LOCUS       sequence-176081-        7352 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of full-length 410 amino acid SUV39H2 histone 
            methyltransferase from pSG5, includes 5' and 3' noncoding regions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Askew EB, Bai S, Parris AB, Minges JT, Wilson EM
  TITLE     Androgen receptor regulation by histone methyltransferase Suppressor
            of variegation 3-9 homolog 2 and Melanoma antigen-A11.
  JOURNAL   Mol Cell Endocrinol. 2017 Mar 5;443:42-51. doi: 
            10.1016/j.mce.2016.12.028. Epub 2016 Dec 29.
  PUBMED    28042025
REFERENCE   2  (bases 1 to 7352)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Endocrinol."; date: "2017-03-5"; volume: "443:42-51. doi"; pages: " 
            10.1016/j.mce.2016.12.028. Epub 2016 Dec 29"
COMMENT     Sequence Label: pSG5-SUV39H2-FL
FEATURES             Location/Qualifiers
     source          1..7352
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        23..219
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          250..822
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     830..849
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     877..896
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        877..895
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    916..940
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     942..958
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4155..4171)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4155..4171)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(4172..4196)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    4214..4335
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4251..4270)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4305..4324
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4369..4386)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4540..5128)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4620..4639)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5299..6090,6091..6159))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5922..5941
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6160..6264)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6332..6350
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6388..6410)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6510..6529
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6596..7051)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6733..6754)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6945..6964
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7177..7199
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7192..7208
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7245..7265)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      181 tagtgaagag gcttttttgg aggcctaggc ttttgcaaaa agctccggat cgatcctgag
      241 aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat tgtaaaattc
      301 atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag atgtcccttg
      361 tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg ttgacaacca
      421 ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt tagcttgcat
      481 ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta ctttctctaa
      541 tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca gttttagaga
      601 acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc tggctggcgt
      661 ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc tttctcttta
      721 tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc caaaccgggc
      781 ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg
      841 gttattgtgc tgtctcatca ttttggcaaa gaattgtaat acgactcact atagggcgaa
      901 ttcggatcca gatccacaag tttgtacaaa aaagcaggct tgtaaaacga cggccagtaa
      961 ctataacggt cctaaggtag cgaggcctgg gtggcgaatt cggcacgagg cggggccgag
     1021 gcgcgaggag gtgaggctgg agcgcggccc cctcgccttc cctgttccca ggcaagctcc
     1081 caaggcccgg gcggcggggc cgtcccgcgg gccagccaga tggcgacgtg gcggttcccc
     1141 gcccgccgcg accccaactc cgggacgcac gctgcggacg cctatcctcc cccaggccgc
     1201 tgacccgcct ccctgcccgg ccggctcccg ccgcggagga tatggaatat tatcttgtaa
     1261 aatggaaagg atggccagat tctacaaata cttgggaacc tttgcaaaat ctgaagtgcc
     1321 cgttactgct tcagcaattc tctaatgaca agcataatta tttatctcag gtaaagaaag
     1381 gcaaagcaat aactccaaaa gacaataaca aaactttgaa acctgccatt gctgagtaca
     1441 ttgtgaagaa ggctaaacaa aggatagctc tgcagagatg gcaagatgaa ctcaacagaa
     1501 gaaagaatca taaaggaatg atatttgttg aaaatactgt tgatttagag ggcccacctt
     1561 cagacttcta ttacattaac gaatacaaac cagctcctgg aatcagctta gtcaatgaag
     1621 ctacctttgg ttgttcatgc acagattgct tctttcaaaa atgttgtcct gctgaagctg
     1681 gagttctttt ggcttataat aaaaaccaac aaattaaaat cccacctggt actcccatct
     1741 atgaatgcaa ctcaaggtgt cagtgtggtc ctgattgtcc caataggatt gtacaaaaag
     1801 gcacacagta ttcgctttgc atctttcgaa ctagcaatgg acgtggctgg ggtgtaaaga
     1861 cccttgtgaa gattaaaaga atgagttttg tcatggaata tgttggagag gtaatcacaa
     1921 gtgaagaagc tgaaagacga ggacagttct atgacaacaa gggaatcacg tatctctttg
     1981 atctggacta tgagtctgat gaattcacag tggatgcggc tcgatacggc aatgtgtctc
     2041 attttgtgaa tcacagctgt gacccaaatc ttcaggtgtt caatgttttc attgataacc
     2101 tcgatactcg tcttccccga atagcattgt tttccacaag aaccataaat gctggagaag
     2161 agctgacttt tgattatcaa atgaaaggtt ctggagatat atcttcagat tctattgacc
     2221 acagcccagc caaaaagagg gtcagaacag tatgtaaatg tggagctgtg acttgcagag
     2281 gttacctcaa ctgaactttt tcaggaaata gagctgatga ttataatatt tttttcctaa
     2341 tgttaacatt tttaaaaata catatttggg actcttatta tcaaggttct acctatgtta
