LOCUS       sequence-176070-        6741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of full-length human MAGE-A11 with N-terminal 
            GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6741)
  AUTHORS   Askew EB, Bai S, Parris AB, Minges JT, Wilson EM
  TITLE     Androgen receptor regulation by histone methyltransferase Suppressor
            of variegation 3-9 homolog 2 and Melanoma antigen-A11.
  JOURNAL   Mol Cell Endocrinol. 2017 Mar 5;443:42-51. doi: 
            10.1016/j.mce.2016.12.028. Epub 2016 Dec 29.
  PUBMED    28042025
REFERENCE   2  (bases 1 to 6741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Endocrinol."; date: "2017-03-5"; volume: "443:42-51. doi"; pages: " 
            10.1016/j.mce.2016.12.028. Epub 2016 Dec 29"
COMMENT     Sequence Label: GST-MAGE-2-429
FEATURES             Location/Qualifiers
     source          1..6741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(74..96)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(241..259)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        327..431
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(432..500,501..1292)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(650..669)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1463..2051
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1952..1971
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2205..2222
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        2295..2372
                     /gene="lacI"
                     /label=lacI promoter
     CDS             2373..3455
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(2392..2411)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        join(3504..3509,3510..3527,3528..3534)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3542..3558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3566..3582
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3566..3582
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3578..4105
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
                     DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
                     TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGISLSTARCTNASGVR
                     QPSEAVVWLCRS"
     primer_bind     complement(3598..3615)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3598..3614)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3607..3629)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        join(4207..4212,4213..4228,4229..4235)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    4243..4259
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4282..4935
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     4893..4915
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             4942..4959
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gacatatgct cgagcggccg catcgtgact gactgacgat ctgcctcgcg cgtttcggtg
       61 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
      121 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
      181 gcgcagccat gacccagtca cgtagcgata gcggagtgta taattcttga agacgaaagg
      241 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
      301 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
      361 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      421 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      481 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      541 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      601 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      661 cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      721 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      781 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      841 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      901 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      961 acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     1021 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     1081 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     1141 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     1201 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     1261 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1321 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1381 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1441 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1501 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1561 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1621 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1681 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1741 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1801 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1861 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1921 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1981 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2041 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2101 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     2161 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     2221 aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     2281 accgcataaa ttccgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc
     2341 gcccggaaga gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg
