LOCUS       sequence-176085-        5792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5792)
  TITLE     Unpublished Plasmids
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FLAG-Foxo4
FEATURES             Location/Qualifiers
     source          1..5792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             97..689
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    752..951
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     primer_bind     complement(1121..1141)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1126..1455
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1306..1441
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          1494..1590
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      1637..1646
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1646..1669
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    3174..3308
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3198..3217)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3252..3271
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3451..3473)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3559..3699
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3614..3633
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3714..3731)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3885..4473)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3965..3984)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4644..5435,5436..5504))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5267..5286
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5505..5609)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     5677..5695
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tctagttttg actcaacaat atcaccagct gaagcctata gagtacgagc catagataaa
       61 ataaaagatt ttatttagtc tccagaaaaa ggggggaatg aaagacccca cctgtaggtt
      121 tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat aactgagaat
      181 agagaagttc agatcaaggt caggaacaga gaaacagctg aatatgggcc aaacaggata
      241 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagttggaac agctgaatat
      301 aggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat
      361 ggtccccaga tgcggtccag ccctcagcag tttctagaga accatcagat gtttccaggg
      421 tgccccaagg acctgaaatg accctgtgcc ttatttgaac taaccaatca gttcgcttct
      481 cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa agagcccaca acccctcact
      541 cggcgcgcca gtcctccgag tgactgagtc gcccgggtac ccgtgtatcc aataaaccct
      601 cttgcagttg catccgactt gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactacccg tcagcggggg tctttcattt gggggctcgt ccgggatcgg gagacccctg
      721 cccagggacc accgacccac caccgggagg taagctggcc agcaacttat ctgtgtctgt
      781 ccgattgtct agtgtctatg actgatttta tgcgcctgcg tcggtactag ttagctaact
      841 agctctgtat ctggcggacc cgtggtggaa ctgacgagtt cggaacaccc ggccgcaacc
      901 ctgggagacg tcccagggac ttcgggggcc gtttttgtgg cccgacctga gtccaaaaat
      961 cccgatcgtt ttggactctt tggtgcaccc cccttagagg agggatatgt ggttctggta
     1021 ggagacgaga acctaaaaca gttcccgcct ccgtctgaat ttttgctttc ggtttggaac
     1081 cgaagccgcg ccgcgcgtct tgtctgccaa gcttggctgt ggaatgtgtg tcagttaggg
     1141 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     1201 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     1261 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     1321 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     1381 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     1441 ctaggctttt gcaaaaagct cctcgaggaa ctgaaaaacc agaaagttaa ctggtaagtt
     1501 tagtcttttt gtcttttatt tcaggtcccg gatccggtgg tggtgcaaat caaagaactg
     1561 ctcctcagtg gatgttgcct ttacttctag gcctgtacgg aagtgttact tctgctctaa
     1621 aagctgcgga attgccgcca ccatggatta caaggacgac gacgataaga tcgaattcgc
     1681 cgcgatcata gacctagatc ccgacttcga accccagagc cgtccccgct cctgcacctg
     1741 gccccttccc cgaccagaga tcgctaacca gccgtccgag ccgcccgagg tggagccaga
     1801 tctgggggaa aaggtacaca cggaggggcg ctcagagccg atcctgttgc cctctcggct
     1861 cccagagccg gccgggggcc cccagcccgg aatcctgggg gctgtaacag gtcctcggaa
     1921 gggaggctcc cgccggaatg cctggggaaa tcagtcatat gcagaactca tcagccaggc
     1981 cattgaaagc gccccggaga agcgactgac acttgcccag atctacgagt ggatggtccg
     2041 tactgtaccc tacttcaagg acaagggtga cagcaacagc tcagcaggat ggaagaactc
     2101 gatccgccac aacctgtccc tgcacagcaa gttcatcaag gttcacaacg aggccaccgg
     2161 caaaagctct tggtggatgc tgaaccctga gggaggcaag agcggcaaag ccccccgccg
     2221 ccgggccgcc tccatggata gcagcagcaa gctgctccgg ggccgcagta aagcccccaa
     2281 gaagaaacca tctgtgctgc cagctccacc cgaaggtgcc actccaacga gccctgtcgg
     2341 ccactttgcc aagtggtcag gcagcccttg ctctcgaaac cgtgaagaag ccgatatgtg
     2401 gaccaccttc cgtccacgaa gcagttcaaa tgccagcagt gtcagcaccc ggctgtcccc
     2461 cttgaggcca gagtctgagg tgctggcgga ggaaatacca gcttcagtca gcagttatgc
     2521 agggggtgtc cctcccaccc tcaatgaagg tctagagctg ttagatgggc tcaatctcac
     2581 ctcttcccat tccctgctat ctcggagtgg tctctctggc ttctctttgc agcatcctgg
     2641 ggttaccggc cccttacaca cctacagcag ctcccttttc agcccagcag aggggcccct
     2701 gtcagcagga gaagggtgct tctccagctc ccaggctctg gaggccctgc tcacctctga
     2761 tacgccacca ccccctgctg acgtcctcat gacccaggta gatcccattc tgtcccaggc
     2821 tccgactctt ctgttgctgg gggggcttcc ttcctccagt aagctggcca cgggcgtcgg
     2881 cctgtgtccc aagcccctag aggctccagg ccccagcagt ctggttccca ccctttctat
     2941 gatagcacca cctccagtca tggcaagtgc ccccatcccc aaggctctgg ggactcctgt
     3001 gctcacaccc cctactgaag ctgcaagcca agacagaatg cctcaggatc tagatcttga
     3061 tatgtatatg gagaacctgg agtgtgacat ggataacatc atcagtgacc tcatggatga
     3121 gggcgaggga ctggacttca actttgagcc agatccctga ggatctcaat tccgatccag
     3181 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     3241 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     3301 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
     3361 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatccggctg
     3421 cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     3481 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     3541 tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac
     3601 tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa
     3661 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc
     3721 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     3781 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     3841 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     3901 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     3961 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4021 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4081 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4141 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4201 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     4261 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     4321 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     4381 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     4441 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     4501 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     4561 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     4621 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     4681 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     4741 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     4801 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     4861 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     4921 tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc
     4981 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     5041 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5101 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5161 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     5221 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca
     5281 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     5341 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     5401 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     5461 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     5521 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     5581 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     5641 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     5701 cgtcttcaag aattggtcga ccaattctca tgtttgacag cttatcatcg attagtccaa
     5761 tttgttaaag acaggatatc agtggtccag gc
//
