LOCUS       sequence-176073-        5914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of full-length human MAGE-A11 amino acid 
            residues 2-429 with TA360 phosphorylation site mutated.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5914)
  AUTHORS   Bai S, Wilson EM
  TITLE     Epidermal-growth-factor-dependent phosphorylation and 
            ubiquitinylation of MAGE-11 regulates its interaction with the 
            androgen receptor.
  JOURNAL   Mol Cell Biol. 2008 Mar;28(6):1947-63. doi: 10.1128/MCB.01672-07. 
            Epub 2008 Jan 22.
  PUBMED    18212060
REFERENCE   2  (bases 1 to 5914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01672-07"; journalName: "Mol Cell Biol"; date: 
            "2008-03"; volume: "28"; issue: "6"; pages: "1947-63"
COMMENT     Sequence Label: pSG5-MAGE-TA360
FEATURES             Location/Qualifiers
     source          1..5914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    22..143
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(59..78)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     113..132
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(177..194)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(348..936)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(428..447)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1107..1898,1899..1967))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1730..1749
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1968..2072)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2140..2158
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2196..2218)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2318..2337
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2404..2859)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2541..2562)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2753..2772
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2985..3007
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2999..3016
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3000..3016
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3053..3073)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3161..3371
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        3183..3379
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3230..3365
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          3410..3982
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     3990..4009
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     4037..4056
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4037..4055
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 cgagagatct tattaaagca gaacttgttt attgcagctt ataatggtta caaataaagc
       61 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
      121 tccaaactca tcaatgtatc ttatcatgtc tggtcgactc tagactcttc cgcttcctcg
      181 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      241 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
      361 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      421 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
      481 accctgccgc ttatcggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
      541 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
      601 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
      661 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
      721 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
      781 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
      841 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
      901 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
      961 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     1021 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     1081 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     1141 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     1201 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     1261 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     1321 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1381 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1441 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1501 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     1561 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1621 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1681 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1741 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1801 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1861 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1921 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1981 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     2041 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     2101 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     2161 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     2221 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     2281 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     2341 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     2401 aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     2461 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     2521 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     2581 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     2641 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     2701 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     2761 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     2821 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg
     2881 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     2941 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     3001 taaaacgacg gccagtgaat tgtcgacttc tgaggcggaa agaaccagct gtggaatgtg
     3061 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     3121 catctcaatt agtcagcaag gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     3181 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     3241 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     3301 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaagag gcttttttgg
     3361 aggcctaggc ttttgcaaaa agctccggat cgatcctgag aacttcaggg tgagtttggg
     3421 gacccttgat tgttctttct ttttcgctat tgtaaaattc atgttatatg gagggggcaa
     3481 agttttcagg gtgttgttta gaatgggaag atgtcccttg tatcaccatg gaccctcatg
     3541 ataattttgt ttctttcact ttctactctg ttgacaacca ttgtctcctc ttattttctt
     3601 ttcattttct gtaacttttt cgttaaactt tagcttgcat ttgtaacgaa tttttaaatt
     3661 cacttttgtt tatttgtcag attgtaagta ctttctctaa tcactttttt ttcaaggcaa
     3721 tcagggtata ttatattgta cttcagcaca gttttagaga acaattgtta taattaaatg
     3781 ataaggtaga atatttctgc atataaattc tggctggcgt ggaaatattc ttattggtag
     3841 aaacaactac atcctggtca tcatcctgcc tttctcttta tggttacaat gatatacact
     3901 gtttgagatg aggataaaat actctgagtc caaaccgggc ccctctgcta accatgttca
     3961 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
     4021 ttttggcaaa gaattgtaat acgactcact atagggcgaa ttcgcggccg cgtcgacgag
     4081 tggcagaggg cagcgggtcc aggctccatg aggaggcaag ccttgggaat ctgagggatg
     4141 gagactcagt tccgcagagg gggtctgggg tgcagccctg ccagcatcaa gaggaagaag
     4201 aagagggagg actcaggaga ctttggactc caggtgagca ctatgttctc agaggacgac
     4261 ttccagtcaa cagaaagagc cccatatggt ccacaactac agtggtccca ggatctgcca
     4321 agagtccagg tttttagaga acaggccaac ctggaggaca ggagtcccag gagaacccag
     4381 aggatcactg gaggagaaca agtgctgtgg ggccccatca cccagatatt tcccacagtt
     4441 cggcctgctg acctaaccag agtcatcatg cctcttgagc aaagaagtca gcactgcaag
     4501 cctgaggaag gccttcaggc ccaagaagaa gacctgggcc tggtgggtgc acaggctctc
     4561 caagctgagg agcaggaggc tgccttcttc tcctctactc tgaatgtggg cactctagag
     4621 gagttgcctg ctgctgagtc accaagtcct ccccagagtc ctcaggaaga gtccttctct
     4681 cccactgcca tggatgccat ctttgggagc ctatctgatg agggctctgg cagccaagaa
     4741 aaggaggggc caagtacctc gcctgacctg atagaccctg agtccttttc ccaagatata
     4801 ctacatgaca agataattga tttggttcat ttattgctcc gcaagtatcg agtcaagggg
     4861 ctgatcacaa aggcagaaat gctggggagt gtcatcaaaa attatgagga ctactttcct
     4921 gagatattta gggaagcctc tgtatgcatg caactgctct ttggcattga tgtgaaggaa
     4981 gtggacccca ctagccactc ctatgtcctt gtcacctccc tcaacctctc ttatgatggc
     5041 atacagtgta atgagcagag catgcccaag tctggcctcc tgataatagt cctgggtgta
     5101 atcttcatgg aggggaactg catccctgaa gaggttatgt gggaagtcct gagcattatg
     5161 ggggtgtatg ctggaaggga gcacttcctc tttggggagc ccaagaggct ccttgcccaa
     5221 aattgggtgc aggaaaagta cctggtgtac cggcaggtgc ccggcactga tcctgcatgc
     5281 tatgagttcc tgtggggtcc aagggcccac gctgagacca gcaagatgaa agttcttgag
     5341 tacatagcca atgccaatgg gagggatccc acttcttacc catccctgta tgaagatgct
     5401 ttgagagagg agggagaggg agtctgagca tgagatgcaa ccagggccag cgggcaggga
     5461 aatgggccaa tgcatgcttc agggccacac ccagcagttt ccctgtcctg tgtgaaatca
     5521 ggcccattct tccctctgtg tttgatgaga gaagtcagtg ttctcagtag tagaaggcac
     5581 agtgaatgga agggaacaca ttgtatactg cctttaggtt tctcttccat cgggtgactt
     5641 ggagatttct ttttgtttcc ctttggtaat tttcaaatat tgttcctgta ataaaagttt
     5701 tagttagctt caacatctaa gtgtatggat gatactgacc acacatgttg ttttgcttat
     5761 ccatttcaag tgcaagtgtt tgccattttg taaaacattt tgggaaatct tccatcttgc
     5821 tgtgatttgc aataggtatt ttcttggaga atgtaagaac ttaacaataa agctgaactg
     5881 gtgttgtgaa acagagaaat aaaaggagaa ggtc
//