     2401 cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg
     2461 tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca
     2521 accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca
     2581 gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac
     2641 tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg
     2701 cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg
     2761 accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg
     2821 tctctgacca gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg
     2881 gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa
     2941 gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa
     3001 ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca
     3061 tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg
     3121 cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg
     3181 tagtgggata cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca
     3241 aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg
     3301 gccaggcggt gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc
     3361 tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
     3421 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtaagttag
     3481 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
     3541 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cggattcact
     3601 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     3661 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     3721 ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt gcctggtttc cggcaccaga
     3781 agcggtgccg gaaagctggc tggagtgcga tcttcctgag gccgatactg tcgtcgtccc
     3841 ctcaaactgg cagatgcacg gttacgatgc gcccatctac accaacgtga cctatcccat
     3901 tacggtcaat ccgccgtttg ttcccacgga gaatccgacg ggttgttact cgctcacatt
     3961 taatgttgat gaaagctggc tacaggaagg ccagacgcga attatttttg atggcgttgg
     4021 aattagctta tcgactgcac ggtgcaccaa tgcttctggc gtcaggcagc catcggaagc
     4081 tgtggtatgg ctgtgcaggt cgtaaatcac tgcataattc gtgtcgctca aggcgcactc
     4141 ccgttctgga taatgttttt tgcgccgaca tcataacggt tctggcaaat attctgaaat
     4201 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
     4261 ttcacacagg aaacagtatt catgtcccct atactaggtt attggaaaat taagggcctt
     4321 gtgcaaccca ctcgacttct tttggaatat cttgaagaaa aatatgaaga gcatttgtat
     4381 gagcgcgatg aaggtgataa atggcgaaac aaaaagtttg aattgggttt ggagtttccc
     4441 aatcttcctt attatattga tggtgatgtt aaattaacac agtctatggc catcatacgt
     4501 tatatagctg acaagcacaa catgttgggt ggttgtccaa aagagcgtgc agagatttca
     4561 atgcttgaag gagcggtttt ggatattaga tacggtgttt cgagaattgc atatagtaaa
     4621 gactttgaaa ctctcaaagt tgattttctt agcaagctac ctgaaatgct gaaaatgttc
     4681 gaagatcgtt tatgtcataa aacatattta aatggtgatc atgtaaccca tcctgacttc
     4741 atgttgtatg acgctcttga tgttgtttta tacatggacc caatgtgcct ggatgcgttc
     4801 ccaaaattag tttgttttaa aaaacgtatt gaagctatcc cacaaattga taagtacttg
     4861 aaatccagca agtatatagc atggcctttg cagggctggc aagccacgtt tggtggtggc
     4921 gaccatcctc caaaatcgga tctggttccg cgtggatccc cggaattcga gactcagttc
     4981 cgcagagggg gtctggggtg cagccctgcc agcatcaaga ggaagaagaa gagggaggac
     5041 tcaggagact ttggactcca ggtgagcact atgttctcag aggacgactt ccagtcaaca
     5101 gaaagagccc catatggtcc acaactacag tggtcccagg atctgccaag agtccaggtt
     5161 tttagagaac aggccaacct ggaggacagg agtcccagga gaacccagag gatcactgga
     5221 ggagaacaag tgctgtgggg ccccatcacc cagatatttc ccacagttcg gcctgctgac
     5281 ctaaccagag tcatcatgcc tcttgagcaa agaagtcagc actgcaagcc tgaggaaggc
     5341 cttcaggccc aagaagaaga cctgggcctg gtgggtgcac aggctctcca agctgaggag
     5401 caggaggctg ccttcttctc ctctactctg aatgtgggca ctctagagga gttgcctgct
     5461 gctgagtcac caagtcctcc ccagagtcct caggaagagt ccttctctcc cactgccatg
     5521 gatgccatct ttgggagcct atctgatgag ggctctggca gccaagaaaa ggaggggcca
     5581 agtacctcgc ctgacctgat agaccctgag tccttttccc aagatatact acatgacaag
     5641 ataattgatt tggttcattt attgctccgc aagtatcgag tcaaggggct gatcacaaag
     5701 gcagaaatgc tggggagtgt catcaaaaat tatgaggact actttcctga gatatttagg
     5761 gaagcctctg tatgcatgca actgctcttt ggcattgatg tgaaggaagt ggaccccact
     5821 agccactcct atgtccttgt cacctccctc aacctctctt atgatggcat acagtgtaat
     5881 gagcagagca tgcccaagtc tggcctcctg ataatagtcc tgggtgtaat cttcatggag
     5941 gggaactgca tccctgaaga ggttatgtgg gaagtcctga gcattatggg ggtgtatgct
     6001 ggaagggagc acttcctctt tggggagccc aagaggctcc ttacccaaaa ttgggtgcag
     6061 gaaaagtacc tggtgtaccg gcaggtgccc ggcactgatc ctgcatgcta tgagttcctg
     6121 tggggtccaa gggcccacgc tgagaccagc aagatgaaag ttcttgagta catagccaat
     6181 gccaatggga gggatcccac ttcttaccca tccctgtatg aagatgcttt gagagaggag
     6241 ggagagggag tctgagcatg agatgcaacc agggccagcg ggcagggaaa tgggccaatg
     6301 catgcttcag ggccacaccc agcagtttcc ctgtcctgtg tgaaatcagg cccattcttc
     6361 cctctgtgtt tgatgagaga agtcagtgtt ctcagtagta gaaggcacag tgaatggaag
     6421 ggaacacatt gtatactgcc tttaggtttc tcttccatcg ggtgacttgg agatttcttt
     6481 ttgtttccct ttggtaattt tcaaatattg ttcctgtaat aaaagtttta gttagcttca
     6541 acatctaagt gtatggatga tactgaccac acatgttgtt ttgcttatcc atttcaagtg
     6601 caagtgtttg ccattttgta aaacattttg ggaaatcttc catcttgctg tgatttgcaa
     6661 taggtatttt cttggagaat gtaagaactt aacaataaag ctgaactggt gttgtgaaac
     6721 agagaaataa aaggagaagg t
//
