LOCUS       sequence-176066-        5088 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of full-length human MAGE-A11 with N-terminal 
            VP16 activation domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5088)
  AUTHORS   Bai S, He B, Wilson EM
  TITLE     Melanoma antigen gene protein MAGE-11 regulates androgen receptor 
            function by modulating the interdomain interaction.
  JOURNAL   Mol Cell Biol. 2005 Feb;25(4):1238-57.
  PUBMED    15684378
REFERENCE   2  (bases 1 to 5088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-02"; volume: "25(4)"; pages: "1238-57"
COMMENT     Sequence Label: VP-MAGE-2-429
FEATURES             Location/Qualifiers
     source          1..5088
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    170..304
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(207..226)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     261..280
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(314..333)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(315..333)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(420..437)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(591..1179)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(671..690)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1350..2141,2142..2210))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1973..1992
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2211..2315)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2383..2401
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2439..2461)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2561..2580
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2661..2679
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2661..2678
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2689..2709)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     regulatory      3017..3026
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3029..3049
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3050..3283
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 aaaaaaaaaa aaaaaaaaaa aaaaaaaact cgacgcgtct gcagaagctt ctagataagt
       61 aatgatcata atcagccata tcacatctgt agaggtttta cttgctttaa aaaacctccc
      121 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
      181 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
      241 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
      301 gatctgccgg tctccctata gtgagtcgta ttaatttcga taagccaggt taacctgcat
      361 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
      421 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
      481 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      541 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      601 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      661 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      721 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      781 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      841 tgtgtgcatg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      901 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      961 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     1021 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     1081 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     1141 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     1201 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     1261 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     1321 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     1381 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     1441 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     1501 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     1561 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     1621 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     1681 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     1741 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     1801 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     1861 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     1921 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     1981 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     2041 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     2101 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     2161 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     2221 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2281 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     2341 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
     2401 ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg
     2461 gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg
     2521 tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta
     2581 ctgagagtgc accatatgga catattgtcg ttagaacgcg gctacaatta atacataacc
     2641 ttatgtatca tacacatacg atttaggtga cactatagaa ctcgatgtgg aatgtgtgtc
     2701 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     2761 tcaattagtc agcaaggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     2821 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     2881 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     2941 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaagaggctt ttttggagga
     3001 gatctaagct agcgccgcca ccatgggccc taaaaagaag cgtaaagtcg cccccccgac
     3061 cgatgtcagc ctgggggacg agctccactt agacggcgag gacgtggcga tggcgcatgc
     3121 cgacgcgcta gacgatttcg atctggacat gttgggggac ggggattccc cgggtccggg
     3181 atttaccccc cacgactccg ccccctacgg cgctctggat atggccgact tcgagtttga
     3241 gcagatgttt accgatgccc ttggaattga cgagtacggt ggggaattcg agactcagtt
     3301 ccgcagaggg ggtctggggt gcagccctgc cagcatcaag aggaagaaga agagggagga
     3361 ctcaggagac tttggactcc aggtgagcac tatgttctca gaggacgact tccagtcaac
     3421 agaaagagcc ccatatggtc cacaactaca gtggtcccag gatctgccaa gagtccaggt
     3481 ttttagagaa caggccaacc tggaggacag gagtcccagg agaacccaga ggatcactgg
     3541 aggagaacaa gtgctgtggg gccccatcac ccagatattt cccacagttc ggcctgctga
     3601 cctaaccaga gtcatcatgc ctcttgagca aagaagtcag cactgcaagc ctgaggaagg
     3661 ccttcaggcc caagaagaag acctgggcct ggtgggtgca caggctctcc aagctgagga
     3721 gcaggaggct gccttcttct cctctactct gaatgtgggc actctagagg agttgcctgc
     3781 tgctgagtca ccaagtcctc cccagagtcc tcaggaagag tccttctctc ccactgccat
     3841 ggatgccatc tttgggagcc tatctgatga gggctctggc agccaagaaa aggaggggcc
     3901 aagtacctcg cctgacctga tagaccctga gtccttttcc caagatatac tacatgacaa
     3961 gataattgat ttggttcatt tattgctccg caagtatcga gtcaaggggc tgatcacaaa
     4021 ggcagaaatg ctggggagtg tcatcaaaaa ttatgaggac tactttcctg agatatttag
     4081 ggaagcctct gtatgcatgc aactgctctt tggcattgat gtgaaggaag tggaccccac
     4141 tagccactcc tatgtccttg tcacctccct caacctctct tatgatggca tacagtgtaa
     4201 tgagcagagc atgcccaagt ctggcctcct gataatagtc ctgggtgtaa tcttcatgga
     4261 ggggaactgc atccctgaag aggttatgtg ggaagtcctg agcattatgg gggtgtatgc
     4321 tggaagggag cacttcctct ttggggagcc caagaggctc cttacccaaa attgggtgca
     4381 ggaaaagtac ctggtgtacc ggcaggtgcc cggcactgat cctgcatgct atgagttcct
     4441 gtggggtcca agggcccacg ctgagaccag caagatgaaa gttcttgagt acatagccaa
     4501 tgccaatggg agggatccca cttcttaccc atccctgtat gaagatgctt tgagagagga
     4561 gggagaggga gtctgagcat gagatgcaac cagggccagc gggcagggaa atgggccaat
     4621 gcatgcttca gggccacacc cagcagtttc cctgtcctgt gtgaaatcag gcccattctt
     4681 ccctctgtgt ttgatgagag aagtcagtgt tctcagtagt agaaggcaca gtgaatggaa
     4741 gggaacacat tgtatactgc ctttaggttt ctcttccatc gggtgacttg gagatttctt
     4801 tttgtttccc tttggtaatt ttcaaatatt gttcctgtaa taaaagtttt agttagcttc
     4861 aacatctaag tgtatggatg atactgacca cacatgttgt tttgcttatc catttcaagt
     4921 gcaagtgttt gccattttgt aaaacatttt gggaaatctt ccatcttgct gtgatttgca
     4981 ataggtattt tcttggagaa tgtaagaact taacaataaa gctgaactgg tgttgtgaaa
     5041 cagagaaata aaaggagaag gtcattaaaa aaaaaaaaaa aaaaaaaa
//