     2401 atttacaatt catgtttcaa gacatttgcc aaatgtatta ccgatgcctc tgaaaagggg
     2461 gtcactgggt ctcatagact gatatgaagt cgacatattt atagtgctta gagaccaaac
     2521 taatggaagg cagactattt acagcttagt atatgtgtac ttaagtctat gtgaacagag
     2581 aaatgcctcc cgtagtgttt gaaagcgtta agctgataat gtaattaaca actgctgaga
     2641 gatcaaagat tcaacttgcc atacacctca aattcggaga aacagttaat ttgggcaaat
     2701 ctacagttct gtttttgcta ctctattgtc attcctgttt aatactcact gtacttgtat
     2761 ttgagacaaa taggtgatac tgaattttat actgttttct acttttccat taaaacattg
     2821 gcacctcaat gataaagaaa tttaaggtat aaaattaaat gtaaaaatta atttcagctt
     2881 catttcgtat ttcgaagcaa tctagactgt tgtgatgagt gtatgtctga acctgtaatt
     2941 cttaaaagac ttcttaatct tctagaagaa aaatctccga agagctctct ctagaagtcc
     3001 aaaatggcta gccattatgc ttctttgaaa ggacatgata atgggaccag gatggttttt
     3061 tggagtacca agcaagggga atggagcact ttaagggcgc ctgttagtaa catgaattgg
     3121 aaatctgtgt cgagtacctc tgatctaaac ggtaaaacaa gctgcctgga gagcagctgt
     3181 acctaacaat actgtaatgt acattaacat tacagcctct caatttcagg caggtgtaac
     3241 agttcctttc caccagattt aatattttta tacttcctgc aggttcttct taaaaagtaa
     3301 tctatatttt tgaactgata cttgttttat acataaattt tttttagatg tgataaagct
     3361 aaacttggcc aaagtgtgtg cctgaattat tagacctttt tattagtcaa cctacgaaga
     3421 ctaaaataga atatattagt tttcaaggga gtgggaggct tccaacatag tattgaatct
     3481 caggaaaaac tattctttca tgtctgattc tgagatttct aattgtgttg tgaaaatgat
     3541 aaatgcagca aatctagctt tcagtattcc taatttttac ctaagctcat tgctccaggc
     3601 tttgattacc taaaataagc ttggataaaa ttgaaccaac ttcaagaatg cagcacttct
     3661 taatctttag ctctttcttg ggagaagcta gactttattc attatattgc tatgacaact
     3721 tcactctttc ataatatata ggataaattg tttacatgat tggaccctca gattctgtta
     3781 accaaaattg cagaatgggg ggccaggcct gtgtggtggc tcacacctgt gatcccagca
     3841 ctttgggagg ctgaggtagg aggatcacgt gaggtcggga gttcaagacc agcctggcca
     3901 tcatggtgaa accctgtctc tactgaaaat acaaaaatta gccgggcgtg gtggcacacg
     3961 cctgtagtcc cagctactca ggaggctgag gcaggagaat cacttgaatt caggaggcgg
     4021 aggttgcagt gagccaagat cataccactg cactgcagcc tgagtgacac agtaagactg
     4081 tctccaaaaa aaaaaaaaaa aaactcgagg catctatgtc gggtgcggag aaagaggtaa
     4141 tgaaatggca catggtcata gctgtttcct gacccagctt tcttgtacaa agtggtagat
     4201 cttattaaag cagaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     4261 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     4321 catcaatgta tcttatcatg tctggtcgac tctagactct tccgcttcct cgctcactga
     4381 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     4441 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     4501 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     4561 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     4621 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     4681 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     4741 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     4801 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     4861 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     4921 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     4981 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     5041 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     5101 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     5161 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     5221 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     5281 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     5341 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     5401 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     5461 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     5521 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     5581 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     5641 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     5701 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     5761 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     5821 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     5881 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     5941 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     6001 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     6061 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     6121 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     6181 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     6241 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     6301 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
     6361 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     6421 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     6481 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
     6541 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
     6601 gtaatcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt
     6661 aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg
     6721 ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc
     6781 aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc accctaatca
     6841 agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga
     6901 tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa
     6961 ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc
     7021 gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt
     7081 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     7141 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     7201 cggccagtga attgtcgact tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt
     7261 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7321 ttagtcagca aggaaagtcc ccaggctccc ca
//
