LOCUS       sequence-176068-        5157 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of full-length human MAGE-A11 with N-terminal 
            GAL4 DNA binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Bai S, He B, Wilson EM
  TITLE     Melanoma antigen gene protein MAGE-11 regulates androgen receptor 
            function by modulating the interdomain interaction.
  JOURNAL   Mol Cell Biol. 2005 Feb;25(4):1238-57.
  PUBMED    15684378
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2005-02"; volume: "25(4)"; pages: "1238-57"
COMMENT     Sequence Label: GAL-MAGE-2-429
FEATURES             Location/Qualifiers
     source          1..5157
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    156..290
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(193..212)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     247..266
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(353..372)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(637..654)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(808..1396)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(888..907)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1567..2358,2359..2427))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2190..2209
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2428..2532)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2587..2607)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             2929..3369
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 acatatgctc gagcccatcg ataatgcatc cgagctctag ataagtaatg atcataatca
       61 gccatatcac atctgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
      121 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
      181 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
      241 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatcctctac
      301 gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg cgcctatatc
      361 gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag cgcttgtttc
      421 ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat ctccttgcat
      481 gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg ctgcttccta
      541 atgcaggagt cgcataaggg agagcgtcga tatgcggtgt gaaataccgc acagatgcgt
      601 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
      661 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
      721 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
      781 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      841 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      901 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      961 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     1021 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     1081 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     1141 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     1201 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     1261 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     1321 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     1381 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     1441 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1501 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1561 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1621 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1681 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     1741 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1801 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1861 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1921 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1981 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     2041 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     2101 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     2161 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa
     2221 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     2281 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     2341 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     2401 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     2461 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2521 aggggttccg cgcacatttc cccgaaaagt gccacctgac cttctgaggc ggaaagaacc
     2581 agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     2641 gtatgcaaag catgcatctc aattagtcag caaggaaagt ccccaggctc cccagcaggc
     2701 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     2761 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     2821 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     2881 agaggctttt ttggaggaga tctaagcttg aagcaagcct cctgaaagat gaagctactg
     2941 tcttctatcg aacaagcatg cgatatttgc cgacttaaaa agctcaagtg ctccaaagaa
     3001 aaaccgaagt gcgccaagtg tctgaagaac aactgggagt gtcgctactc tcccaaaacc
     3061 aaaaggtctc cgctgactag ggcacatctg acagaagtgg aatcaaggct agaaagactg
     3121 gaacagctat ttctactgat ttttcctcga gaagaccttg acatgatttt gaaaatggat
     3181 tctttacagg atataaaagc attgttaaca ggattatttg tacaagataa tgtgaataaa
     3241 gatgccgtca cagatagatt ggcttcagtg gagactgata tgcctctaac attgagacag
     3301 catagaataa gtgcgacatc atcatcggaa gagagtagta acaaaggtca aagacagttg
     3361 actgtatcgc cggaattcga gactcagttc cgcagagggg gtctggggtg cagccctgcc
     3421 agcatcaaga ggaagaagaa gagggaggac tcaggagact ttggactcca ggtgagcact
     3481 atgttctcag aggacgactt ccagtcaaca gaaagagccc catatggtcc acaactacag
     3541 tggtcccagg atctgccaag agtccaggtt tttagagaac aggccaacct ggaggacagg
     3601 agtcccagga gaacccagag gatcactgga ggagaacaag tgctgtgggg ccccatcacc
     3661 cagatatttc ccacagttcg gcctgctgac ctaaccagag tcatcatgcc tcttgagcaa
     3721 agaagtcagc actgcaagcc tgaggaaggc cttcaggccc aagaagaaga cctgggcctg
     3781 gtgggtgcac aggctctcca agctgaggag caggaggctg ccttcttctc ctctactctg
     3841 aatgtgggca ctctagagga gttgcctgct gctgagtcac caagtcctcc ccagagtcct
     3901 caggaagagt ccttctctcc cactgccatg gatgccatct ttgggagcct atctgatgag
     3961 ggctctggca gccaagaaaa ggaggggcca agtacctcgc ctgacctgat agaccctgag
     4021 tccttttccc aagatatact acatgacaag ataattgatt tggttcattt attgctccgc
     4081 aagtatcgag tcaaggggct gatcacaaag gcagaaatgc tggggagtgt catcaaaaat
     4141 tatgaggact actttcctga gatatttagg gaagcctctg tatgcatgca actgctcttt
     4201 ggcattgatg tgaaggaagt ggaccccact agccactcct atgtccttgt cacctccctc
     4261 aacctctctt atgatggcat acagtgtaat gagcagagca tgcccaagtc tggcctcctg
     4321 ataatagtcc tgggtgtaat cttcatggag gggaactgca tccctgaaga ggttatgtgg
     4381 gaagtcctga gcattatggg ggtgtatgct ggaagggagc acttcctctt tggggagccc
     4441 aagaggctcc ttacccaaaa ttgggtgcag gaaaagtacc tggtgtaccg gcaggtgccc
     4501 ggcactgatc ctgcatgcta tgagttcctg tggggtccaa gggcccacgc tgagaccagc
     4561 aagatgaaag ttcttgagta catagccaat gccaatggga gggatcccac ttcttaccca
     4621 tccctgtatg aagatgcttt gagagaggag ggagagggag tctgagcatg agatgcaacc
     4681 agggccagcg ggcagggaaa tgggccaatg catgcttcag ggccacaccc agcagtttcc
     4741 ctgtcctgtg tgaaatcagg cccattcttc cctctgtgtt tgatgagaga agtcagtgtt
     4801 ctcagtagta gaaggcacag tgaatggaag ggaacacatt gtatactgcc tttaggtttc
     4861 tcttccatcg ggtgacttgg agatttcttt ttgtttccct ttggtaattt tcaaatattg
     4921 ttcctgtaat aaaagtttta gttagcttca acatctaagt gtatggatga tactgaccac
     4981 acatgttgtt ttgcttatcc atttcaagtg caagtgtttg ccattttgta aaacattttg
     5041 ggaaatcttc catcttgctg tgatttgcaa taggtatttt cttggagaat gtaagaactt
     5101 aacaataaag ctgaactggt gttgtgaaac agagaaataa aaggagaagg tcattaa
//
