ttctcttgcg cttcatagtt cctgctatcg taaaagatgc catcagaatt
     5221 tttaaccggg gagatcaaga aatccacgtc ggttctgcct gtaattgaat ttctcatctg
     5281 gagcatcagg ctcatcaaag ccatgaagga tgagtagaaa gccttgtcag attgttcgca
     5341 aagcagtgct ctgatatcac cctgttggta gttaatgcca tacttattaa agagctcctt
     5401 gtacgcgcta gtcaagcaaa cttcctccca atcaaagacg ttattcttct tagggttgcg
     5461 aaaaatcctg attctgttgc cgtacgaata gagtttccat tttttaatgt aatccgcatc
     5521 ggtccgcgaa aagtttttgt agtcgagagc aaactcgaaa agatcttcct ctgggacgta
     5581 cataatacga tcaaaactag atatgaactt tttcgaatcg gcaatggacg tgtatttcgt
     5641 cttcaacagg ttgacaaaac cagttgacgg gtcgatctta gacgtaagcc acgccggtat
     5701 ataaaatatg aacccattct gagtactcat cgatttgaag gactcgaatt tgtttgttat
     5761 ttggtaacct ttgagtgcgc cgccggtggc acatgggttc gatttcttat cgaccatgta
     5821 gttgagctta tctataagca tcttctcgaa cttttgatag acctgcttct ccactttaac
     5881 tctagaattc ttaaagcccg agttgaggtc ctcaagagcg atcactgcgt catacttctc
     5941 aaccagctcg catatcttat gcacaacttg agagatataa cctgctttga gttccttgat
     6001 gttctcgata cttgtccaat tttgccgcgc ctcaaatctc tccttctcct tcttgtccag
     6061 caacgaatgg tagtcggtct tgatacggat gccgttgaaa ttgttaatta tttcgttaag
     6121 ggagtactgt tcgactatgt tccccttccc gtccaccacc acaatataga ggaggttcct
     6181 ctcgccccga gcaatcccaa tgacgtaagg attgtcgtca tgcttcagga gtacacgcac
     6241 ctctgtgttt atcttgaaaa tattctttgg gcacttgttg atcgcaatcg ggatgtggag
     6301 ctcgtactga tcctcggaaa acctcttatc cttgtacacg tcgtagctca atgttgtagt
     6361 ctttttagga ttgtccgggt tcttattcgc aattggacta ttcgcagggt ggactacaag
     6421 ctcctccttc ttaaggctag cgcgccgcat gaagagttca gcccctcctg acaacctgat
     6481 ttggccgtgg ttattctcgt cgaagagcag cttgaaatac attgtgtgga gattaggtgt
     6541 cccgtggctc ttatcgctaa agtctttatt ataaatctgg aacatgtaga gcttaccctc
     6601 ttccacgagc ttgtccactt ccttcttgct ggcggactcg aatgacacct tgtagccctg
     6661 ctcttccacc tctctataaa atccggctat atccttgtac ttctcggttt ccgaaaagtt
     6721 gaagtcatag gcgttagacc actttgggta ccggctaatt gagtctttga agaagtcgat
     6781 cagcttgtga cagtcgttaa gattgaacat gtcgcccttc ttgaacgtac cgttcttata
     6841 aatcttctgg atgtcctcgc ttgggttata gtaggccatc catttcttgc tgaagaaaac
     6901 ctttgggagc atcttgttcg gacccggaag cagcttgtag ttgatcttct cataattgcc
     6961 attgacatcg tctttgtcaa tcttctggag acacttggcg tacttcttgt ccataatcgc
     7021 caagtagtat tttgaaccat acctcaggat cgtagccctg taatccgtt
//
