LOCUS       sequence-176069-        6145 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human MAGE-A11 amino acid residues 112-429 
            with N-terminal FLAG tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6145)
  AUTHORS   Su S, Chen X, Geng J, Minges JT, Grossman G, Wilson EM
  TITLE     Melanoma antigen-A11 regulates substrate-specificity of 
            Skp2-mediated protein degradation.
  JOURNAL   Mol Cell Endocrinol. 2017 Jan 5;439:1-9. doi: 
            10.1016/j.mce.2016.10.006. Epub 2016 Oct 6.
  PUBMED    27720894
REFERENCE   2  (bases 1 to 6145)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Endocrinol."; date: "2017-01-5"; volume: "439:1-9. doi"; pages: " 
            10.1016/j.mce.2016.10.006. Epub 2016 Oct 6"
COMMENT     Sequence Label: FLAG-MAGE-112-429
FEATURES             Location/Qualifiers
     source          1..6145
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    61..683
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(260..279)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(707..727)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        712..1041
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      892..1027
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1079..1098)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1080..1098)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1112..1128)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1112..1128)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1125..1147)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1136..1152
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1160..1166,1167..1184,1185..1190))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1205..1226
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1343..1360)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1514..2102)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1594..1613)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2273..3064,3065..3133))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2896..2915
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3134..3238)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      3265..3720
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3352..3371)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3562..3583
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3846..3868
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3860..3877
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3861..3877
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        4038..4417
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4418..4621
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4572..4592
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4655..4678
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 acatatgctc gagcccatcg atagatctga tatcggtacc agtcgactct agaggatccc
       61 gggtggcatc cctgtgaccc ctccccagtg cctctcctgg ccctggaagt tgccactcca
      121 gtgcccacca gccttgtcct aataaaatta agttgcatca ttttgtctga ctaggtgtcc
      181 ttctataata ttatggggtg gaggggggtg gtatggagca aggggcaagt tgggaagaca
      241 acctgtaggg cctgcggggt ctattgggaa ccaagctgga gtgcagtggc acaatcttgg
      301 ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc tgcctcagcc tcccgagttg
      361 ttgggattcc aggcatgcat gaccaggctc agctaatttt tgtttttttg gtagagacgg
      421 ggtttcacca tattggccag gctggtctcc aactcctaat ctcaggtgat ctacccacct
      481 tggcctccca aattgctggg attacaggcg tgaaccactg ctcccttccc tgtccttctg
      541 attttaaaat aactatacca gcaggaggac gtccagacac agcataggct acctggccat
      601 gcccaaccgg tgggacattt gagttgcttg cttggcactg tcctctcatg cgttgggtcc
      661 actcagtaga tgcctgttga attgggtacg cggccagctt ggctgtggaa tgtgtgtcag
      721 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
      781 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      841 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      901 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
      961 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     1021 ggaggcctag gcttttgcaa aaagctcctc gaggaactga aaaaccagaa agttaattcc
     1081 ctatagtgag tcgtattaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt
     1141 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1201 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1261 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     1321 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     1381 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     1441 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     1501 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     1561 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1621 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1681 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1741 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1801 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1861 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1921 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1981 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     2041 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     2101 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     2161 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     2221 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     2281 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     2341 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     2401 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     2461 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     2521 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     2581 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     2641 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     2701 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     2761 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     2821 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     2881 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     2941 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     3001 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     3061 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     3121 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     3181 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     3241 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     3301 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     3361 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     3421 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     3481 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     3541 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     3601 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     3661 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     3721 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     3781 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     3841 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa
     3901 tcaatattgg caattagcca tattagtcat tggttatata gcataaatca atattggcta
     3961 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
     4021 aatatgaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
     4081 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     4141 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     4201 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     4261 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
     4321 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
     4381 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
     4441 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     4501 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
     4561 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     4621 tcgtttagtg aaccgtcaga attgatctac catggactac aaagacgatg acgacaagct
     4681 tgcggccgac gaattccctc ttgagcaaag aagtcagcac tgcaagcctg aggaaggcct
     4741 tcaggcccaa gaagaagacc tgggcctggt gggtgcacag gctctccaag ctgaggagca
     4801 ggaggctgcc ttcttctcct ctactctgaa tgtgggcact ctagaggagt tgcctgctgc
     4861 tgagtcacca agtcctcccc agagtcctca ggaagagtcc ttctctccca ctgccatgga
     4921 tgccatcttt gggagcctat ctgatgaggg ctctggcagc caagaaaagg aggggccaag
     4981 tacctcgcct gacctgatag accctgagtc cttttcccaa gatatactac atgacaagat
     5041 aattgatttg gttcatttat tgctccgcaa gtatcgagtc aaggggctga tcacaaaggc
     5101 agaaatgctg gggagtgtca tcaaaaatta tgaggactac tttcctgaga tatttaggga
     5161 agcctctgta tgcatgcaac tgctctttgg cattgatgtg aaggaagtgg accccactag
     5221 ccactcctat gtccttgtca cctccctcaa cctctcttat gatggcatac agtgtaatga
     5281 gcagagcatg cccaagtctg gcctcctgat aatagtcctg ggtgtaatct tcatggaggg
     5341 gaactgcatc cctgaagagg ttatgtggga agtcctgagc attatggggg tgtatgctgg
     5401 aagggagcac ttcctctttg gggagcccaa gaggctcctt acccaaaatt gggtgcagga
     5461 aaagtacctg gtgtaccggc aggtgcccgg cactgatcct gcatgctatg agttcctgtg
     5521 gggtccaagg gcccacgctg agaccagcaa gatgaaagtt cttgagtaca tagccaatgc
     5581 caatgggagg gatcccactt cttacccatc cctgtatgaa gatgctttga gagaggaggg
     5641 agagggagtc tgagcatgag atgcaaccag ggccagcggg cagggaaatg ggccaatgca
     5701 tgcttcaggg ccacacccag cagtttccct gtcctgtgtg aaatcaggcc cattcttccc
     5761 tctgtgtttg atgagagaag tcagtgttct cagtagtaga aggcacagtg aatggaaggg
     5821 aacacattgt atactgcctt taggtttctc ttccatcggg tgacttggag atttcttttt
     5881 gtttcccttt ggtaattttc aaatattgtt cctgtaataa aagttttagt tagcttcaac
     5941 atctaagtgt atggatgata ctgaccacac atgttgtttt gcttatccat ttcaagtgca
     6001 agtgtttgcc attttgtaaa acattttggg aaatcttcca tcttgctgtg atttgcaata
     6061 ggtattttct tggagaatgt aagaacttaa caataaagct gaactggtgt tgtgaaacag
     6121 agaaataaaa ggagaaggtc attaa
//
