LOCUS       sequence-176058-        1757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombinant expression of PTBP1 C250S,C251S in E.coli .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Dorn G, Leitner A, Boudet J, Campagne S, von Schroetter C, Moursy A,
            Aebersold R, Allain FH
  TITLE     Structural modeling of protein-RNA complexes using crosslinking of 
            segmentally isotope-labeled RNA and MS/MS.
  JOURNAL   Nat Methods. 2017 Mar 27. doi: 10.1038/nmeth.4235.
  PUBMED    28346450
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2017 Mar 27. doi: 10.1038/nmeth.4235."
COMMENT     Sequence Label: pET28a-PTBP1CCtoSS
FEATURES             Location/Qualifiers
     source          1..1757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..50
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             60..77
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             1723..1740
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac agcagcggcc
       61 tggtgccgcg cggcagccat atggacggca ttgtcccaga tatagccgtt ggtacaaagc
      121 ggggatctga cgagcttttc tctacttgtg tcactaacgg accgtttatc atgagcagca
      181 actcggcttc tgcagcaaac ggaaatgaca gcaagaagtt caaaggtgac agccgaagtg
      241 caggcgtccc ctctagagtg atccacatcc ggaagctccc catcgacgtc acggaggggg
      301 aagtcatctc cctggggctg ccctttggga aggtcaccaa cctcctgatg ctgaagggga
      361 aaaaccaggc cttcatcgag atgaacacgg aggaggctgc caacaccatg gtgaactact
      421 acacctcggt gacccctgtg ctgcgcggcc agcccatcta catccagttc tctaaccaca
      481 aggagctgaa gaccgacagc tctcccaacc aggcgcgggc ccaggcggcc ctgcaggcgg
      541 tgaactcggt ccagtcgggg aacctggcct tggctgcctc ggcggcggcc gtggacgcag
      601 ggatggcgat ggccgggcag agccctgtgc tcaggatcat cgtggagaac ctcttctacc
      661 ctgtgaccct ggatgtgctg caccagattt tctccaagtt cggcacagtg ttgaagatca
      721 tcaccttcac caagaacaac cagttccagg ccctgctgca gtatgcggac cccgtgagcg
      781 cccagcacgc caagctgtcg ctggacgggc agaacatcta caacgcctcc tccacgctgc
      841 gcatcgactt ttccaagctc accagcctca acgtcaagta caacaatgac aagagccgtg
      901 actacacacg cccagacctg ccttccgggg acagccagcc ctcgctggac cagaccatgg
      961 ccgcggcctt cggcctttcc gttccgaacg tccacggcgc cctggccccc ctggccatcc
     1021 cctcggcggc ggcggcagct gcggcggcag gtcggatcgc catcccgggc ctggcggggg
     1081 caggaaattc tgtattgctg gtcagcaacc tcaacccaga gagagtcaca ccccaaagcc
     1141 tctttattct tttcggcgtc tacggtgacg tgcagcgcgt gaagatcctg ttcaataaga
     1201 aggagaacgc cctagtgcag atggcggacg gcaaccaggc ccagctggcc atgagccacc
     1261 tgaacgggca caagctgcac gggaagccga tccgcatcac gctctcgaag caccagaacg
     1321 tgcagctgcc ccgcgagggc caggaggacc agggcctgac caaggactac ggcaactcac
     1381 ccctgcaccg cttcaagaag ccgggctcca agaacttcca gaacatattc ccgccctcgg
     1441 ccactctgca cctctccaac atcccgccct cagtctccga ggaggatctc aaggtcctgt
     1501 tttccagcaa tgggggcgtc gtcaaaggat tcaagttctt ccagaaggac cgcaagatgg
     1561 cactgatcca gatgggctcc gtggaggagg cggtccaggc cctcattgac ctgcacaacc
     1621 acgacctcgg ggagaaccac cacctgcggg tctccttctc caagtccacc atctagggat
     1681 ccgaattcga gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac
     1741 tgagatccgg ctgctaa
//
