LOCUS       sequence-176084-        4616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of full-length 410 amino acid SUV39H2 histone 
            methyltransferase linked to GAL4 DNA binding domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4616)
  AUTHORS   Askew EB, Bai S, Parris AB, Minges JT, Wilson EM
  TITLE     Androgen receptor regulation by histone methyltransferase Suppressor
            of variegation 3-9 homolog 2 and Melanoma antigen-A11.
  JOURNAL   Mol Cell Endocrinol. 2017 Mar 5;443:42-51. doi: 
            10.1016/j.mce.2016.12.028. Epub 2016 Dec 29.
  PUBMED    28042025
REFERENCE   2  (bases 1 to 4616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Endocrinol."; date: "2017-03-5"; volume: "443:42-51. doi"; pages: " 
            10.1016/j.mce.2016.12.028. Epub 2016 Dec 29"
COMMENT     Sequence Label: GAL-SUV39H2-L
FEATURES             Location/Qualifiers
     source          1..4616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(55..75)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             397..837
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     polyA_signal    2240..2374
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2277..2296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2331..2350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2437..2456)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2721..2738)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2892..3480)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2972..2991)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3651..4442,4443..4511))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4274..4293
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4512..4616)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacct tctgaggcgg aaagaaccag ctgtggaatg
       61 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      121 tgcatctcaa ttagtcagca aggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      181 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      241 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
      301 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgaag aggctttttt
      361 ggaggagatc taagcttgaa gcaagcctcc tgaaagatga agctactgtc ttctatcgaa
      421 caagcatgcg atatttgccg acttaaaaag ctcaagtgct ccaaagaaaa accgaagtgc
      481 gccaagtgtc tgaagaacaa ctgggagtgt cgctactctc ccaaaaccaa aaggtctccg
      541 ctgactaggg cacatctgac agaagtggaa tcaaggctag aaagactgga acagctattt
      601 ctactgattt ttcctcgaga agaccttgac atgattttga aaatggattc tttacaggat
      661 ataaaagcat tgttaacagg attatttgta caagataatg tgaataaaga tgccgtcaca
      721 gatagattgg cttcagtgga gactgatatg cctctaacat tgagacagca tagaataagt
      781 gcgacatcat catcggaaga gagtagtaac aaaggtcaaa gacagttgac tgtatcgccg
      841 gaattcccgg ggatcctaat ggcggcggtc ggggccgagg cgcgaggagc ttggtgtgtg
      901 ccttgcctag tttcacttga tactcttcag gaattatgta gaaaagaaaa gctcacatgt
      961 aaatcgattg gaatcaccaa aaggaatcta aacaattatg aggtggaata cttgtgtgac
     1021 tacaaggtag taaaggatat ggaatattat cttgtaaaat ggaaaggatg gccagattct
     1081 acaaatactt gggaaccttt gcaaaatctg aagtgcccgt tactgcttca gcaattctct
     1141 aatgacaagc ataattattt atctcaggta aagaaaggca aagcaataac tccaaaagac
     1201 aataacaaaa ctttgaaacc tgccattgct gagtacattg tgaagaaggc taaacaacgg
     1261 atagctctgc agagatggca agatgaactc aacagaagaa agaatcataa aggaatgata
     1321 tttgttgaaa atactgttga tttagagggc ccaccttcag acttctatta cattaacgaa
     1381 tacaaaccag ctcctggaat cagcttagtc aatgaagcta cctttggttg ttcatgcaca
     1441 gattgcttct ttcaaaaatg ttgtcctgct gaagctggag ttcttttggc ttataataaa
     1501 aaccaacaaa ttaaaatccc acctggtact cccatctatg aatgcaactc aaggtgtcag
     1561 tgtggtcctg attgtcccaa taggattgta caaaaaggca cacagtattc gctttgcatc
     1621 tttcgaacta gcaatggacg tggctggggt gtaaagaccc ttgtgaagat taaaagaatg
     1681 agttttgtca tggaatatgt tggagaggta atcacaagtg aagaagctga aagacgagga
     1741 cagttctatg acaacaaggg aatcacgtat ctctttgatc tggactatga gtctgatgaa
     1801 ttcacagtgg atgcggctcg atacggcaat gtgtctcatt ttgtgaatca cagctgtgac
     1861 ccaaatcttc aggtgttcaa tgttttcatt gataacctcg atactcgtct tccccgaata
     1921 gcattgtttt ccacaagaac cataaatgct ggagaagagc tgacttttga ttatcaaatg
     1981 aaaggttctg gagatatatc ttcagattct attgaccaca gcccagccaa aaagagggtc
     2041 agaacagtat gtaaatgtgg agctgtgact tgcagaggtt acctcaactg actcgagccc
     2101 atcgataatg catccgagct ctagataagt aatgatcata atcagccata tcacatctgt
     2161 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     2221 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     2281 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     2341 caaactcatc aatgtatctt atcatgtctg gatcgatcct ctacgccgga cgcatcgtgg
     2401 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     2461 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     2521 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     2581 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     2641 agggagagcg tcgatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
     2701 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2761 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2821 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2881 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2941 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     3001 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     3061 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     3121 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     3181 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     3241 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     3301 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     3361 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     3421 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     3481 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     3541 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     3601 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3661 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3721 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3781 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3841 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3901 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3961 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     4021 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     4081 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     4141 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     4201 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     4261 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     4321 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     4381 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     4441 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     4501 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     4561 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcg
//
