LOCUS       sequence-176078-        4759 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of FKBP5 also referred to as FKBP51 with 
            N-terminal GAL4 DNA binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Su S, Blackwelder AJ, Grossman G, Minges JT, Yuan L, Young SL, 
            Wilson EM
  TITLE     Primate-specific melanoma antigen-A11 regulates isoform-specific 
            human progesterone receptor-B transactivation.
  JOURNAL   J Biol Chem. 2012 Oct 5;287(41):34809-24. doi: 
            10.1074/jbc.M112.372797. Epub 2012 Aug 13.
  PUBMED    22891251
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.372797"; journalName: "J Biol Chem"; date: 
            "2012-10-5- 5"; volume: "287"; issue: "41"; pages: "34809-24"
COMMENT     Sequence Label: GAL-FKBP51
FEATURES             Location/Qualifiers
     source          1..4759
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(55..75)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             397..837
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    2383..2517
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2420..2439)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2474..2493
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2580..2599)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2864..2881)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3035..3623)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3115..3134)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3794..4585,4586..4654))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4417..4436
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4655..4759)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacct tctgaggcgg aaagaaccag ctgtggaatg
       61 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      121 tgcatctcaa ttagtcagca aggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      181 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      241 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
      301 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgaag aggctttttt
      361 ggaggagatc taagcttgaa gcaagcctcc tgaaagatga agctactgtc ttctatcgaa
      421 caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc
      481 gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg
      541 ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga acagctattt
      601 ctactgattt ttcctcgaga agaccttgac atgattttga aaatggattc tttacaggat
      661 ataaaagcat tgttaacagg attatttgta caagataatg tgaataaaga tgccgtcaca
      721 gatagattgg cttcagtgga gactgatatg cctctaacat tgagacagca tagaataagt
      781 gcgacatcat catcggaaga gagtagtaac aaaggtcaaa gacagttgac tgtatcgccg
      841 gaattcacaa tgactactga tgaaggtgcc aagaacaatg aagaaagccc cacagccact
      901 gttgctgagc agggagagga tattacctcc aaaaaagaca ggggagtatt aaagattgtc
      961 aaaagagtgg ggaatggtga ggaaacgccg atgattggag acaaagttta tgtccattac
     1021 aaaggaaaat tgtcaaatgg aaagaagttt gattccagtc atgatagaaa tgaaccattt
     1081 gtctttagtc ttggcaaagg ccaagtcatc aaggcatggg acattggggt ggctaccatg
     1141 aagaaaggag agatatgcca tttactgtgc aaaccagaat atgcatatgg ctcggctggc
     1201 agtctcccta aaattccctc gaatgcaact ctcttttttg agattgagct ccttgatttc
     1261 aaaggagagg atttatttga agatggaggc attatccgga gaaccaaacg gaaaggagag
     1321 ggatattcaa atccaaacga aggagcaaca gtagaaatcc acctggaagg ccgctgtggt
     1381 ggaaggatgt ttgactgcag agatgtggca ttcactgtgg gcgaaggaga agaccacgac
     1441 attccaattg gaattgacaa agctctggag aaaatgcagc gggaagaaca atgtatttta
     1501 tatcttggac caagatatgg ttttggagag gcagggaagc ctaaatttgg cattgaacct
     1561 aatgctgagc ttatatatga agttacactt aagagcttcg aaaaggccaa agaatcctgg
     1621 gagatggata ccaaagaaaa attggagcag gctgccattg tcaaagagaa gggaaccgta
     1681 tacttcaagg gaggcaaata catgcaggcg gtgattcagt atgggaagat agtgtcctgg
     1741 ttagagatgg aatatggttt atcagaaaag gaatcgaaag cttctgaatc atttctcctt
     1801 gctgcctttc tgaacctggc catgtgctac ctgaagctta gagaatacac caaagctgtt
     1861 gaatgctgtg acaaggccct tggactggac agtgccaatg agaaaggctt gtataggagg
     1921 ggtgaagccc agctgctcat gaacgagttt gagtcagcca agggtgactt tgagaaagtg
     1981 ctggaagtaa acccccagaa taaggctgca agactgcaga tctccatgtg ccagaaaaag
     2041 gccaaggagc acaacgagcg ggaccgcagg atatacgcca acatgttcaa gaagtttgca
     2101 gagcaggatg ccaaggaaga ggccaataaa gcaatgggca agaagacttc agaaggggtc
     2161 actaatgaaa aaggaacaga cagtcaagca atggaagaag agaaacctga gggccacgta
     2221 taggtcgaca tatgctcgag cccatcgata atgcatccga gctctagata agtaatgatc
     2281 ataatcagcc atatcacatc tgtagaggtt ttacttgctt taaaaaacct cccacacctc
     2341 cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct
     2401 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
     2461 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcgat
     2521 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     2581 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     2641 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     2701 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     2761 cttcctaatg caggagtcgc ataagggaga gcgtcgatat gcggtgtgaa ataccgcaca
     2821 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     2881 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2941 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     3001 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3061 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3121 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3181 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     3241 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3301 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3361 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3421 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     3481 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3541 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3601 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3661 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3721 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3781 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3841 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3901 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3961 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     4021 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4081 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     4141 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4201 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4261 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4321 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4381 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     4441 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4501 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4561 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4621 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4681 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4741 aacaaatagg ggttccgcg
//
