LOCUS       sequence-176071-        5901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of N-terminal HA-tagged full-length human 
            MAGE-A11 amino acid residues 2-429.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5901)
  AUTHORS   Askew EB, Bai S, Hnat AT, Minges JT, Wilson EM
  TITLE     Melanoma antigen gene protein-A11 (MAGE-11) F-box links the androgen
            receptor NH2-terminal transactivation domain to p160 coactivators.
  JOURNAL   J Biol Chem. 2009 Dec 11;284(50):34793-808. doi: 
            10.1074/jbc.M109.065979. Epub 2009 Oct 14.
  PUBMED    19828458
REFERENCE   2  (bases 1 to 5901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.065979"; journalName: "J Biol Chem"; date: 
            "2009-12-11- 11"; volume: "284"; issue: "50"; pages: "34793-808"
COMMENT     Sequence Label: pSG5HA-MAGE-2-429
FEATURES             Location/Qualifiers
     source          1..5901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    40..161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(77..96)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     131..150
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(195..212)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(366..954)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(446..465)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1125..1916,1917..1985))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1748..1767
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1986..2090)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2158..2176
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2214..2236)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2336..2355
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2422..2877)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2559..2580)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2771..2790
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3003..3025
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3017..3034
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3018..3034
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3071..3091)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3179..3389
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3201..3397
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3248..3383
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          3428..4000
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     4008..4027
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     4055..4074
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4055..4073
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      4084..4093
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4096..4122
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctcgacatat gctcgaggat ccagatctta ttaaagcaga acttgtttat tgcagcttat
       61 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
      121 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gtcgactcta
      181 gactcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
      241 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
      301 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
      361 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
      421 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
      481 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
      541 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
      601 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
      661 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
      721 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
      781 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
      841 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
      901 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
      961 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1021 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1081 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1141 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1201 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1261 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     1321 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     1381 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     1441 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     1501 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     1561 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     1621 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     1681 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     1741 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     1801 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     1861 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     1921 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     1981 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2041 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2101 cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat
     2161 aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     2221 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     2281 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     2341 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     2401 aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     2461 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     2521 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     2581 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     2641 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     2701 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     2761 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     2821 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca
     2881 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
     2941 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     3001 ttcccagtca cgacgttgta aaacgacggc cagtgaattg tcgacttctg aggcggaaag
     3061 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     3121 agaagtatgc aaagcatgca tctcaattag tcagcaagga aagtccccag gctccccagc
     3181 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     3241 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3301 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3361 gtgaagaggc ttttttggag gcctaggctt ttgcaaaaag ctccggatcg atcctgagaa
     3421 cttcagggtg agtttgggga cccttgattg ttctttcttt ttcgctattg taaaattcat
     3481 gttatatgga gggggcaaag ttttcagggt gttgtttaga atgggaagat gtcccttgta
     3541 tcaccatgga ccctcatgat aattttgttt ctttcacttt ctactctgtt gacaaccatt
     3601 gtctcctctt attttctttt cattttctgt aactttttcg ttaaacttta gcttgcattt
     3661 gtaacgaatt tttaaattca cttttgttta tttgtcagat tgtaagtact ttctctaatc
     3721 actttttttt caaggcaatc agggtatatt atattgtact tcagcacagt tttagagaac
     3781 aattgttata attaaatgat aaggtagaat atttctgcat ataaattctg gctggcgtgg
     3841 aaatattctt attggtagaa acaactacat cctggtcatc atcctgcctt tctctttatg
     3901 gttacaatga tatacactgt ttgagatgag gataaaatac tctgagtcca aaccgggccc
     3961 ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca acgtgctggt
     4021 tattgtgctg tctcatcatt ttggcaaaga attgtaatac gactcactat agggcgaatt
     4081 accgccgcca tgggctaccc atacgatgtt cctgactatg cggaattcga gactcagttc
     4141 cgcagagggg gtctggggtg cagccctgcc agcatcaaga ggaagaagaa gagggaggac
     4201 tcaggagact ttggactcca ggtgagcact atgttctcag aggacgactt ccagtcaaca
     4261 gaaagagccc catatggtcc acaactacag tggtcccagg atctgccaag agtccaggtt
     4321 tttagagaac aggccaacct ggaggacagg agtcccagga gaacccagag gatcactgga
     4381 ggagaacaag tgctgtgggg ccccatcacc cagatatttc ccacagttcg gcctgctgac
     4441 ctaaccagag tcatcatgcc tcttgagcaa agaagtcagc actgcaagcc tgaggaaggc
     4501 cttcaggccc aagaagaaga cctgggcctg gtgggtgcac aggctctcca agctgaggag
     4561 caggaggctg ccttcttctc ctctactctg aatgtgggca ctctagagga gttgcctgct
     4621 gctgagtcac caagtcctcc ccagagtcct caggaagagt ccttctctcc cactgccatg
     4681 gatgccatct ttgggagcct atctgatgag ggctctggca gccaagaaaa ggaggggcca
     4741 agtacctcgc ctgacctgat agaccctgag tccttttccc aagatatact acatgacaag
     4801 ataattgatt tggttcattt attgctccgc aagtatcgag tcaaggggct gatcacaaag
     4861 gcagaaatgc tggggagtgt catcaaaaat tatgaggact actttcctga gatatttagg
     4921 gaagcctctg tatgcatgca actgctcttt ggcattgatg tgaaggaagt ggaccccact
     4981 agccactcct atgtccttgt cacctccctc aacctctctt atgatggcat acagtgtaat
     5041 gagcagagca tgcccaagtc tggcctcctg ataatagtcc tgggtgtaat cttcatggag
     5101 gggaactgca tccctgaaga ggttatgtgg gaagtcctga gcattatggg ggtgtatgct
     5161 ggaagggagc acttcctctt tggggagccc aagaggctcc ttacccaaaa ttgggtgcag
     5221 gaaaagtacc tggtgtaccg gcaggtgccc ggcactgatc ctgcatgcta tgagttcctg
     5281 tggggtccaa gggcccacgc tgagaccagc aagatgaaag ttcttgagta catagccaat
     5341 gccaatggga gggatcccac ttcttaccca tccctgtatg aagatgcttt gagagaggag
     5401 ggagagggag tctgagcatg agatgcaacc agggccagcg ggcagggaaa tgggccaatg
     5461 catgcttcag ggccacaccc agcagtttcc ctgtcctgtg tgaaatcagg cccattcttc
     5521 cctctgtgtt tgatgagaga agtcagtgtt ctcagtagta gaaggcacag tgaatggaag
     5581 ggaacacatt gtatactgcc tttaggtttc tcttccatcg ggtgacttgg agatttcttt
     5641 ttgtttccct ttggtaattt tcaaatattg ttcctgtaat aaaagtttta gttagcttca
     5701 acatctaagt gtatggatga tactgaccac acatgttgtt ttgcttatcc atttcaagtg
     5761 caagtgtttg ccattttgta aaacattttg ggaaatcttc catcttgctg tgatttgcaa
     5821 taggtatttt cttggagaat gtaagaactt aacaataaag ctgaactggt gttgtgaaac
     5881 agagaaataa aaggagaagg t
//
